中华创伤骨科杂志
中華創傷骨科雜誌
중화창상골과잡지
CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA
2011年
5期
437-442
,共6页
刘善宇%王钢%李岗%贾谊
劉善宇%王鋼%李崗%賈誼
류선우%왕강%리강%가의
生物相容性材料%股骨%材料试验%兔%骨缺损
生物相容性材料%股骨%材料試驗%兔%骨缺損
생물상용성재료%고골%재료시험%토%골결손
Biocompatible materials%Femur%Materials testing%Ribbits%Bone defect
目的 探讨新型多孔块状可吸收生物活性玻璃骨替代材料的骨缺损修复能力和生物相容性.方法选取30只健康成年新西兰大白兔,建立舣侧股骨髁直径为6 mm、深10～12 mm的骨缺损模型,根据骨缺损中植入的材料不同随机分为3组,每组10只20侧:实验组(A组)植人多孔块状可吸收生物活性玻璃骨替代材料,材料对照组(B组)植入常用非多孔生物活性玻璃骨替代材料,空白对照组(C组)不植入任何材料.术后6、12周取材,通过大体观察、X线片、显微CT和病理组织切片检测评价新型多孔块状可吸收生物活性玻璃骨替代材料的成骨能力和生物降解性能.结果植入后6周A、B组可见新生骨逐渐向材料内部生长,A组新生骨的数量明显多于B组.12周时A组植入材料几乎完全降解,骨缺损完全修复,成熟的骨小梁塑形改造;B组植骨中心区仍有较多材料尚未完全降解;C组骨缺损末愈合.组织切片定量分析显示:6周时A、B、C组新生骨占骨缺损的面积比平均分别为0.30±0.02、0.17±0.03、0.06±0.01,差异有统计学意义(F=374.202,P<0.001),组问两两比较差异均有统计学意义(P<0.05);12周时A、B、C组新生骨占骨缺损的面积比平均分别为0.53 ±0.05、0.39±0.05、0.07±0.01,差异有统计学意义(F=317.243,P<0.001),组间两两比较差异均有统计学意义(P<0.05).A组与B组6、12周时残留材料占骨缺损面积比差异均有统计学意义(P<0.05).结论新型多孔块状可吸收生物活性玻璃骨替代材料因具有良好的三维结构而表现出更好的成骨能力和生物降解性能.
目的 探討新型多孔塊狀可吸收生物活性玻璃骨替代材料的骨缺損脩複能力和生物相容性.方法選取30隻健康成年新西蘭大白兔,建立艤側股骨髁直徑為6 mm、深10～12 mm的骨缺損模型,根據骨缺損中植入的材料不同隨機分為3組,每組10隻20側:實驗組(A組)植人多孔塊狀可吸收生物活性玻璃骨替代材料,材料對照組(B組)植入常用非多孔生物活性玻璃骨替代材料,空白對照組(C組)不植入任何材料.術後6、12週取材,通過大體觀察、X線片、顯微CT和病理組織切片檢測評價新型多孔塊狀可吸收生物活性玻璃骨替代材料的成骨能力和生物降解性能.結果植入後6週A、B組可見新生骨逐漸嚮材料內部生長,A組新生骨的數量明顯多于B組.12週時A組植入材料幾乎完全降解,骨缺損完全脩複,成熟的骨小樑塑形改造;B組植骨中心區仍有較多材料尚未完全降解;C組骨缺損末愈閤.組織切片定量分析顯示:6週時A、B、C組新生骨佔骨缺損的麵積比平均分彆為0.30±0.02、0.17±0.03、0.06±0.01,差異有統計學意義(F=374.202,P<0.001),組問兩兩比較差異均有統計學意義(P<0.05);12週時A、B、C組新生骨佔骨缺損的麵積比平均分彆為0.53 ±0.05、0.39±0.05、0.07±0.01,差異有統計學意義(F=317.243,P<0.001),組間兩兩比較差異均有統計學意義(P<0.05).A組與B組6、12週時殘留材料佔骨缺損麵積比差異均有統計學意義(P<0.05).結論新型多孔塊狀可吸收生物活性玻璃骨替代材料因具有良好的三維結構而錶現齣更好的成骨能力和生物降解性能.
목적 탐토신형다공괴상가흡수생물활성파리골체대재료적골결손수복능력화생물상용성.방법선취30지건강성년신서란대백토,건립의측고골과직경위6 mm、심10～12 mm적골결손모형,근거골결손중식입적재료불동수궤분위3조,매조10지20측:실험조(A조)식인다공괴상가흡수생물활성파리골체대재료,재료대조조(B조)식입상용비다공생물활성파리골체대재료,공백대조조(C조)불식입임하재료.술후6、12주취재,통과대체관찰、X선편、현미CT화병리조직절편검측평개신형다공괴상가흡수생물활성파리골체대재료적성골능력화생물강해성능.결과식입후6주A、B조가견신생골축점향재료내부생장,A조신생골적수량명현다우B조.12주시A조식입재료궤호완전강해,골결손완전수복,성숙적골소량소형개조;B조식골중심구잉유교다재료상미완전강해;C조골결손말유합.조직절편정량분석현시:6주시A、B、C조신생골점골결손적면적비평균분별위0.30±0.02、0.17±0.03、0.06±0.01,차이유통계학의의(F=374.202,P<0.001),조문량량비교차이균유통계학의의(P<0.05);12주시A、B、C조신생골점골결손적면적비평균분별위0.53 ±0.05、0.39±0.05、0.07±0.01,차이유통계학의의(F=317.243,P<0.001),조간량량비교차이균유통계학의의(P<0.05).A조여B조6、12주시잔류재료점골결손면적비차이균유통계학의의(P<0.05).결론신형다공괴상가흡수생물활성파리골체대재료인구유량호적삼유결구이표현출경호적성골능력화생물강해성능.
Objective To test the osteogenetic ability and biocompatibility of a new type of porous morsel blocks of resorbable bioglass bone graft substitute. Methods Thirty healthy New Zealand adult white rabbits were selected to establish bone defect models and randomly assigned into 3 even groups(with 20hind legs in each group)for 3 methods of repairing the defects.The 6 mm×10 to 12 mm defeets were made at the bilateral fenloral condyles of each rabbit.In group A,a porous morsel block of resorbable bioactive glass bone substitute was implanted;in group B,commonly used bioactive glass was implanted;in group C,nothing was implanted.Specimens were collected at 6 and 12 weeks after operation to evaluate the biodegradation and osteogenic properties by gross observation, radiography, Micro-CT and histopathological examinations.ResulIs No local adverse reaction was observed in each group.At 6 weeks.gradual growth of the new bone was observed inside the implants in groups A and B.The amount of new bone in group A was significantly higher than in group B.In group A,the porous morsel material was almost completely degraded,bone defects were completely repaired,and bone trabecula moulding reconstruction was completed.In group B,a great amount of material was not completely degraded in the central area of the graft,while bone defects were not healed in the group C.The percentages of new bone area were 0.30±0.02 in group A,0.17±0.03 in group B and 0.06±0.01 in group C,with significant differences(F=374.202,P<0.001).At 12weeks.the percentages of new bone area were 0.53 ±0.05 in group A,0.39±0.05 in group B and 0.07±0.01 in group C,with significant differences(F=317.243,P<0.001). Conclusion New porous morsel blocks of resorbable bioglass bone graft substitute show superior osteogenetic properties and biodegradability because of their good three dimensional structure.
