中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2012年
5期
320-323
,共4页
陈云祥%李强%王成德%苏志鹏%李伟强%陈贤斌%吴哲褒
陳雲祥%李彊%王成德%囌誌鵬%李偉彊%陳賢斌%吳哲褒
진운상%리강%왕성덕%소지붕%리위강%진현빈%오철포
崔乳素瘤%垂体肿瘤
崔乳素瘤%垂體腫瘤
최유소류%수체종류
Prolactinoma%Pituitary neoplasms
目的 探讨垂体泌乳素(PRL)腺瘤组织中的microRNA(miRNA)表达与腺瘤形成、生长及激素分泌的关系.方法 利用Slexa测序技术分析miRNA在PRL腺瘤和正常垂体前叶组织中的差异表达,并用茎环引物(stem-loop) RT-PCR技术在27例PRL腺瘤和5例正常垂体前叶中加以验证.结果 通过Solexa测序对比PRL腺瘤与正常垂体前叶组织,发现39个丰度较高的miRNA存在差异性表达.进一步通过stem-loop RT-PCR验证,发现4个miRNA下调(miR-130a,miR-199b-3p,miR-200b,miR-125b,P＜0.05);6个miRNA上调(miR-342-3p,miR-432,miR-23b,miR-493,miR-493*,miR-664*,P＜0.05).其中miR-493*,miR-432表达与泌乳素水平呈显著正相关(r=0.47,P＜0.05；r=0.528,P＜0.01)；miR-342-3p表达与肿瘤侵袭性呈正相关(r=0.402,P＜0.05).结论 正常垂体前叶/PRL腺瘤、侵袭型/局限型PRL腺瘤、不同激素分泌水平PRL腺瘤之间存在着miRNA差异表达,提示miRNA可能参与垂体泌乳素腺瘤的成瘤、生长及激素分泌等生理过程.
目的 探討垂體泌乳素(PRL)腺瘤組織中的microRNA(miRNA)錶達與腺瘤形成、生長及激素分泌的關繫.方法 利用Slexa測序技術分析miRNA在PRL腺瘤和正常垂體前葉組織中的差異錶達,併用莖環引物(stem-loop) RT-PCR技術在27例PRL腺瘤和5例正常垂體前葉中加以驗證.結果 通過Solexa測序對比PRL腺瘤與正常垂體前葉組織,髮現39箇豐度較高的miRNA存在差異性錶達.進一步通過stem-loop RT-PCR驗證,髮現4箇miRNA下調(miR-130a,miR-199b-3p,miR-200b,miR-125b,P＜0.05);6箇miRNA上調(miR-342-3p,miR-432,miR-23b,miR-493,miR-493*,miR-664*,P＜0.05).其中miR-493*,miR-432錶達與泌乳素水平呈顯著正相關(r=0.47,P＜0.05；r=0.528,P＜0.01)；miR-342-3p錶達與腫瘤侵襲性呈正相關(r=0.402,P＜0.05).結論 正常垂體前葉/PRL腺瘤、侵襲型/跼限型PRL腺瘤、不同激素分泌水平PRL腺瘤之間存在著miRNA差異錶達,提示miRNA可能參與垂體泌乳素腺瘤的成瘤、生長及激素分泌等生理過程.
목적 탐토수체비유소(PRL)선류조직중적microRNA(miRNA)표체여선류형성、생장급격소분비적관계.방법 이용Slexa측서기술분석miRNA재PRL선류화정상수체전협조직중적차이표체,병용경배인물(stem-loop) RT-PCR기술재27례PRL선류화5례정상수체전협중가이험증.결과 통과Solexa측서대비PRL선류여정상수체전협조직,발현39개봉도교고적miRNA존재차이성표체.진일보통과stem-loop RT-PCR험증,발현4개miRNA하조(miR-130a,miR-199b-3p,miR-200b,miR-125b,P＜0.05);6개miRNA상조(miR-342-3p,miR-432,miR-23b,miR-493,miR-493*,miR-664*,P＜0.05).기중miR-493*,miR-432표체여비유소수평정현저정상관(r=0.47,P＜0.05；r=0.528,P＜0.01)；miR-342-3p표체여종류침습성정정상관(r=0.402,P＜0.05).결론 정상수체전협/PRL선류、침습형/국한형PRL선류、불동격소분비수평PRL선류지간존재착miRNA차이표체,제시miRNA가능삼여수체비유소선류적성류、생장급격소분비등생리과정.
Objective To explore the relationship between the prolactinoma-related microRNAs (miRNA) and the development,growth and hormone secretion of prolactinoma.Methods The technique of Solexa sequencing was employed to analyze the differential expressions of prolactinoma and normal anterior pituitary gland samples. And the stem-loop real-time polymerase chain reaction (PCR) was utilized for confirmation.Results According to the differentially expressed profiles of miRNAs,4 miRNAs were downregulated (miR-130a,miR-199b-3p,miR-200b,miR-125b,P＜0.05) and6 miRNAs up-regulated (miR-342-3p,miR-432,miR-23b,miR-493,miR-493 *,miR-664 *,P ＜ 0.05).The expression levels of miR-493 * and miR-432 had a significant positive correlation with the serum level of prolactin ( r =0.47,P ＜0.05 ; r =0.528,P ＜ 0.01 ) while miR-342-3p a significantly positive correlation with the invasiveness ( r =0.402,P ＜ 0.05 ).Conclusion miRNAs are differentially expressed between normal anterior pituitary gland and prolactinomas,between invasive and localized prolactinomas and among different hormone secretion levels. It suggests that miRNAs may be involved in the physiological process of development,growth and hormone secretion of prolactinoma.
