CAJ | 학술논문

目的采用高血糖条件下Sprague-Dawley(SD)大鼠局灶性脑缺血再灌注模型,观察神经功能缺损评分、脑梗死体积、脑组织病理形态改变及抑凋亡基因bcl-2的表达情况,探讨预防性应用盐酸氟桂利嗪对高血糖条件下SD大鼠局灶性脑缺血再灌注损伤是否有保护作用.方法 36只雄性健康SD大鼠,建立高血糖模型后随机分为2组:高血糖组(n=18)、盐酸氟桂利嗪+高血糖组(简称氟桂利嗪组n=18),各组按脑缺血90 min再灌注3 h(n=6)、6 h(n=6)、24 h(n=6)分为3个亚组.比较氟桂利嗪组与高血糖组各再灌注时间点神经功能缺损评分、脑梗死体积和脑组织病理形态的改变.结果相同再灌注时间点,氟桂利嗪组比高血糖组神经功能缺损程度减轻,P＜0.05;相同时间点氟桂利嗪组较高血糖组梗死体积缩小,其中再灌注3、6 h组间比较P＜0.05,再灌注24 h组间比较P＜0.01;脑组织病理形态观察:氟桂利嗪组与高血糖组各再灌注时间点比较,变性、坏死的神经元减少,空泡化改变减轻,组织间水肿减轻.结论预防性应用盐酸氟桂利嗪能减轻高血精条件下的局灶性脑缺血再灌注损伤,减轻神经功能缺损症状,缩小梗死体积,减轻神经细胞变性、坏死及组织水肿.
목적 채용고혈당조건하Sprague-Dawley(SD)대서국조성뇌결혈재관주모형,관찰신경공능결손평분、뇌경사체적、뇌조직병리형태개변급억조망기인bcl-2적표체정황,탐토예방성응용염산불계리진대고혈당조건하SD대서국조성뇌결혈재관주손상시부유보호작용.방법 36지웅성건강SD대서,건립고혈당모형후수궤분위2조:고혈당조(n=18)、염산불계리진+고혈당조(간칭불계리진조n=18),각조안뇌결혈90 min재관주3 h(n=6)、6 h(n=6)、24 h(n=6)분위3개아조.비교불계리진조여고혈당조각재관주시간점신경공능결손평분、뇌경사체적화뇌조직병리형태적개변.결과 상동재관주시간점,불계리진조비고혈당조신경공능결손정도감경,P＜0.05;상동시간점불계리진조교고혈당조경사체적축소,기중재관주3、6 h조간비교P＜0.05,재관주24 h조간비교P＜0.01;뇌조직병리형태관찰:불계리진조여고혈당조각재관주시간점비교,변성、배사적신경원감소,공포화개변감경,조직간수종감경.결론 예방성응용염산불계리진능감경고혈정조건하적국조성뇌결혈재관주손상,감경신경공능결손증상,축소경사체적,감경신경세포변성、배사급조직수종.
Objective Applying focal ischemia-reperfusion model of SD rat on the condition of hyperglycemia, through observing the state of neurologic impairment score, cerebral pathomorphology and infarction volume after focal ischemia-reperfusion damage in SD rat on the condition of hyperglycemia. Method :36 healthy male SD rats(weight from 180 g to 220 g) with normal blood glucemia( ＜6 mmol/l)were randomly divided into 2 groups : hyperglycemia group ( n = 18 ) and flunarizine + hyperglycemia group ( namely flunarizine group n =18 ). Each group was divided into 3 subgroups according to reperfusion 3 h ( n = 6 ), 6 h ( n = 6), 24 h ( n = 6 ) after ischemia for 90 minutes. Compare the differences of neurologic impairment score, cerebral infarction volume and pathomorphology. Results 1. There are great difference of the neurologic impairment score between the flunarizine group and hyperglycemia group(P ＜0. 05) ,The infarction volume can be seen at flunarizine group,and the peak was at reperfusion 24 hours. Compared 6 h with 3 h and 24 h with 6 h in flunarizine group, P ＜0. 01. The infarction volume in the flunarizine group was lower than that in the hyperglycemia group,P ＜0. 01 at reperfusion 3 hours and 6 hours, P ＜ 0. 05 at reperfusion 24 hours. Cerebral tissue pathomorphology:In flunarizine group, the injury of cerebral tissue became serious with time went by, but compared with hyperglycemia group, the number of neuron which became degeneration and necrosis decreased, vacuolization and intertissue edema became relieved. Conclusion On the condition of hyperglycemia, pretreatment of flunarizine could decrease focal ischemia-reperfusion damage, relieve the neurologic impairment symptoms; reduce infarction volume, reduce the neuron degeneration, necrosis and tissue edma.