CAJ | 학술논문

目的比较改进的Bethesda方法与Nijmegen方法检测血浆凝血因子Ⅷ(FⅧ)抑制物的可靠性及实用性.方法自行改进Bethesda检测方法,将Nijmegen方法中的乏FⅧ血浆和Nijmegen正常混合血浆替换成缓冲液和通用参比血浆(UCRP)来制备标准曲线;在检测患者抑制物时将Nijmegen正常混合血浆替换成UCRP;以滴度≥0.6 BU/ml为抑制物阳性.分别采用改进Bethesda方法和Nijmegen方法对237例血友病A患者进行抑制物检测.结果改进Be-thesda方法和Nijmegen方法检测的阳性率分别为5.5%(13/237)和8.4%(20/237),两者相比差异无统计学意义(P>0.05).对于滴度>0.7 BU/ml的抑制物,两种方法具有很好的一致性.两种方法阳性结果不同的抑制物水平均在0.6～0.7 BU/ml.结论改进的标准Bethesda方法是一种简便、可行的检测方法,Nijmegen方法更有利于低滴度抑制物的检出.
목적 비교개진적Bethesda방법여Nijmegen방법검측혈장응혈인자Ⅷ(FⅧ)억제물적가고성급실용성.방법 자행개진Bethesda검측방법,장Nijmegen방법중적핍FⅧ혈장화Nijmegen정상혼합혈장체환성완충액화통용삼비혈장(UCRP)래제비표준곡선;재검측환자억제물시장Nijmegen정상혼합혈장체환성UCRP;이적도≥0.6 BU/ml위억제물양성.분별채용개진Bethesda방법화Nijmegen방법대237례혈우병A환자진행억제물검측.결과 개진Be-thesda방법화Nijmegen방법검측적양성솔분별위5.5%(13/237)화8.4%(20/237),량자상비차이무통계학의의(P>0.05).대우적도>0.7 BU/ml적억제물,량충방법구유흔호적일치성.량충방법양성결과불동적억제물수평균재0.6～0.7 BU/ml.결론 개진적표준Bethesda방법시일충간편、가행적검측방법,Nijmegen방법경유리우저적도억제물적검출.
Objective To compare the sensitivity and practicability of modified Bethesda assay and Nijmegen assay in detecting factor Ⅷ (FⅧ) inhibitor.Methods Modified Bethesda assay and Nijmegen assay were used to screen FⅧ inhibitors in 237 patients with hemophilia A.The buffer plus universal coagulation reference plasma (UCRP) was used to establish a standard curve for FⅧ: C assay in modified Bethesda method, instead of Nijmegen plasma plus FⅧ deficiency plasma in Nijmegen method.The cutoff value for positive FⅧ inhibitors is ≥0.6 BU/ml.Results The positive rate of FⅧ inhibitors was 5.5% (n = 13) when using modified Bethesda assay and was 8.4% (n = 20) when using Nijmegen assay (P > 0.05).Conclusion Modified standard Bethesda assay is a convenient and feasible method for detecting FⅧ inhibitors.