CAJ | 학술논문

脂肪酸合酶(FAS)被认为是治疗肥胖症的潜在靶点.研究表明:油荼壳提取物对FAS具有很强的快结合可逆抑制作用.萃取的最佳溶剂为40%乙醇水溶液,此提取物的半抑制质量浓度为2.02μg/mL.而油茶饼70%乙醇提取物则具有很强的慢结合不可逆抑制作用,在0.31 mg/mL下2 min内FAS失活超过85%.结果表明:油荼壳提取物与油荼饼提取物分别在快结合抑制与慢结合抑制上显示出很强的抑制能力,预计为新的抑制剂,对研究FAS的作用机理与防治肥胖症的应用上可能具有重要的价值.
지방산합매(FAS)피인위시치료비반증적잠재파점.연구표명:유도각제취물대FAS구유흔강적쾌결합가역억제작용.췌취적최가용제위40%을순수용액,차제취물적반억제질량농도위2.02μg/mL.이유다병70%을순제취물칙구유흔강적만결합불가역억제작용,재0.31 mg/mL하2 min내FAS실활초과85%.결과표명:유도각제취물여유도병제취물분별재쾌결합억제여만결합억제상현시출흔강적억제능력,예계위신적억제제,대연구FAS적작용궤리여방치비반증적응용상가능구유중요적개치.
Fatty acid synthase (FAS) is reported as a potential new therapeutic target for obesity.However,there are limited known FAS inhibitors presently.Our study showed that the extract of oiltea shell (EOS) inhibited FAS activity with strong reversible fast -binding inhibition.40% ethanol was the optimal solvent for the extraction,and the half inhibition concentration ( IC_(50) ) of EOS to FAS overall reaction was 2.02μ g/mL.We also found the 70%ethanol extract of oiltea seed residue (EOSR) inhibited FAS activity with irreversible slow-binding inhibition.In the presence of 0.31 mg/mL of EOSR,the FAS activity inhibition was led to over 85% in 2 min.These results showed that FAS inhibitor from oiltea seed had stronger inhibition ability than the known inhibitors.It is expected to be a new effective FAS inhibitor,and may be of great value for the study on FAS mechanism and the control of obesity.