CAJ | 학술논문

以绿僵菌(Nomuraea rileyi)和黑胸败血病菌(Bacillus sp.)为靶标,从泰山林地土壤中分离获得一株对多种家蚕病原菌具有强烈拮抗作用的放线菌BS-112.根据该菌株的形态特征、培养特征、理化性质、细胞壁组分及16S rDNA序列分析结果,将其鉴定为吸水链霉菌(Streptomyces hygroscopicus).该菌株抗菌谱广,抗菌物质耐高温,在酸、碱环境比较稳定,抗菌物质粗提物对黑胸败血病菌和绿僵菌的最小抑菌浓度分别为19.53 μg/mL和78.13 μg/mL,最小杀菌浓度分别为78.13 μg/mL 和156.25 μg/mL,表明在较低浓度下即可达到杀菌效果.
이록강균(Nomuraea rileyi)화흑흉패혈병균(Bacillus sp.)위파표,종태산임지토양중분리획득일주대다충가잠병원균구유강렬길항작용적방선균BS-112.근거해균주적형태특정、배양특정、이화성질、세포벽조분급16S rDNA서렬분석결과,장기감정위흡수련매균(Streptomyces hygroscopicus).해균주항균보엄,항균물질내고온,재산、감배경비교은정,항균물질조제물대흑흉패혈병균화록강균적최소억균농도분별위19.53 μg/mL화78.13 μg/mL,최소살균농도분별위78.13 μg/mL 화156.25 μg/mL,표명재교저농도하즉가체도살균효과.
Taking Nomuraea rileyi and Bacillus sp.as the target pathogens,an actinomyces strain BS-112 was isolated from soil in the forest land of Mountain Tai. This strain showed strong antagonistic activity against various silkworm pathogens. Based on its morphologic, culture, physiological and biochemical characteristics,and components of its cell wall as well as 16S rDNA sequence,BS-112 was identified as a Streptomyces hygroscopicus. It had a broad inhibition spectrum. The antifungal and antibacterial substances were resistant to high temperature and relatively stable under acidic or basic environment. The minimum inhibition concentration of extracellular crude antibacterial extracts against Bacillus sp.and N.rileyi was 19.53 and 78.13 μg/mL, respectively. And the minimum bactericidal concentration was 78.13 and 156.25 μg/mL, respectively. These results indicate that the obtained strain has satisfactory bactericidal effect under relatively low concen-tration.