CAJ | 학술논문

目的：研究bcl-xs基因对喜树碱(CPT)诱导鼻咽癌CNE-2Z细胞凋亡的影响。方法：利用脂质体LipofectAmine将含有人bcl-xs基因的重组真核表达质粒pcDNA3xs导入人鼻咽癌低分化上皮细胞株CNE-2Z，G418筛选培养后，经Western blot检测bcl-xs的表达。以不含有bcl-xs基因的pcDNA3质粒转染CNE-2Z作为对照细胞。喜树碱处理两种细胞一定时间后，通过激光流式细胞仪检测凋亡细胞百分比。结果：Western blot检测证实获得稳定表达bcl-xs的细胞，经过0.5μmol/L,1.0μmol/L CPT处理24小时后，激光流式细胞仪检测的凋亡峰值均高于对照细胞。结论：bcl-xs能显著增加鼻咽癌CNE-2Z细胞对喜树碱诱导凋亡的敏感性。
목적：연구bcl-xs기인대희수감(CPT)유도비인암CNE-2Z세포조망적영향。방법：이용지질체LipofectAmine장함유인bcl-xs기인적중조진핵표체질립pcDNA3xs도입인비인암저분화상피세포주CNE-2Z，G418사선배양후，경Western blot검측bcl-xs적표체。이불함유bcl-xs기인적pcDNA3질립전염CNE-2Z작위대조세포。희수감처리량충세포일정시간후，통과격광류식세포의검측조망세포백분비。결과：Western blot검측증실획득은정표체bcl-xs적세포，경과0.5μmol/L,1.0μmol/L CPT처리24소시후，격광류식세포의검측적조망봉치균고우대조세포。결론：bcl-xs능현저증가비인암CNE-2Z세포대희수감유도조망적민감성。
Objective: In order to detect the effect of bcl-xs on camptothecin-induced apoptosis in human nasopharyngeal carcinoma CNE-2Z cells in vitro.Methods:bcl-xs gene-bearing mammalian expression vector(pcDNA3xs)was transfected into CNE-2Z cells using LipofectAmine.The expression of bcl-xs was determined with western blot.Cells which were transfected with native pcDNA3 vector were used as control.Apoptotic cells were detected with flow cytometry after exposure to camptothecin for 24h.Results:Cell clone(CNE-2Zxs)with stable expression of bcl-xs was obtained as confirmed with western blot.Results from flow cytometry analysis showed a significant increase of apoptotic cells in CNE-2Zxs as compared with CNE-2Zneo after treatment with the same dose of camptothecin.Conclusion:Exogenous bcl-xs expression sensitized nasopharyngeal carcinoma CNE-2Z cells to camptothecin-induced apoptosis.