国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2009年
4期
197-200
,共4页
李志奎%李树钧%宋立强%张艰%遆新宇%赵峰%任新玲%陈卫强%王长征%钱桂生
李誌奎%李樹鈞%宋立彊%張艱%遆新宇%趙峰%任新玲%陳衛彊%王長徵%錢桂生
리지규%리수균%송립강%장간%제신우%조봉%임신령%진위강%왕장정%전계생
哮喘%嗜酸粒细胞%凋亡%共同β受体
哮喘%嗜痠粒細胞%凋亡%共同β受體
효천%기산립세포%조망%공동β수체
Asthma%Eosinophils%Apoptosis%Common β receptor
目的 观察地塞米松对哮喘豚鼠体外不同密度嗜酸粒细胞(EOS)凋亡及IL-3、IL-5和粒细胞-巨噬细胞集落刺激因子受体的共同β受体(βcR)mRNA表达的影响.探讨地塞米松促进哮喘EOS凋亡的机制.方法 卵蛋白激发哮喘豚鼠动物模型48 h后行支气管肺泡灌洗,分离低密度EOS(HEOS)及正常密度EOS(NEOS).HEOS及NEOS分别与地塞米松(10-10～10-5mol/L)共培养24 h,以原位杂交方法检测不同密度EOS的βcR mRNA表达,3'末端脱氧核苷转移酶介导的脱氧三磷酸尿苷缺口末端标记法检测细胞凋亡.结果 地塞米松干预24 h后,可见EOS凋亡增加的同时,不同密度EOS中βcR mRNA表达下降,并与地塞米松浓度呈剂量依赖性.βcR表达与EOS凋亡呈负相关(P<0.05).结论 地塞米松可抑制不同密度EOS表达βcR,抑制其细胞因子活动,促进EOS凋亡.地塞米松可以通过增加EOS中βcR表达调节EOS凋亡.
目的 觀察地塞米鬆對哮喘豚鼠體外不同密度嗜痠粒細胞(EOS)凋亡及IL-3、IL-5和粒細胞-巨噬細胞集落刺激因子受體的共同β受體(βcR)mRNA錶達的影響.探討地塞米鬆促進哮喘EOS凋亡的機製.方法 卵蛋白激髮哮喘豚鼠動物模型48 h後行支氣管肺泡灌洗,分離低密度EOS(HEOS)及正常密度EOS(NEOS).HEOS及NEOS分彆與地塞米鬆(10-10～10-5mol/L)共培養24 h,以原位雜交方法檢測不同密度EOS的βcR mRNA錶達,3'末耑脫氧覈苷轉移酶介導的脫氧三燐痠尿苷缺口末耑標記法檢測細胞凋亡.結果 地塞米鬆榦預24 h後,可見EOS凋亡增加的同時,不同密度EOS中βcR mRNA錶達下降,併與地塞米鬆濃度呈劑量依賴性.βcR錶達與EOS凋亡呈負相關(P<0.05).結論 地塞米鬆可抑製不同密度EOS錶達βcR,抑製其細胞因子活動,促進EOS凋亡.地塞米鬆可以通過增加EOS中βcR錶達調節EOS凋亡.
목적 관찰지새미송대효천돈서체외불동밀도기산립세포(EOS)조망급IL-3、IL-5화립세포-거서세포집락자격인자수체적공동β수체(βcR)mRNA표체적영향.탐토지새미송촉진효천EOS조망적궤제.방법 란단백격발효천돈서동물모형48 h후행지기관폐포관세,분리저밀도EOS(HEOS)급정상밀도EOS(NEOS).HEOS급NEOS분별여지새미송(10-10～10-5mol/L)공배양24 h,이원위잡교방법검측불동밀도EOS적βcR mRNA표체,3'말단탈양핵감전이매개도적탈양삼린산뇨감결구말단표기법검측세포조망.결과 지새미송간예24 h후,가견EOS조망증가적동시,불동밀도EOS중βcR mRNA표체하강,병여지새미송농도정제량의뢰성.βcR표체여EOS조망정부상관(P<0.05).결론 지새미송가억제불동밀도EOS표체βcR,억제기세포인자활동,촉진EOS조망.지새미송가이통과증가EOS중βcR표체조절EOS조망.
Objective To investigate the effects of dexamethasone on expression of common β receptor (βcR) mRNA of interleukin(IL)-3, IL-5, GM-CSF receptors and apoptosis in eosinophils (EOS) of bronchoalveolar lavage fluid(BALF) in asthmatic guinea pig, and mechanism of dexamethasone promoting EOS apoptosis. Methods After guineas pigs were stimulated by ovalbumin for 48 hours,hypodense EOS and normodense EOS were purified from BALF by gradients of percoll. EOS were cultured in PRMI1640 medium. Dexamethasone (10-10-10-5 mol/L) was added in the wells. The mRNA expression of βcR in EOS was measured by hybridization. Apoptosis was detected by TdT-mediated dUTP nick end labeling. Results EOS cultured under the condition of dexamethasone administration in vitro, apoptotic EOS increased, βcR mRNA expression decreased in a dose-dependent manner. There was significant negative correlation between EOS apoptosis and βcR mRNA expression. Conclusions Dexamethasone promotes EOS apoptosis,decreases expression of βcR in dose-dependent manner in vitro. βcR might be one of mechanisms that dexamethasone promotes apoptosis of EOS.
