中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2012年
1期
55-60
,共6页
察鹏飞%高建华%陈阳%鲁峰
察鵬飛%高建華%陳暘%魯峰
찰붕비%고건화%진양%로봉
组织工程%脂肪组织%脂肪来源干细胞%细胞外基质%支架
組織工程%脂肪組織%脂肪來源榦細胞%細胞外基質%支架
조직공정%지방조직%지방래원간세포%세포외기질%지가
Tissue engineering%Adipose tissue%Adipose-derived stem cells%Extracellular%Matrix%Scaffold
目的 探讨从人脂肪组织中提取细胞外基质并构建支架的方法,研究其结构特点,分析其作为脂肪组织工程支架的可行性.方法 收集抽脂术患者的脂肪组织,共7例,每例约310 ml,10 ml用来分离培养脂肪来源干细胞,300 ml提取细胞外基质,并制备成粉末状,扫描电镜观察其表面结构.DiI荧光标记脂肪来源干细胞,统计荧光标记后细胞存活率；将荧光标记前、后的细胞与支架粘附,检测其粘附率,所得数据用SPSS 13.0软件两样本t检验进行统计学比较,分析标记前后细胞粘附率有无差异；荧光显微镜下观察细胞在支架表面生长情况.结果 从人脂肪组织中提取得到脂肪来源干细胞和细胞外基质粉末.脂肪来源干细胞具有成脂、成软骨和成骨分化的能力；扫描电镜观察细胞外基质粉末具有多孔、粗糙表面和光滑表面的结构.脂肪来源干细胞与支架粘附较好；DiI标记前、后细胞粘附率分别为(88.81±4.81)％和(86.48±4.58)％,两样本t检验,P=0.371,差异无统计学意义(P＞0.05).荧光显微镜下脂肪来源干细胞在细胞外基质支架上生长状态良好.结论 人脂肪组织细胞外基质粉末容易获取,形状和颗粒体积具有高度的多样性,表面积较大,有利于脂肪干细胞的粘附和增殖,可作为一种较理想的脂肪组织工程支架材料.
目的 探討從人脂肪組織中提取細胞外基質併構建支架的方法,研究其結構特點,分析其作為脂肪組織工程支架的可行性.方法 收集抽脂術患者的脂肪組織,共7例,每例約310 ml,10 ml用來分離培養脂肪來源榦細胞,300 ml提取細胞外基質,併製備成粉末狀,掃描電鏡觀察其錶麵結構.DiI熒光標記脂肪來源榦細胞,統計熒光標記後細胞存活率；將熒光標記前、後的細胞與支架粘附,檢測其粘附率,所得數據用SPSS 13.0軟件兩樣本t檢驗進行統計學比較,分析標記前後細胞粘附率有無差異；熒光顯微鏡下觀察細胞在支架錶麵生長情況.結果 從人脂肪組織中提取得到脂肪來源榦細胞和細胞外基質粉末.脂肪來源榦細胞具有成脂、成軟骨和成骨分化的能力；掃描電鏡觀察細胞外基質粉末具有多孔、粗糙錶麵和光滑錶麵的結構.脂肪來源榦細胞與支架粘附較好；DiI標記前、後細胞粘附率分彆為(88.81±4.81)％和(86.48±4.58)％,兩樣本t檢驗,P=0.371,差異無統計學意義(P＞0.05).熒光顯微鏡下脂肪來源榦細胞在細胞外基質支架上生長狀態良好.結論 人脂肪組織細胞外基質粉末容易穫取,形狀和顆粒體積具有高度的多樣性,錶麵積較大,有利于脂肪榦細胞的粘附和增殖,可作為一種較理想的脂肪組織工程支架材料.
목적 탐토종인지방조직중제취세포외기질병구건지가적방법,연구기결구특점,분석기작위지방조직공정지가적가행성.방법 수집추지술환자적지방조직,공7례,매례약310 ml,10 ml용래분리배양지방래원간세포,300 ml제취세포외기질,병제비성분말상,소묘전경관찰기표면결구.DiI형광표기지방래원간세포,통계형광표기후세포존활솔；장형광표기전、후적세포여지가점부,검측기점부솔,소득수거용SPSS 13.0연건량양본t검험진행통계학비교,분석표기전후세포점부솔유무차이；형광현미경하관찰세포재지가표면생장정황.결과 종인지방조직중제취득도지방래원간세포화세포외기질분말.지방래원간세포구유성지、성연골화성골분화적능력；소묘전경관찰세포외기질분말구유다공、조조표면화광활표면적결구.지방래원간세포여지가점부교호；DiI표기전、후세포점부솔분별위(88.81±4.81)％화(86.48±4.58)％,량양본t검험,P=0.371,차이무통계학의의(P＞0.05).형광현미경하지방래원간세포재세포외기질지가상생장상태량호.결론 인지방조직세포외기질분말용역획취,형상화과립체적구유고도적다양성,표면적교대,유리우지방간세포적점부화증식,가작위일충교이상적지방조직공정지가재료.
Objective To investigate the feasibility of constructing scaffold for tissue engineering with human extracellular matrix from adipose tissue.Methods Fresh human adipose tissue was obtained by liposuction in 7 women who undergone liposuction.One part of the fat was used to isolate the adiposederived stem cells(ADSCs),the other part was used to extract human extracellular matrix powder.After removing blood and oil components,the tissue was homogenized,centrifuged,freeze-dried,and crushed to powder by instrument.The structure of human ECM powder was observed with electron microscopy. The ADSCs were seeded and attached to the human extracellular matrix powder before and after labeled with fluorescent DiI,respectively. The adhesion rate was detected. The adhesion and growth of ADSCs were observed with Fluorescence microscope. The adhesion rate before and after DiI labeling was analyzed statistically with two-sample test of SPSS 13.0. Results The ADSCs and human extracellular matrix powder were obtained successfully from adipose tissue. The ADSCs could be differentiated into adipose cells,bone cells and chondrocytes.SEM images showed that the power had both rugged and smooth surface with a porous structure characteristics.ADSCs could adhere to the scaffold easily,and the adhesion rate was (88.81 -± 4.81 )％ and (86.48 ± 4.58)％ before and after DiI labeling.There was no difference between two groups. DiI labeled ADSCs were adhered to extracellular matrix scaffold and could grow in good condition.Conclusions Human adipose tissue extracellular matrix powder was easy to obtain,with diversity in size and shape which provided excellent substrates for cell adhesion and growth.It could be an ideal adipose tissue engineering scaffold.
