CAJ | 학술논문

目的　探讨PVN不同亚核在调控脾脏免疫功能中的作用。方法　4只SD大鼠脾脏内注射假狂犬病毒（PRV）96 h后，采用免疫组化法和体视学方法研究被假狂犬病毒跨突触感染的神经元在PVN不同亚核内的分布特点及其与PVN内AVP神经元位置配布间的相互关系。结果　PRV感染的神经元主要聚集于PVN尾段的尾侧大细胞亚核（PM）和外侧小细胞亚核（LP）（占总面积的73.49％），少量分布于喙段的前小细胞亚核（LP）、中段的背侧小细胞亚核（DP）和室周小细胞亚核内（PP）。其中LP及PM内的部分PRV感染神经元分布在AVP阳性神经元聚集的区域内。结论　PVN对脾脏免疫功能的调控除了传统神经内分泌途径外，还可能存在下列途径：PM内的AVP阳性神经元和LP、DP及AP内的神经元通过向延髓背侧及脊髓中间外侧柱的投射，然后再通过副交感和交感神经的直接神经支配途径调控脾脏的功能。
목적　탐토PVN불동아핵재조공비장면역공능중적작용。방법　4지SD대서비장내주사가광견병독（PRV）96 h후，채용면역조화법화체시학방법연구피가광견병독과돌촉감염적신경원재PVN불동아핵내적분포특점급기여PVN내AVP신경원위치배포간적상호관계。결과　PRV감염적신경원주요취집우PVN미단적미측대세포아핵（PM）화외측소세포아핵（LP）（점총면적적73.49％），소량분포우훼단적전소세포아핵（LP）、중단적배측소세포아핵（DP）화실주소세포아핵내（PP）。기중LP급PM내적부분PRV감염신경원분포재AVP양성신경원취집적구역내。결론　PVN대비장면역공능적조공제료전통신경내분비도경외，환가능존재하렬도경：PM내적AVP양성신경원화LP、DP급AP내적신경원통과향연수배측급척수중간외측주적투사，연후재통과부교감화교감신경적직접신경지배도경조공비장적공능。
Objective　To investigate the possible roles of the different subnuclei of hypothalamic paraventricular nucleus （PNV） which may regulate splenic immune function. Methods　4 SD rats received splenic injection of pseudorabies virus（PRV）.96 hours after injection， a block containing hypothalamus was taken and cut into 40 μm sections with cryostat. Immunohistochemical technique and stereology method were used to detect the distributing character of transsynaptic PRV infection neurons in the PVN and the localization relationship between PRV neurons and AVP neurons in the PVN.Results　Transsynaptic PRV infection neurons distributed through out the PVN， but mainly concentrated in the lateral parvocellular （LP） subnucleus of PVN and posterior magnocellular （PM） subnuclei of PVN （occupied 73.49％ of total surface area），a few distributed in the anterior， dorsal and periventricular parvocellular （AP，DP and PP）nuclei of PVN，few scatted in the rest of nuclei of PVN. In the LP and PM some of the PRV neurons distributed among the AVP neurons.Conclusions　Besides the classical neuro-immuno-endocrine modulation， the PVN may regulate the splenic immune function through direct innervation .The AVP neurons in the PM and neurons projecting to the proganglionic neurons in the dorsal medulla and lateral horn of spinal cord in the LP，DP and AP regulate the immune function of spleen through sympathetic and para-sympathetic innovation.