CAJ | 학술논문

目的研究应用灯盏花素对供肝进行预处理,减轻大鼠肝移植术后早期缺血再灌注损伤的作用.方法以SD大鼠作为肝移植供、受者,采用随机数字表法将受鼠分为4组.A组和C组供肝未经灯盏花素预处理,B组和D组供肝经含20 mg/L灯盏花素的UW液预处理；A组和B组供肝冷缺血时间为30～40 min,C组和D组为12 h.术后检测各组受鼠的凝血功能、肝功能、血清血栓调节蛋白(TM)含量、凋亡蛋白酶-3 (Caspase-3)活性及核因子-kB(NF-kB)相对表达量,观察各组移植肝组织病理学变化和肝细胞凋亡情况.结果术后3d,C组与D组受鼠死亡率分别为40.0％(8/20)和29.4％(5/17),差异无统计学意义(P＞0.05)；术后4组间凝血功能无明显变化(P＞0.05).与C组比较,术后早期D组肝功能、肝组织病理学改变及肝细胞凋亡均明显改善(P＜0.01),血清TM含量、Caspase-3活性及NF-kB表达量均明显降低(P＜0.01).术后A组和B组的缺血再灌注损伤明显较轻,两组间上述指标的差异均无统计学意义(P＞0.05).结论应用灯盏花素对供肝进行预处理,能够减轻大鼠肝移植术后由冷保存时间较长引起的缺血再灌注损伤,其机制可能与抑制凋亡相关的信号通路和减轻肝脏微循环内皮细胞损伤有关.
목적 연구응용등잔화소대공간진행예처리,감경대서간이식술후조기결혈재관주손상적작용.방법 이SD대서작위간이식공、수자,채용수궤수자표법장수서분위4조.A조화C조공간미경등잔화소예처리,B조화D조공간경함20 mg/L등잔화소적UW액예처리；A조화B조공간랭결혈시간위30～40 min,C조화D조위12 h.술후검측각조수서적응혈공능、간공능、혈청혈전조절단백(TM)함량、조망단백매-3 (Caspase-3)활성급핵인자-kB(NF-kB)상대표체량,관찰각조이식간조직병이학변화화간세포조망정황.결과 술후3d,C조여D조수서사망솔분별위40.0％(8/20)화29.4％(5/17),차이무통계학의의(P＞0.05)；술후4조간응혈공능무명현변화(P＞0.05).여C조비교,술후조기D조간공능、간조직병이학개변급간세포조망균명현개선(P＜0.01),혈청TM함량、Caspase-3활성급NF-kB표체량균명현강저(P＜0.01).술후A조화B조적결혈재관주손상명현교경,량조간상술지표적차이균무통계학의의(P＞0.05).결론 응용등잔화소대공간진행예처리,능구감경대서간이식술후유랭보존시간교장인기적결혈재관주손상,기궤제가능여억제조망상관적신호통로화감경간장미순배내피세포손상유관.
Objective To observe the effect of donor liver pretreated by breviscapine on liver transplantation ischemia/reperfusion injury in rats. Methods SD rats served as liver donors and recipients (n =48 each).The recipients were divided into four groups by random number table.The donors in groups A and C were not pretreated with breviscapine,but those in groups B and D were pretreated with 20 mg/L Breviscapine.The cold ischemia time in donor livers of groups A and B was 30-40 min,and that in groups C and D was 12 h. Clotting function, liver function, serum thrombomodulin,caspase3,and relative activity of NF-kB after liver transplantation were assessed,and the pathological changes and TUNEL apoptosis staining were observed.Results The mortality in groups C and D was 40.0％ (8/20) and 29.4％ (5/17),respectively (P＞0.05).There were no significant changes in coagulation function in all groups after operation. The liver function was improved,pathological lesions were alleviated,and apoptosis rate,serum TM,caspase3 expression and activity of NF-kB in the liver tissues of group D were significantly decreased as compared with group C at 3rd day after operation (P＜0.01),but all these parameters in group B had no significant change compared to group A.Conclusion Pretreatment of donor livers with breviscapine can reduce the ischemia/reperfusion injury and apoptosis after liver transplantation in rats probably by inhibiting the apoptosis-related pathway and alleviating the damage to the endothelial cells of the liver microcirculation.