CAJ | 학술논문

目的探讨缺氧诱导因子(HIF)-1α和血管内皮生长因子(VEGF)在类风湿关节炎(RA)血管生成中的作用.方法建立大鼠胶原诱导性关节炎(CIA)模型,采用苏木素-伊红(HE)染色评估滑膜病理血管生成、SP法免疫组织化学染色检测HIF-1α和VEGF的表达,动态观察HIF-1α表达与VEGF表达及血管生成之间的关系.结果 CIA大鼠滑膜HIF-1α及VEGF表达增加,且随发病时间的延长而逐渐增加,两者均与滑膜病理血管生成评分呈显著正相关,同时滑膜衬里层和衬里下层HIF-1α表达又与相应部位的VEGF表达呈显著正相关.结论 HIF-1α可能参与上调VEGF表达促进血管生成,从而在RA关节破坏中起一定作用.
목적 탐토결양유도인자(HIF)-1α화혈관내피생장인자(VEGF)재류풍습관절염(RA)혈관생성중적작용.방법 건립대서효원유도성관절염(CIA)모형,채용소목소-이홍(HE)염색평고활막병리혈관생성、SP법면역조직화학염색검측HIF-1α화VEGF적표체,동태관찰HIF-1α표체여VEGF표체급혈관생성지간적관계.결과 CIA대서활막HIF-1α급VEGF표체증가,차수발병시간적연장이축점증가,량자균여활막병리혈관생성평분정현저정상관,동시활막츤리층화츤리하층HIF-1α표체우여상응부위적VEGF표체정현저정상관.결론 HIF-1α가능삼여상조VEGF표체촉진혈관생성,종이재RA관절파배중기일정작용.
Objective To investigate the effect of hypoxia-inducible factor (HIF)-1α and vascular en-dothelial growth factor (VEGF) on the angioge-nesis of rheumatoid arthritis (RA). Methods The collagen-induced arthritis (CIA) model was set up in male Wistar rats. The pathological angiogenesis and expression of HIF-1α and VEGF in the synovia different time points were observed by H&E and immunohistochemistry staining. Results The expressions of HIF-1α and VEGF on CIA synovium were significantly elevated and their expression increased gradually with the prolonged disease course. Both synovial HIF-1α expression and VEGF expression were correlated significantly with the pathological angiogenesis score. Synovial lining and sublining HIF-1α expression were correlated significantly with VEGF expression respectively. Conclusion H1F-1α may play an important role in the pathogenesis of RA by upregulating the expression of VEGF and then promoting angiogenesis.