中国化学工程学报（英文版）
中國化學工程學報（英文版）
중국화학공정학보（영문판）
CHINESE JOURNAL OF CHEMICAL ENGINEERING
2007年
4期
586-590
,共5页
秦海娜%修志龙%张代佳%包永明%李晓晖%韩国柱
秦海娜%脩誌龍%張代佳%包永明%李曉暉%韓國柱
진해나%수지룡%장대가%포영명%리효휘%한국주
PEGylated protein%hirudin%analysis%anti-thrombin activity
Hirudin is the most anticoagulant drug found in nature, but its short serum half-life significantly inhibits its clinical application. The PEGylation of hirudin, the most promising anticoagulant drug, was performed in this paper. The optimal reaction conditions for PEGylated hirudin were investigated. When the PEGylation reaction was conducted under 4℃ after 10h, in the borate buffer at pH 8.5, with the molar ratio 250: 1 of PEG to hirudin, a higher modification extent was achieved. Finally, the bioactivity of PEGylated hirudin was measured in vitro.Compared with unmodified hirudin, 26% of anti-thrombin activity was retained.
