中华耳科学杂志
中華耳科學雜誌
중화이과학잡지
CHINESE JOURNAL OF OTOLOGY
2010年
1期
90-94
,共5页
田斐%李青峰%冀永进%马敏
田斐%李青峰%冀永進%馬敏
전비%리청봉%기영진%마민
胆脂瘤,中耳%破骨细胞分化因子%白介素-17A
膽脂瘤,中耳%破骨細胞分化因子%白介素-17A
담지류,중이%파골세포분화인자%백개소-17A
Cholesteatoma,middle ear%Receptor Activator of nuclear factor-kappa B ligand%Interleukin-17A
目的 观察破骨细胞分化因子(RANKL)和白介素-17A(IL-17A)在中耳胆脂瘤组织中的表达,探讨二者在中耳胆脂瘤骨质破坏机制中的作用.方法 采用免疫组织化学两步法和计算机图像定量分析法,检测RAN-KL、IL-17A在25例中耳胆脂瘤上皮、16例胆脂瘤周围肉芽组织及15例正常外耳道皮肤中的表达,同时观察二者的表达与临床听小骨骨质破坏程度的相互关系.结果 (1)RANKL、IL-17A在胆脂瘤上皮及胆脂瘤周围肉芽组织中表达增高,分别与正常对照组比较,差异均有统计学意义(t_(RANKL)=9.864、5.630,均P<0.05;t_(IL-17A)=13.905、9.011.均P<0.05),且二者的表达与骨质破坏程度相关.(2)在胆脂瘤上皮及胆脂瘤周围肉芽组织中,RANKL与IL-17A的表达呈正相关(r_(上皮)=0.692,r_(肉芽)=0.538,P<0.05).结论 RANKL及IL-17A在中耳胆脂瘤组织中呈高表达,与骨质破坏程度成正比,表明其与胆脂瘤骨质破坏机制密切相关;RANKL与IL-17A表达有相关性,提示二者之间可能存在相互作用.
目的 觀察破骨細胞分化因子(RANKL)和白介素-17A(IL-17A)在中耳膽脂瘤組織中的錶達,探討二者在中耳膽脂瘤骨質破壞機製中的作用.方法 採用免疫組織化學兩步法和計算機圖像定量分析法,檢測RAN-KL、IL-17A在25例中耳膽脂瘤上皮、16例膽脂瘤週圍肉芽組織及15例正常外耳道皮膚中的錶達,同時觀察二者的錶達與臨床聽小骨骨質破壞程度的相互關繫.結果 (1)RANKL、IL-17A在膽脂瘤上皮及膽脂瘤週圍肉芽組織中錶達增高,分彆與正常對照組比較,差異均有統計學意義(t_(RANKL)=9.864、5.630,均P<0.05;t_(IL-17A)=13.905、9.011.均P<0.05),且二者的錶達與骨質破壞程度相關.(2)在膽脂瘤上皮及膽脂瘤週圍肉芽組織中,RANKL與IL-17A的錶達呈正相關(r_(上皮)=0.692,r_(肉芽)=0.538,P<0.05).結論 RANKL及IL-17A在中耳膽脂瘤組織中呈高錶達,與骨質破壞程度成正比,錶明其與膽脂瘤骨質破壞機製密切相關;RANKL與IL-17A錶達有相關性,提示二者之間可能存在相互作用.
목적 관찰파골세포분화인자(RANKL)화백개소-17A(IL-17A)재중이담지류조직중적표체,탐토이자재중이담지류골질파배궤제중적작용.방법 채용면역조직화학량보법화계산궤도상정량분석법,검측RAN-KL、IL-17A재25례중이담지류상피、16례담지류주위육아조직급15례정상외이도피부중적표체,동시관찰이자적표체여림상은소골골질파배정도적상호관계.결과 (1)RANKL、IL-17A재담지류상피급담지류주위육아조직중표체증고,분별여정상대조조비교,차이균유통계학의의(t_(RANKL)=9.864、5.630,균P<0.05;t_(IL-17A)=13.905、9.011.균P<0.05),차이자적표체여골질파배정도상관.(2)재담지류상피급담지류주위육아조직중,RANKL여IL-17A적표체정정상관(r_(상피)=0.692,r_(육아)=0.538,P<0.05).결론 RANKL급IL-17A재중이담지류조직중정고표체,여골질파배정도성정비,표명기여담지류골질파배궤제밀절상관;RANKL여IL-17A표체유상관성,제시이자지간가능존재상호작용.
Objective To study expression of RANKL (Receptor Activator of Nuclear Factor-kappa B Ligand) and IL -17A (Interleukin -17A) in Human Middle Ear Cholesteatoma and their roles in bone destruction. Methods Immunohistochemical methods and computer-aided quantitative image analysis were used to examine the expression of RANKL and IL-17A in 25 middle ear cholesteatoma specimens, 16 granulation tissue surrounding the cholesteatoma and 15 specimens of normal external ear canal skin. The correlation between expression of the two indexes and the erosion of ossicles were also examined. Results (1) Increased rexpression of the cytokine RANKL and IL-17A were found in the cholesteatoma epithelium and surrounding granulation tissue, as compared with normal external meatal skin(t_(RANKL) = 9.864, 5.630, P<0.05; t_(IL-17A)= 13.905, 9.011, P<0.05). In addition, expression of the two indexes were correlated to the extent of ossicle destruction. (2) In cholesteatoma epithelium and granulation tissue, expression of RANKL was directly correlated with that of IL-17A (P < 0.05). Conclusion RANKL and IL-17A are overexpressed in middle ear cholesteatoma and they are associated with ossicle destruction, indicating that RANKL and IL-17A are responsible for bone destruction in cholesteatoma; RANKL and IL-17A have a correlation in their expression, suggesting that the two factors may interact with each other.
