CAJ | 학술논문

目的:探讨蚝菇提取物对老年大鼠(24月龄)肝肾组织内过氧化物酶基因表达及蛋白质羰基含量的影响.方法:18只大鼠被分为3组,每组6只.组1为健康年轻大鼠(4月龄)对照组;组2为健康老年大鼠(24月龄)对照组;组3为健康老年大鼠(24月龄)蚝菇提取物干预组,干预措施为每天腹腔注射蚝菇提取物200 mg/kg体质量,连续注射30 d.第31天处死全部大鼠并取出肝肾组织.使用逆转录聚合酶链反应及分光光度测定法分别测定大鼠肝肾组织内过氧化物酶基因表达情况及蛋白质羰基的含量.结果:组3老年大鼠注射蚝菇提取物后,其肝肾组织内过氧化物酶基因表达显著高于组2老年大鼠,且组3老年大鼠肝肾组织内蛋白羰基含量显著低于组2老年大鼠.结论:蚝菇提取物能够增加老年大鼠肝肾组织内抗氧化酶基因的表达并减少自由基引起的蛋白质氧化,对于自由基引起的年龄相关性疾病可能有保护作用.
목적:탐토자고제취물대노년대서(24월령)간신조직내과양화물매기인표체급단백질탄기함량적영향.방법:18지대서피분위3조,매조6지.조1위건강년경대서(4월령)대조조;조2위건강노년대서(24월령)대조조;조3위건강노년대서(24월령)자고제취물간예조,간예조시위매천복강주사자고제취물200 mg/kg체질량,련속주사30 d.제31천처사전부대서병취출간신조직.사용역전록취합매련반응급분광광도측정법분별측정대서간신조직내과양화물매기인표체정황급단백질탄기적함량.결과:조3노년대서주사자고제취물후,기간신조직내과양화물매기인표체현저고우조2노년대서,차조3노년대서간신조직내단백탄기함량현저저우조2노년대서.결론:자고제취물능구증가노년대서간신조직내항양화매기인적표체병감소자유기인기적단백질양화,대우자유기인기적년령상관성질병가능유보호작용.
Objective: The objective of the present study was to address the effect of mushroom Pleurotus ostreatus on the catalase(CAT)gene expression and the protein carbonyls in liver and kidney of aged(24 months old)rats.Methods: Eighteen acclimated rats were divided into 3 groups of 6 each: group Ⅰ,normal young(4 months old)rats; group Ⅱ,normal aged(24 months old)untreated rats; group Ⅲ,normal aged rats treated with mushroom P.ostreatus extract(200 mg/kg body weight administered intraperitoneally for 30 days).On the 31st day,rats were sacrificed by decapitation; the livers and kidneys were removed,washed free of blood,blotted dry and processed immediately.Reverse transcriptase-polymerase chain reaction(RT-PCR)and spectrophotometry were utilized for the analyses of CAT gene expression and protein carbonyl content in the tissues of livers and kidneys.Results: In aged rats that had been treated with the extract of P.ostreatus(group Ⅲ),the level of the transcript of CAT gene was found to be higher than that in liver(P<0.01)and kidney(P<0.05)of aged untreated (group Ⅱ)rats,respectively.Treatment of aged rats with P.ostreatus extract(group Ⅲ)resulted in protein carbonyt levels being significantly lower in liver(P<0.05)and kidney(P<0.01)than those observed in aged untreated(group Ⅱ)rats.Conclusion: These results suggest that an extract of P.ostreatus can enhance the antioxidant enzyme(CAT)gene expression and could decrease the incidence of free radical-induced protein oxidation in aged rats,thereby protecting the occurrence of age-associated disorders that involve free radicals.