中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2009年
7期
1170-1173
,共4页
陆焱%邵耀明%陈文%朱华淳%徐明%陈国千
陸焱%邵耀明%陳文%硃華淳%徐明%陳國韆
륙염%소요명%진문%주화순%서명%진국천
脓毒症%小檗碱%高迁移率族蛋白质类
膿毒癥%小檗堿%高遷移率族蛋白質類
농독증%소벽감%고천이솔족단백질류
Sepsis%Berberine%High mobility group proteins
目的 观察小檗碱对脓毒症小鼠血清高迁移率族蛋白B1(HMGB1)水平和存活率的影响.方法 采用盲肠结扎穿孔术建立小鼠脓毒症模型.脓毒症小鼠随机分为对照组和小檗碱处理组,后者以5 mg/kg剂量于盲肠结扎穿孔术前1 h和术后24、48、72、96 h分别于腹腔内注射小檗碱,观察小檗碱处理后脓毒症小鼠不同时间血清HMGB1水平和存活率的变化.结果 脓毒症小鼠经小檗碱处理后血清HMGB1水平显著降低(P＜0.05),术后120 h存活率由32%提高至68%(P＜0.01).结论 小檗碱能降低脓毒症小鼠血清HMGB1水平并提高存活率.
目的 觀察小檗堿對膿毒癥小鼠血清高遷移率族蛋白B1(HMGB1)水平和存活率的影響.方法 採用盲腸結扎穿孔術建立小鼠膿毒癥模型.膿毒癥小鼠隨機分為對照組和小檗堿處理組,後者以5 mg/kg劑量于盲腸結扎穿孔術前1 h和術後24、48、72、96 h分彆于腹腔內註射小檗堿,觀察小檗堿處理後膿毒癥小鼠不同時間血清HMGB1水平和存活率的變化.結果 膿毒癥小鼠經小檗堿處理後血清HMGB1水平顯著降低(P＜0.05),術後120 h存活率由32%提高至68%(P＜0.01).結論 小檗堿能降低膿毒癥小鼠血清HMGB1水平併提高存活率.
목적 관찰소벽감대농독증소서혈청고천이솔족단백B1(HMGB1)수평화존활솔적영향.방법 채용맹장결찰천공술건립소서농독증모형.농독증소서수궤분위대조조화소벽감처리조,후자이5 mg/kg제량우맹장결찰천공술전1 h화술후24、48、72、96 h분별우복강내주사소벽감,관찰소벽감처리후농독증소서불동시간혈청HMGB1수평화존활솔적변화.결과 농독증소서경소벽감처리후혈청HMGB1수평현저강저(P＜0.05),술후120 h존활솔유32%제고지68%(P＜0.01).결론 소벽감능강저농독증소서혈청HMGB1수평병제고존활솔.
Objective To investigate the effect of berberine on serum high mobility group box 1 (HMGB1) level and survival rate in sepsis mice.Methods Mouse sepsis model was established using cecal ligation and puncture (CLP).Spesis mice were randomly divided into control group and berberine-treated group which were respectively administered (i.p.) 1 h before CLP and 24,48,72 and 96 h after CLP with 5 mg/kg berberine.The changes of serum HMGB1 level and survival rate were observed at several time points.Results Serum HMGB1 level were markedly decreased (P＜0.05),and the survival rate increased from 32% to 68% at 120 h after CLP (P＜0.01),after the mice were treated with berberine.Conclusions Berberine decreases serum HMGB1 level,and confers protection against sepsis lethality.
