中国兽医学报
中國獸醫學報
중국수의학보
CHINESE JOURNAL OF VETERINARY SCIENCE
2009年
7期
845-848
,共4页
闫芳%岳文斌%刘娟%李绪英%赵宇军%吉文汇%刘风波%吴倩%任家琰%化丽珍
閆芳%嶽文斌%劉娟%李緒英%趙宇軍%吉文彙%劉風波%吳倩%任傢琰%化麗珍
염방%악문빈%류연%리서영%조우군%길문회%류풍파%오천%임가염%화려진
鸡传染性支气管炎病毒%分离%鉴定
鷄傳染性支氣管炎病毒%分離%鑒定
계전염성지기관염병독%분리%감정
infectious bronchitis virus%isolation%characterizing
从山西各地区疑似鸡传染性支气管炎(IB)的病料中,分离到5株鸡传染性支气管炎病毒(IBV)分离株,并对分离病毒进行了病毒形态观察、对鸡新城疫病毒(NDV)的干扰、鸡胚致病性试验、动物回归试验、血凝特性试验、病毒理化特性测定等生物特性鉴定及IBV N基因特异性片段的检测.电镜观察,可见直径为60～120 am,有囊膜及纤突呈冠状排列的病毒粒子;对NDV有明显的干扰作用;分离株的传代物均有明显的致鸡胚矮小化作用;动物回归感染死亡鸡肾脏病变明显,表现肾脏肿大、花斑肾现象,输尿管内充塞大量尿酸盐;无直接血凝性,经1%胰酶处理后可凝集鸡红细胞;分离株对乙醚和氯仿敏感;采用反转录-聚合酶链式反应(RT-PCR)对分离毒株进行扩增,结果均扩增出特异N基因核酸片段.
從山西各地區疑似鷄傳染性支氣管炎(IB)的病料中,分離到5株鷄傳染性支氣管炎病毒(IBV)分離株,併對分離病毒進行瞭病毒形態觀察、對鷄新城疫病毒(NDV)的榦擾、鷄胚緻病性試驗、動物迴歸試驗、血凝特性試驗、病毒理化特性測定等生物特性鑒定及IBV N基因特異性片段的檢測.電鏡觀察,可見直徑為60～120 am,有囊膜及纖突呈冠狀排列的病毒粒子;對NDV有明顯的榦擾作用;分離株的傳代物均有明顯的緻鷄胚矮小化作用;動物迴歸感染死亡鷄腎髒病變明顯,錶現腎髒腫大、花斑腎現象,輸尿管內充塞大量尿痠鹽;無直接血凝性,經1%胰酶處理後可凝集鷄紅細胞;分離株對乙醚和氯倣敏感;採用反轉錄-聚閤酶鏈式反應(RT-PCR)對分離毒株進行擴增,結果均擴增齣特異N基因覈痠片段.
종산서각지구의사계전염성지기관염(IB)적병료중,분리도5주계전염성지기관염병독(IBV)분리주,병대분리병독진행료병독형태관찰、대계신성역병독(NDV)적간우、계배치병성시험、동물회귀시험、혈응특성시험、병독이화특성측정등생물특성감정급IBV N기인특이성편단적검측.전경관찰,가견직경위60～120 am,유낭막급섬돌정관상배렬적병독입자;대NDV유명현적간우작용;분리주적전대물균유명현적치계배왜소화작용;동물회귀감염사망계신장병변명현,표현신장종대、화반신현상,수뇨관내충새대량뇨산염;무직접혈응성,경1%이매처리후가응집계홍세포;분리주대을미화록방민감;채용반전록-취합매련식반응(RT-PCR)대분리독주진행확증,결과균확증출특이N기인핵산편단.
Five field strains of infectious bronchitis virus (IBV) were isolated from suspected flocks from different time and different regions of Shanxi province,respectively,and characterized by a series of systematic identification assays,such as morphological observation by electron-microscope,interfering with the propagation of NDV,virus pathological role to chicken embryo,virus pathological role to SPF chickens,hemagglutination activity,physiscochemical,and reverse transcription polymerase chain reaction(RT-PCR).The results showed:The typical coronavirus which the spherical virions 60-120 nm in diameter and surface covered with spike like corona were observed under electron-microscope)The propagation of NDV strain was seriously interfered by the 5 isolates respectively;The embryonated chicken egg passages of the 5 isolates could dwarf with chicken embryos;The five isolates had no hemagglutination activity,but after treatment with 1% trypsin,it can agglutinate chicken red blood cell.The strains are sensitive to chloroform and ethyl ether.The SPF chickens which inoculated with the 5 isolates showed clinical sign and result in respiratory and kidney diseases,flower-steak,and swollen with severe urate deposition.The specific fragments of N gene of the 5 isolates were amplified by RT-PCR,respectiveiy.On the basis of all above mentioned results,the 5 isolates were classified as IBV.The study got a good preparedness for further study on molecular epidemioogy of the 5 IBV isolates.
