国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2011年
4期
307-310
,共4页
桥本氏甲状腺炎%IL-17%Foxp3%IL-10
橋本氏甲狀腺炎%IL-17%Foxp3%IL-10
교본씨갑상선염%IL-17%Foxp3%IL-10
Hashimoto' s thyroiditis%IL-17%Foxp3%IL-10
目的 检测桥本氏甲状腺炎(HT)患者甲状腺组织及外周静脉血中Th17细胞和调节性T 细胞(Treg)细胞相关细胞因子的表达水平,分析Th17/Treg免疫平衡在HT发病机制中的作用.方法 采用逆转录-聚合酶链反应(RT-PCR)法,检测10例HT甲功正常组、10例HT甲低组和10例正常甲状腺组织(对照组)中IL-17A、Foxp3mRNA的表达水平;另采用ELISA法,检测上述三组血清中IL-17、IL-10的含量.结果 与对照组相比,HT患者甲状腺组织内IL-17AmRNA的表达显著升高(t=0.5061,P<0.05),且HT甲低组高于HT甲功正常组(t=0.2575,P<0.05);HT患者甲状腺组织中Foxp3mRNA的表达降低(t=O.5298,P<0.05),但其表达水平则与甲状腺功能无关.HT患者血清IL-17水平明显高于对照组(t=5.5840,P<0.05),且HT甲低组高于HT甲功正常组(t=2.2760,P<0.05);HT患者血清IL-10水平明显低于对照组(t=17.9170,P<0.05),但HT甲低组与HT甲功正常组则差异无统计学意义(t=O.0410,P>0.05).结论 HT患者甲状腺组织和外周血中Th17细胞应答增强,而Treg细胞免疫抑制下降;Th17/Treg免疫失衡可能参与HT的发生发展.
目的 檢測橋本氏甲狀腺炎(HT)患者甲狀腺組織及外週靜脈血中Th17細胞和調節性T 細胞(Treg)細胞相關細胞因子的錶達水平,分析Th17/Treg免疫平衡在HT髮病機製中的作用.方法 採用逆轉錄-聚閤酶鏈反應(RT-PCR)法,檢測10例HT甲功正常組、10例HT甲低組和10例正常甲狀腺組織(對照組)中IL-17A、Foxp3mRNA的錶達水平;另採用ELISA法,檢測上述三組血清中IL-17、IL-10的含量.結果 與對照組相比,HT患者甲狀腺組織內IL-17AmRNA的錶達顯著升高(t=0.5061,P<0.05),且HT甲低組高于HT甲功正常組(t=0.2575,P<0.05);HT患者甲狀腺組織中Foxp3mRNA的錶達降低(t=O.5298,P<0.05),但其錶達水平則與甲狀腺功能無關.HT患者血清IL-17水平明顯高于對照組(t=5.5840,P<0.05),且HT甲低組高于HT甲功正常組(t=2.2760,P<0.05);HT患者血清IL-10水平明顯低于對照組(t=17.9170,P<0.05),但HT甲低組與HT甲功正常組則差異無統計學意義(t=O.0410,P>0.05).結論 HT患者甲狀腺組織和外週血中Th17細胞應答增彊,而Treg細胞免疫抑製下降;Th17/Treg免疫失衡可能參與HT的髮生髮展.
목적 검측교본씨갑상선염(HT)환자갑상선조직급외주정맥혈중Th17세포화조절성T 세포(Treg)세포상관세포인자적표체수평,분석Th17/Treg면역평형재HT발병궤제중적작용.방법 채용역전록-취합매련반응(RT-PCR)법,검측10례HT갑공정상조、10례HT갑저조화10례정상갑상선조직(대조조)중IL-17A、Foxp3mRNA적표체수평;령채용ELISA법,검측상술삼조혈청중IL-17、IL-10적함량.결과 여대조조상비,HT환자갑상선조직내IL-17AmRNA적표체현저승고(t=0.5061,P<0.05),차HT갑저조고우HT갑공정상조(t=0.2575,P<0.05);HT환자갑상선조직중Foxp3mRNA적표체강저(t=O.5298,P<0.05),단기표체수평칙여갑상선공능무관.HT환자혈청IL-17수평명현고우대조조(t=5.5840,P<0.05),차HT갑저조고우HT갑공정상조(t=2.2760,P<0.05);HT환자혈청IL-10수평명현저우대조조(t=17.9170,P<0.05),단HT갑저조여HT갑공정상조칙차이무통계학의의(t=O.0410,P>0.05).결론 HT환자갑상선조직화외주혈중Th17세포응답증강,이Treg세포면역억제하강;Th17/Treg면역실형가능삼여HT적발생발전.
Objective To observe the expression of related cytokines of Thl7 and CD4+ CD25+ regulatory T cells in the thyroid and peripheral blood of patients with Hashimoto' s thyroiditis,and to explore the role of Thl7/Treg balance in the pathogenesis of HT. Methods Thyroid and peripheral blood were collected from 10 patients with HT who developed hypothyroidism,10 patients with HT who were euthyroid and 10 control subjects without HT(control group). The expression of IL-17A and Foxp3 mRNA in thyroid were measured by RT-PCR. The levels of related cytokines IL-17and IL-10 in peripheral blood were assayed by ELISA. Results Compared with control group, in patients with HT, the expression of IL-17A mRNA in thyroid increased significantly (t=0.5061 ,P<0.05),which increased markedly in hypothyroidism group than that in euthyroid group(t =0.2575,P<0. 05) ; the expression of Foxp3 mRNA in thyroid decreased significantly in HT patients(t=0.5298, P<0.05) , but it seemed to have nothing to do with thyroid function state. The serum levels of IL-17 in patients with HT increased significantly(t =5.5840,P<0.05),which were higher in hypothyroidism group than that in euthyroid group(t=2.2760, P<0.05); the serum levels of IL-10 decreased significantly in HT patinents (t=17.9170,P<0.05) ,but there were no significant difference between hypothyroidism group and euthyroid group(t=0.0410,P>0.05). Conclusion The increased response of Thl7 cells and diminished immunosup-pression of Treg cells are presented both thyroid and peripheral blood in patients with HT, suggesting that the imbalance between Th17 and Treg may play an important role in the process of HT.
