CAJ | 학술논문

目的观察细胞因子信号转导抑制分子(SOCS)在原发性胆汁性肝硬化(PBC)患者树突状细胞(DC)及其表型和分泌细胞因子的变化,以研究SOCS在PBC发病机制中的作用.方法对10例PBC患者和8名健康人,用流式细胞术(FCM)分析其DC表型CD83、CD86和人类白细胞抗原DR(HLA-DR),用酶联免疫吸附测定法(ELISA)检测DC培养上清液中细胞因子白细胞介素-10(IL10)、IL-12和干扰素-γ(IFN－γ)含量,用免疫印迹法(WB)测定DC中SOCS1和SOCS3水平；并评价分析这些指标在2组中的变化特征.结果 PBC患者外周血中DC细胞表型CD83、CD86和HLA-DR 的表达率分别为(79.4±4.8)％、(86.5±6.3)％和(90.0±3.5)％,均高于健康对照组的表达率[(68.3±4.1)％、(74.2±6.3)％和(83.6±7.6)％,t值分别为5.340、4.120和2.514,P均＜0.05]；DC分泌的IL-12和IFN-γ含量分别为(53.5±11.1)、(32.0±9.0)ng/L,与健康对照组的细胞因子含量[(32.1±10.7)、(15.4±8.1)ng/L]相比,IL-12和IFN-γ均显著升高(t值分别为4.123和3.818,P均＜0.01)；IL-10含量为(7.0±4.6) ng/L,与健康对照组[(5.8±4.2) ng/L]相比,差异无统计学意义(t=0.563,P＞0.05)；WB检测PBC组DC中SOCS1和SOCS3的表达比健康对照组明显降低.结论 PBC患者体内DC更倾向于成熟状态,其抗原递呈能力明显增强,SOCS的低表达可能与免疫平衡紊乱和免疫耐受破坏有关.
목적 관찰세포인자신호전도억제분자(SOCS)재원발성담즙성간경화(PBC)환자수돌상세포(DC)급기표형화분비세포인자적변화,이연구SOCS재PBC발병궤제중적작용.방법 대10례PBC환자화8명건강인,용류식세포술(FCM)분석기DC표형CD83、CD86화인류백세포항원DR(HLA-DR),용매련면역흡부측정법(ELISA)검측DC배양상청액중세포인자백세포개소-10(IL10)、IL-12화간우소-γ(IFN－γ)함량,용면역인적법(WB)측정DC중SOCS1화SOCS3수평；병평개분석저사지표재2조중적변화특정.결과 PBC환자외주혈중DC세포표형CD83、CD86화HLA-DR 적표체솔분별위(79.4±4.8)％、(86.5±6.3)％화(90.0±3.5)％,균고우건강대조조적표체솔[(68.3±4.1)％、(74.2±6.3)％화(83.6±7.6)％,t치분별위5.340、4.120화2.514,P균＜0.05]；DC분비적IL-12화IFN-γ함량분별위(53.5±11.1)、(32.0±9.0)ng/L,여건강대조조적세포인자함량[(32.1±10.7)、(15.4±8.1)ng/L]상비,IL-12화IFN-γ균현저승고(t치분별위4.123화3.818,P균＜0.01)；IL-10함량위(7.0±4.6) ng/L,여건강대조조[(5.8±4.2) ng/L]상비,차이무통계학의의(t=0.563,P＞0.05)；WB검측PBC조DC중SOCS1화SOCS3적표체비건강대조조명현강저.결론 PBC환자체내DC경경향우성숙상태,기항원체정능력명현증강,SOCS적저표체가능여면역평형문란화면역내수파배유관.
Objective To study the role of suppressor of cytokine signaling ( SOCS ) in the pathogenesis of primary biliary cirrhosis( PBC),the levels of SOCS protein and the changes of function of dendritic cell(DC) were respectively observed from PBC patients.Methods The study population consisted of 10 patients of PBC and 8 healthy controls.Phenotypic analysis of cultured peripheral blood mononuclear cells (PBMC)-derived DC was performed by flow cytometry (FCM),such as CD83,CD86 and human leukocyte antigen DR (HLA-DR).The levels of interleukin-10 (IL-10),interferon-γ( IFN-γ) and IL-12 in culture supematant of DC were measured by enzyme linked immunosorbent assay ( ELISA ).The protein levels of SOCS1 and SOCS3 were detected by Western blot ( WB ).The features of changes in these parameters were analyzed between the two groups.Results The expression of CD83,CD86 and HLA-DR in PBC patients were ( 79.4 ± 4.8 ) ％,( 86.5 ± 6.3 ) ％ and (90.0 ± 3.5 ) ％,which were significantly higher than those in healthy control group[ (68.3 ±4.1 )％,(74.2 ±6.3)％ and (83.6 ±7.6)％ ],respectively (t =5.340,4.120,2.514,P ＜0.05).The levels of IL-12 and IFN-γin PBC patients were (53.5 ± 11.1)and (32.0 ±9.0) ng/L,which were significantly higher than those in healthy control group[ (32.1 ± 10.7) and (15.4 ± 8.1 ) ng/L; t =4.123,3.818,P ＜ 0.01 ].There were not any significant difference of IL-10 level between PBC patients [ (7.0 ± 4.6) ng/L ] and the healthy controls [ ( 5.8 ± 4.2) ng/L; t =0.563,P ＞ 0.05 ].The proteins levels of SOCS1 and SOCS3 in PBMC-derived DC from PBC group were decreased significantly than those in healthy control group.Conclusions The results suggest that the PBMC-derived DC in PBC patients has greater ability of potent maturation and antigen presentation function.The decreased expression of SOCS levels may be associated with the excessive immunological reaction and the breakdown of self-tolerance.