中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2010年
4期
360-364
,共5页
郑子峥%唐明%苗季%赵敏%黄慧%李婧娴%俞海%李少伟%张军%夏宁邵
鄭子崢%唐明%苗季%趙敏%黃慧%李婧嫻%俞海%李少偉%張軍%夏寧邵
정자쟁%당명%묘계%조민%황혜%리청한%유해%리소위%장군%하저소
中和性表位%免疫非优势表位%戊型肝炎病毒%衣壳
中和性錶位%免疫非優勢錶位%戊型肝炎病毒%衣殼
중화성표위%면역비우세표위%무형간염병독%의각
Neutralizing epitope%Non-immunodominant epitope%Hepatitis E virus%Capoid
目的 探讨戊型肝炎病毒(HEV)衣壳蛋白是否存在除主要免疫优势中和表位aa459～606以外的其他中和表位.方法 通过对几株单克隆抗体及其表位的性质进行分析,对比位于主要免疫优势表位区aM59～606和位于该表位N端序列aa394～458区域的数个表位的中和活性.结果 发现位于aa423～437的表位对应的单抗具有潜在中和活性,不同于已知的HEV中和性表位(aa459～606),该表位是一个线性非免疫优势表位.结论 HEV ORF2 aa423～437为新的潜在的线性非免疫优势中和表位,该发现丰富了对HEV衣壳结构的认识,为HEV预防与治疗提供了新的针对靶点.
目的 探討戊型肝炎病毒(HEV)衣殼蛋白是否存在除主要免疫優勢中和錶位aa459～606以外的其他中和錶位.方法 通過對幾株單剋隆抗體及其錶位的性質進行分析,對比位于主要免疫優勢錶位區aM59～606和位于該錶位N耑序列aa394～458區域的數箇錶位的中和活性.結果 髮現位于aa423～437的錶位對應的單抗具有潛在中和活性,不同于已知的HEV中和性錶位(aa459～606),該錶位是一箇線性非免疫優勢錶位.結論 HEV ORF2 aa423～437為新的潛在的線性非免疫優勢中和錶位,該髮現豐富瞭對HEV衣殼結構的認識,為HEV預防與治療提供瞭新的針對靶點.
목적 탐토무형간염병독(HEV)의각단백시부존재제주요면역우세중화표위aa459～606이외적기타중화표위.방법 통과대궤주단극륭항체급기표위적성질진행분석,대비위우주요면역우세표위구aM59～606화위우해표위N단서렬aa394～458구역적수개표위적중화활성.결과 발현위우aa423～437적표위대응적단항구유잠재중화활성,불동우이지적HEV중화성표위(aa459～606),해표위시일개선성비면역우세표위.결론 HEV ORF2 aa423～437위신적잠재적선성비면역우세중화표위,해발현봉부료대HEV의각결구적인식,위HEV예방여치료제공료신적침대파점.
Objective To search for the neutralizing epitopos on hepatitis E virus (HEV) capsid besides the known neutralizing epitope (aa459-606). Methods By analysis of several strains of monoclonal antibodies against HEV capsid and their recognized epitopes, the neutralizing activity of epitope (aa394-458) at N-terminus was compared with that of an immunodominant neutralizing epitope (an459-606). Re-suits The research showed a novel potential neutralizing epitope in aa423-437 of HEV ORF2 though detec-ting and comparing the characteristics of several antibodies and corresponding determinations. The epitope is a linear non-immunodominant epitope which is different from the other neutralizing epitope in aa459-606.And the amino acids sequence of this novel epitope is conservative. Conclusion ORF2 aa423-437 is a no-vel potential neutralizing leaner epitope of HEV. It is believed that the present work adds fundamental knowl-edge to our understanding of HEV capsid domain and contributes to the prevention and control of this dis-ease.
