CAJ | 학술논문

目的探讨冰片对大鼠中枢及周围神经髓鞘的影响.方法 SD大鼠根据灌服冰片石蜡油溶液的剂量不同分为低剂量(10 mL/kg)及高剂量(13mL/kg)2组,每组18只.另设对照组18只,灌服10 mL/kg石蜡油溶液.3组大鼠灌服冰片或石蜡油溶液后24 h、48 h及72 h取血并处死取脑、坐骨神经.进行血清髓鞘碱性蛋白(MAP)检测及透射电镜观察.结果 3个观测时间点中,高、低剂量组大鼠MBP含量明显升高,与对照组比较差异有统计学意义(P<0.05),但高、低剂量组之间差异无统计学意义(P>0.05).灌服冰片后24 h MBP含量最高,随时间延长递减,各时间点之间比较差异有统计学意义(P<0.05).电子显微镜观察大鼠灌服冰片后各时间点均可见中枢神经髓鞘异常,出现外形不规则,板层之间裂开,宽窄不均,纤维缠结现象,以24h改变最明显,随时间延长异常减轻;坐骨神经髓鞘仅在灌服冰片后24 h髓鞘数量出现明显减少,其余未见异常.结论冰片可引起大鼠中枢及周围神经髓鞘出现一定程度的异常.
목적 탐토빙편대대서중추급주위신경수초적영향.방법 SD대서근거관복빙편석사유용액적제량불동분위저제량(10 mL/kg)급고제량(13mL/kg)2조,매조18지.령설대조조18지,관복10 mL/kg석사유용액.3조대서관복빙편혹석사유용액후24 h、48 h급72 h취혈병처사취뇌、좌골신경.진행혈청수초감성단백(MAP)검측급투사전경관찰.결과 3개관측시간점중,고、저제량조대서MBP함량명현승고,여대조조비교차이유통계학의의(P<0.05),단고、저제량조지간차이무통계학의의(P>0.05).관복빙편후24 h MBP함량최고,수시간연장체감,각시간점지간비교차이유통계학의의(P<0.05).전자현미경관찰대서관복빙편후각시간점균가견중추신경수초이상,출현외형불규칙,판층지간렬개,관착불균,섬유전결현상,이24h개변최명현,수시간연장이상감경;좌골신경수초부재관복빙편후24 h수초수량출현명현감소,기여미견이상.결론 빙편가인기대서중추급주위신경수초출현일정정도적이상.
Objective To study the effect of borneol on myelin of the central and peripheral nervous systems. Methods The rats were divided randomly into low-dose borneol treatment group (10 mL/kg,n=18) and high-dose borneol treatment group (13 mL/kg, n=18). Controls (n=18) were chosen and treated with 10 mL/kg paroline. Rats were sacrificed 24, 48 and 72 h after the treatment, and the blood, brain and sciatic nerves of these rats were prepared, respectively; the level of serum myelin basic protein (MBP) was detected and the morphological structure of myelin was observed with transmission electron microscope. Results The content of serum MBP in the 2 treatment groups was advanced markedly as compared with that in the control group 24, 48 and 72 h after the treatment (P<0.05);however, no significant difference was noted between the 2 treatment groups (P>0.05). The content of serum MBP was the highest 24 h after the treatment, gradually decreased following the prolongation of time; significant difference was noted between each 2 time points (P<0.05). Transmission electron microscope indicated abnormality in myelin of the central nerves at all time points, showing inordinate shape, rupture between layers and tangle among fibers; the abnormality was the most obvious 24 h after the treatment, gradually abatement following the prolongation of time; decreased myelin in the sciatic nerves was showed only at 24 h after the treatment, while normal at other time points. Conclusion A certain degree of abnormality of myelin of the central and peripheral nervous systems in rats can be caused by borneol.