CAJ | 학술논문

目的研究微波暴露对大鼠海马脑区热休克蛋白(HSP)70表达的变化,为阐明微波的生物效应与机制提供线索.方法采用峰值功率密度为90、5 W/cm2的微波全身一次性辐照大鼠20min,采用反转录-聚合酶链反应(RT-PCR)法观察微波辐照后不同时相点大鼠海马脑区hsp70 mRNA表达的变化;采用免疫印迹(Western-blot)法观察微波辐照后HSP70蛋白水平的变化.结果 90、5W/cm2微波辐照后,大鼠的肛温[分别为(40.40±0.19)、(38.22±0.68)℃]以及比吸收率(SAR)值[分别为(15.09±0.81)、(5.56±0.31)W/kg]明显升高.2个微波暴露组在20 min暴露后均可见hsp70 mRNA和蛋白水平表达上调.结论微波辐照有明显的热效应,是hsp70合成极为敏感的诱因,并可能启动了脑的内源性保护机制.
목적 연구미파폭로대대서해마뇌구열휴극단백(HSP)70표체적변화,위천명미파적생물효응여궤제제공선색.방법 채용봉치공솔밀도위90、5 W/cm2적미파전신일차성복조대서20min,채용반전록-취합매련반응(RT-PCR)법관찰미파복조후불동시상점대서해마뇌구hsp70 mRNA표체적변화;채용면역인적(Western-blot)법관찰미파복조후HSP70단백수평적변화.결과 90、5W/cm2미파복조후,대서적항온[분별위(40.40±0.19)、(38.22±0.68)℃]이급비흡수솔(SAR)치[분별위(15.09±0.81)、(5.56±0.31)W/kg]명현승고.2개미파폭로조재20 min폭로후균가견hsp70 mRNA화단백수평표체상조.결론 미파복조유명현적열효응,시hsp70합성겁위민감적유인,병가능계동료뇌적내원성보호궤제.
Objective To study the change of heat shock protein(HSP)70 expression after exposure to occupational microwave in rats hippocampus, and explore the role of HSP70 in the mechanism of bio-effect of microwave irradiation. Methods The animal model was established by whole body exposures in 90, 5 W/cm2 microwave irradiation field for 20 min in rats. Changes of the mRNA of hsp70 expressions in rat hippocampus at different time were studied by RT-PCR, and the protein change by Western blot. Results The mRNA and protein expression of hsp70 in rat hippocampus increased after 90 W/cm2 and 5 W/cm2 microwave irradiation for 20 min. The anal temperature and the value of SAR increased significantly. These changes were positively correlated with power and irradiation time of microwave. The results indicated that microwave irradiation led to HSP70 syntheses effectively. Conclusion Microwave irradiation can obviously induce the thermal effect and activate HSP70, and initiate the endogens protective mechanism of central nervous system.