CAJ | 학술논문

目的评价不同剂量利多卡因对脓毒症大鼠急性肝损伤的影响.方法健康清洁级雄性Wistar大鼠50只,体重200 ～ 250 g,8-10周龄,采用随机数字表法将大鼠随机分为5组(n=10):假手术组(S组)、模型组(CLP组)、不同剂量利多卡因组(L1-3组).采用盲肠结扎穿孔(CLP)法制备脓毒症模型.于CLP术后即刻、1、2h时S组和CLP组分别腹腔注射生理盐水0.5 ml,L1-2组分别腹腔注射利多卡因5、10和20 mg/kg.于术后24h时采集血样测定血浆谷丙转氨酶(ALT)活性,处死后取肝组织检测高迁移率族蛋白1(HMGB1)mRNA表达水平,光镜下观察肝组织病理学改变.结果与S组比较,其余各组血浆ALT浓度升高,肝组织HMGBl mRNA表达上调(P＜0.05).与CLP组比较,L 1-3组肝组织HMGB1 mRNA表达下调,L2组和L3组血浆ALT浓度降低(P＜0.05).与L1组比较L2组和L3组血浆ALT浓度降低,肝组织HMGB1 mRNA表达下调(P＜0.05).与L2组比较L3组血浆ALT浓度降低,肝组织HMGB1 mRNA表达下调(P＜0.05).CLP组肝组织病理损伤严重,L1-3组肝组织病理损伤均减轻,且L3组最为明显.结论利多卡因可减轻脓毒症大鼠急性肝损伤,且与剂最有关,其机制与抑制肝组织HMGB1 mRNA过表达有关.
목적 평개불동제량리다잡인대농독증대서급성간손상적영향.방법 건강청길급웅성Wistar대서50지,체중200 ～ 250 g,8-10주령,채용수궤수자표법장대서수궤분위5조(n=10):가수술조(S조)、모형조(CLP조)、불동제량리다잡인조(L1-3조).채용맹장결찰천공(CLP)법제비농독증모형.우CLP술후즉각、1、2h시S조화CLP조분별복강주사생리염수0.5 ml,L1-2조분별복강주사리다잡인5、10화20 mg/kg.우술후24h시채집혈양측정혈장곡병전안매(ALT)활성,처사후취간조직검측고천이솔족단백1(HMGB1)mRNA표체수평,광경하관찰간조직병이학개변.결과 여S조비교,기여각조혈장ALT농도승고,간조직HMGBl mRNA표체상조(P＜0.05).여CLP조비교,L 1-3조간조직HMGB1 mRNA표체하조,L2조화L3조혈장ALT농도강저(P＜0.05).여L1조비교L2조화L3조혈장ALT농도강저,간조직HMGB1 mRNA표체하조(P＜0.05).여L2조비교L3조혈장ALT농도강저,간조직HMGB1 mRNA표체하조(P＜0.05).CLP조간조직병리손상엄중,L1-3조간조직병리손상균감경,차L3조최위명현.결론 리다잡인가감경농독증대서급성간손상,차여제최유관,기궤제여억제간조직HMGB1 mRNA과표체유관.
Objective To investigate the effects of different doses of lidocaine on acute liver injury in septic rats.Methods Fifty male Wistar rats,weighing 200-250 g,aged 8-10 weeks,were randomly divided into 5 groups(n =10 each):sham operation group(group S),sepsis group(group CLP),and different doses of lidocaine groups(groups L1-3).Sepsis was induced by cecal ligation and puncture(CLP)in anesthetized rats.At 0,1 and 2 h after CLP,lidocaine 5,10 and 20 mg/kg(in normal saline 0.5 ml)were injected intraperitoneally in groups L1-3 respectively,while normal saline 0.5 ml was given in groups S and CLP.At 24 h after CLP,blood samples were taken for determination of the plasma alanine aminotran sferase(ALT)concenlralion.The rats were then sacrificed,and the liver was removed for microscopic examination and determination of the hepatic high-mobility group box 1 protein(HMGBI)mRNA expression.Results Compared with group S,the plasma ALT concentration was significandy increased and hepatic HMGBI mRNA expression was up-regulated in groups CLP and L1-3(P ＜ 0.05).Compared with group CLP,HMGBI mRNA expression was down-regulated in groups 14-3,while the plasma ALT concentration was decreased in groups L2 and L3(P ＜ 0.05),The plasma ALT concentration was significantly decreased and HMGBI mRNA expression was down-regulated in groups L2 and L3 com pared with group L1,and in group L3 compared with group L2(P ＜ 0.05).The microscopic examination showed that the pathologic changes were attenuated in groups L1-3,and the changes were least severe in group L3.Concluslon Lidocaine can reduce acute liver injury in septic rats,this effect is dose-related,and inhibition of hepatic HMGBI mRNA expression is involved in the mechanism.