CAJ | 학술논문

背景:虽然人们对铅毒性引起机体的损害有了较深入认识,但铅负荷对T细胞淋巴细胞亚群的影响还不十分明确.目的:评估血铅对儿童T细胞亚群的影响,以及血铅临界值与T细胞亚群的关系.设计:对比观察.单位:山西省儿童医院妇幼保健院分子微生物研究所.对象:选择2003-05/2003-10太原市南郊和北郊区儿童60人,男32人,女28人;年龄3～6岁,平均(4.5±1.5)岁.监护人均对检测项目知情同意.根据血铅值将儿童分为3组:≥0.48μmol/L组(n=13),≥0.24 μmol/L,＜0.48μmol/L组(n=20),＜0.24μmol/L组(n=27).方法:①采用石墨炉原子吸收光谱法测定血铅水平.T细胞亚群检测用免疫荧光法.②计量资料差异性比较采用t检验.血铅与T细胞亚群关系采用直线相关分析.主要观察指标:①不同血铅水平儿童T细胞亚群表达比较.②血铅水平与T细胞亚群的关系.结果:儿童60人均进入结果分析.①≥0.48 μmol/L组儿童CD3表达及CD4/CD8比值明显低于＜0.24 μmol/L组(t=3.27,3.07,P＜0.01).②血铅值≥0.48 μmol/L时血铅水平与CD3表达及CD4与CD8比值呈显著负相关(r=-0.689,-0.674,P＜0.01),与CD8表达呈显著正相关(r=0.748,P＜0.01).结论:血铅值水平≥0.48μmol/L,可使外周血CD3表达及CD4与CD8比值下降,对小儿免疫功能产生不良影响.
배경:수연인문대연독성인기궤체적손해유료교심입인식,단연부하대T세포림파세포아군적영향환불십분명학.목적:평고혈연대인동T세포아군적영향,이급혈연림계치여T세포아군적관계.설계:대비관찰.단위:산서성인동의원부유보건원분자미생물연구소.대상:선택2003-05/2003-10태원시남교화북교구인동60인,남32인,녀28인;년령3～6세,평균(4.5±1.5)세.감호인균대검측항목지정동의.근거혈연치장인동분위3조:≥0.48μmol/L조(n=13),≥0.24 μmol/L,＜0.48μmol/L조(n=20),＜0.24μmol/L조(n=27).방법:①채용석묵로원자흡수광보법측정혈연수평.T세포아군검측용면역형광법.②계량자료차이성비교채용t검험.혈연여T세포아군관계채용직선상관분석.주요관찰지표:①불동혈연수평인동T세포아군표체비교.②혈연수평여T세포아군적관계.결과:인동60인균진입결과분석.①≥0.48 μmol/L조인동CD3표체급CD4/CD8비치명현저우＜0.24 μmol/L조(t=3.27,3.07,P＜0.01).②혈연치≥0.48 μmol/L시혈연수평여CD3표체급CD4여CD8비치정현저부상관(r=-0.689,-0.674,P＜0.01),여CD8표체정현저정상관(r=0.748,P＜0.01).결론:혈연치수평≥0.48μmol/L,가사외주혈CD3표체급CD4여CD8비치하강,대소인면역공능산생불량영향.
BACKGROUND: Although we have gained much information about leadinduced organ damage, the effect of blood lead level on T cell subgroup is yet to be determined.OBJECTIVE: To assess the effect of blood lead level on T cell subgroup of children, and the association of T cell subgroup with threshold limit value of blood lead level.DESIGN: Comparative observation.SETTING: The Institute of Molecular Microorganism, Maternity and Child Care Hospital, Shanxi Provincial Children Hospital.PARTICIPANTS: Sixty children, (32 boys and 28 girls), aged 3-6 years with a mean of (4.5±1.5) years old, were selected from the suburbs of Taiyuan city between May 2003 and October 2003. Informed consents were obtained from all their guardians. The enrolled children according to their blood lead levels were assigned into three groups, 13 in Group Ⅰ with blood lead level ≥ 0.48 μmol/L, 20 in Group 2 with lead level ≥ 0.24 μmol/L but ＜ 0.48 μmoL/L and 27 in Group 3 with lead level ＜ 0.24 μmol/L.METHODS: Blood lead level and expression of T cell subgroup were measured with graphite furnace atomic absorption spectroscopy and immunofluorescence methods respectively. Student t test was used in data analysis, and linear correlation analysis was used to assess the correlation between blood lead level and expression of T cell subgroup.level and expression of T cell subgroup.pression of CD3 and ratio of CD4 to CD8 were lower in Group 1 (lead level ≥0.48 μmol/L) than Group 3 (lead level ＜ 0.24 μmol/L) (t=3.27,blood lead level showed had significant inverse correlation with CD3 expression and the ratio of CD4 to CD8(r=-0.689,-0.674,P ＜ 0.01).CONCLUSION: A blood lead level ≥ 0.48 μmol/L, is shown to significantly decrease the expression of CD3 and ratio of CD4 to CD8 in peripheral blood, which may impair the children's immunological function.