背景:颞叶皮质与人类和动物的学习记忆能力有关,它不仅接受听觉的投射纤维,还与许多空间认知相关的脑区有密切关系.目的:观察单侧颞叶皮质脑梗死大鼠经溶栓胶囊治疗后空间认识能力的改善情况.设计:完全随机对照实验.单位:解放军第一七五医院神经内科,解放军总医院神经内科及中国科学院心理研究所. 材料:实验于1998-08/1999-02在中国科学院心理研究所脑功能研究室完成,选择SD大鼠28只,随机分为3组:脑梗死组10只,治疗组10只,假手术组8只.方法:立体定向光化学诱导制作脑梗死大鼠模型.水合氯醛麻醉,尾静脉注射玫瑰红溶液20 mg/kg,左侧颞部切开头皮,暴露颞骨,光导纤维引导卤素灯冷光源,定向照射20 min,面积2mm2.照射20 min后治疗组腹腔注射溶栓胶囊生理盐水溶液(40 g/L)200 mg/kg,脑梗死组腹腔内注射等量生理盐水.假手术组尾静脉及腹腔注射生理盐水外,手术照射条件相同.主要观察指标:各组大鼠在Morris水迷宫中的训练成绩,包括:①反应时.②搜索策略(分为边缘式记1分,随机式记2分,趋向式记3分,直线式记4分).结果:28只大鼠均进入结果分析.①各组大鼠平均反应时比较:治疗组和假手术组短于脑梗死组[第1天:58,50,65 s;第2天:24,27,46 s;第3天:14,17,20 s;第4天:11,9,15 s,(P＜0.01)],治疗组与假手术组基本接近(P＞0.05).②搜索策略评分:第1天可见假手术组及治疗组稍高于脑梗死组,但不稳定(F=1.167,P＞0.05),第2天假手术组高于脑梗死组[2.9,2.3分,(F=5.5,P＜0.05)],但治疗组与脑梗死组基本接近[2.6,2.3分,(F=3.34,P＞0.05)],第3,4天治疗组均高于脑梗死组[3.5,2.7分;3.7,3.3分,(F=8.92,14,P＜0.01)].治疗组与假手术组基本接近(P＞0.05).结论:脑梗死早期给予溶栓胶囊治疗后,反应时明显缩短,搜索策略逐渐由边缘式和随机式过渡到趋向式和直线式,说明溶栓胶囊可改善脑缺血后的认知功能障碍,提高脑梗死大鼠的训练成绩.
揹景:顳葉皮質與人類和動物的學習記憶能力有關,它不僅接受聽覺的投射纖維,還與許多空間認知相關的腦區有密切關繫.目的:觀察單側顳葉皮質腦梗死大鼠經溶栓膠囊治療後空間認識能力的改善情況.設計:完全隨機對照實驗.單位:解放軍第一七五醫院神經內科,解放軍總醫院神經內科及中國科學院心理研究所. 材料:實驗于1998-08/1999-02在中國科學院心理研究所腦功能研究室完成,選擇SD大鼠28隻,隨機分為3組:腦梗死組10隻,治療組10隻,假手術組8隻.方法:立體定嚮光化學誘導製作腦梗死大鼠模型.水閤氯醛痳醉,尾靜脈註射玫瑰紅溶液20 mg/kg,左側顳部切開頭皮,暴露顳骨,光導纖維引導滷素燈冷光源,定嚮照射20 min,麵積2mm2.照射20 min後治療組腹腔註射溶栓膠囊生理鹽水溶液(40 g/L)200 mg/kg,腦梗死組腹腔內註射等量生理鹽水.假手術組尾靜脈及腹腔註射生理鹽水外,手術照射條件相同.主要觀察指標:各組大鼠在Morris水迷宮中的訓練成績,包括:①反應時.②搜索策略(分為邊緣式記1分,隨機式記2分,趨嚮式記3分,直線式記4分).結果:28隻大鼠均進入結果分析.①各組大鼠平均反應時比較:治療組和假手術組短于腦梗死組[第1天:58,50,65 s;第2天:24,27,46 s;第3天:14,17,20 s;第4天:11,9,15 s,(P＜0.01)],治療組與假手術組基本接近(P＞0.05).②搜索策略評分:第1天可見假手術組及治療組稍高于腦梗死組,但不穩定(F=1.167,P＞0.05),第2天假手術組高于腦梗死組[2.9,2.3分,(F=5.5,P＜0.05)],但治療組與腦梗死組基本接近[2.6,2.3分,(F=3.34,P＞0.05)],第3,4天治療組均高于腦梗死組[3.5,2.7分;3.7,3.3分,(F=8.92,14,P＜0.01)].治療組與假手術組基本接近(P＞0.05).結論:腦梗死早期給予溶栓膠囊治療後,反應時明顯縮短,搜索策略逐漸由邊緣式和隨機式過渡到趨嚮式和直線式,說明溶栓膠囊可改善腦缺血後的認知功能障礙,提高腦梗死大鼠的訓練成績.
배경:섭협피질여인류화동물적학습기억능력유관,타불부접수은각적투사섬유,환여허다공간인지상관적뇌구유밀절관계.목적:관찰단측섭협피질뇌경사대서경용전효낭치료후공간인식능력적개선정황.설계:완전수궤대조실험.단위:해방군제일칠오의원신경내과,해방군총의원신경내과급중국과학원심리연구소. 재료:실험우1998-08/1999-02재중국과학원심리연구소뇌공능연구실완성,선택SD대서28지,수궤분위3조:뇌경사조10지,치료조10지,가수술조8지.방법:입체정향광화학유도제작뇌경사대서모형.수합록철마취,미정맥주사매괴홍용액20 mg/kg,좌측섭부절개두피,폭로섭골,광도섬유인도서소등랭광원,정향조사20 min,면적2mm2.조사20 min후치료조복강주사용전효낭생리염수용액(40 g/L)200 mg/kg,뇌경사조복강내주사등량생리염수.가수술조미정맥급복강주사생리염수외,수술조사조건상동.주요관찰지표:각조대서재Morris수미궁중적훈련성적,포괄:①반응시.②수색책략(분위변연식기1분,수궤식기2분,추향식기3분,직선식기4분).결과:28지대서균진입결과분석.①각조대서평균반응시비교:치료조화가수술조단우뇌경사조[제1천:58,50,65 s;제2천:24,27,46 s;제3천:14,17,20 s;제4천:11,9,15 s,(P＜0.01)],치료조여가수술조기본접근(P＞0.05).②수색책략평분:제1천가견가수술조급치료조초고우뇌경사조,단불은정(F=1.167,P＞0.05),제2천가수술조고우뇌경사조[2.9,2.3분,(F=5.5,P＜0.05)],단치료조여뇌경사조기본접근[2.6,2.3분,(F=3.34,P＞0.05)],제3,4천치료조균고우뇌경사조[3.5,2.7분;3.7,3.3분,(F=8.92,14,P＜0.01)].치료조여가수술조기본접근(P＞0.05).결론:뇌경사조기급여용전효낭치료후,반응시명현축단,수색책략축점유변연식화수궤식과도도추향식화직선식,설명용전효낭가개선뇌결혈후적인지공능장애,제고뇌경사대서적훈련성적.
BACKGROUND: Temporal cortex is related to human and animal's learning and memory capability, it not only accepts the casting fibers of auditory sense, but also closely connects with many brain areas where are related to spatial cognition.OBJECTIVE: To observe the improving effect of thrombolytic capsule on spatial cognitive capability of rats with lateral temporal cortical infarction DESIGN: Completely randomized controlled study SETTING: Department of Neurology, the 175th Hospital of Chinese PLA;Department of Neurology, General Hospital of Chinese PLA; Institute of Psychology of Chinese Academy of Science MATERIALS: This experiment was carried out at Brain Function Laboratory of Institute of Psychology of Chinese Academy of Science from August 1998 to February 1999, 28 SD rats were randomly divided into 3 groups,namely brain stroke group with 10 rats, treatment group with 10 rats and sham operation group with 8 rats.METHODS: Brain infarction model was established on SD rats by using three-dimensional directional photochemistry induction. Rats were anaesthetized with hydrochloride and intravenously injected of rose pink solution in dosage of 20 mg/kg from tail vein. Then incision was made on the left temporal scalp to expose temporal bone, cold photosource with the guidance of optical fiber was used for directional illumination for 20 minutes in area of 2 mm2. Twenty minutes later, rats in treatment group were given intraperitoneal injection of thrombolytic capsule physiological saline of 40 g/L in dosage of 200 mg/kg, which replaced by the same volume of physiological saline in brain stroke group. Rats in sham-operation group received the same operative lamination and injected with the same volume of physiological saline from tail vein and intraperitoneal cavity.MAIN OUTCOME MEASURES: Achievement of Morris water labyrinth(edge type scored 1, randomize type of 2, trend type of 3 and straight-line type of 4).age response time: it was shortened in treatment group and sham operation group than brain infarction group [ on 1st day: 58,50,65 s; 2nd day: 24,27,46 s; 3rd day: 14,17,20 s; 4th day: 11,9,15 s, (P ＜ 0.01 )], but it was similar ing strategy grades: on the 1st day, scores were slightly higher in treatment group and sham operation group than infarction group, but were instable (F=1.167, P ＞ 0.05). On the 2nd day, it was higher in sham operation group than brain stroke group[2.9,2.3, (F=5.5 ,P ＜ 0.05)], but it was similar between treatment group and infarction group[2.6,2.3 scores, (F=3.34,P ＞ 0.05)]. on the 3rd and 4th day, it was higher in treatment group than infarction group [ 3.5,2.7 minutes; 3.7,3.3 minutes, (F=8.92, 14, P ＞ 0.05)]and similar between treatment group and sham operation group(P ＞ 0.05).CONCLUSION: Rat response time was reduced by thrombolytic capsule during the early time of cerebral infarction, changing searching tactics gradually from randomized and edge type to tendency type and straight line type, suggesting thrombolytic capsule can improve the post-ischemia cognitive function, raise training record of cerebral infarctional rats.
