动物学研究
動物學研究
동물학연구
ZOOLOGICAL RESEARCH
2009年
6期
633-638
,共6页
马莉%孔清华%赵树华%魏云虎%毛炳宇
馬莉%孔清華%趙樹華%魏雲虎%毛炳宇
마리%공청화%조수화%위운호%모병우
Sox2%3'非翻译区%转录后调控%非洲爪蟾
Sox2%3'非翻譯區%轉錄後調控%非洲爪蟾
Sox2%3'비번역구%전록후조공%비주조섬
Sox2%3'UTR%Post-transcriptional regulation%Xenopus laevis
转录因子Sox2是脊椎动物早期发育中最早表达的神经系统特异性基因之一,同时在干细胞的维持中也起着关键作用.通过生物信息学分析,作者发现在脊椎动物Sox2 mRNA 3'非翻译区中存在4段非常保守的富含AU的区域.将这些片段按照不同的组合克隆到GFP和荧光素酶两种报告基因载体中,在非洲爪蟾胚胎和培养细胞中检测了这些片段对报告基因表达的影响.结果显示,Sox2的3'UTR可影响报告基因的表达水平,特别是其中的保守片段2可显著提高报告基因的表达水平,表明Sox2 3'非翻译区有可能参与Sox2表达的转录后调控.
轉錄因子Sox2是脊椎動物早期髮育中最早錶達的神經繫統特異性基因之一,同時在榦細胞的維持中也起著關鍵作用.通過生物信息學分析,作者髮現在脊椎動物Sox2 mRNA 3'非翻譯區中存在4段非常保守的富含AU的區域.將這些片段按照不同的組閤剋隆到GFP和熒光素酶兩種報告基因載體中,在非洲爪蟾胚胎和培養細胞中檢測瞭這些片段對報告基因錶達的影響.結果顯示,Sox2的3'UTR可影響報告基因的錶達水平,特彆是其中的保守片段2可顯著提高報告基因的錶達水平,錶明Sox2 3'非翻譯區有可能參與Sox2錶達的轉錄後調控.
전록인자Sox2시척추동물조기발육중최조표체적신경계통특이성기인지일,동시재간세포적유지중야기착관건작용.통과생물신식학분석,작자발현재척추동물Sox2 mRNA 3'비번역구중존재4단비상보수적부함AU적구역.장저사편단안조불동적조합극륭도GFP화형광소매량충보고기인재체중,재비주조섬배태화배양세포중검측료저사편단대보고기인표체적영향.결과현시,Sox2적3'UTR가영향보고기인적표체수평,특별시기중적보수편단2가현저제고보고기인적표체수평,표명Sox2 3'비번역구유가능삼여Sox2표체적전록후조공.
The transcription factor Sox2 is one of the earliest determinants of the neural system in vertebrate and plays crucial roles in stem cell maintenance. Through bioinformatical analysis, we found that the 3'untranslated regions (3'UTR) of vertebrate Sox2 mRNAs (especially the 300 bases at the most 3' end) are highly conserved and contain four conserved AU rich fragments. Through reporter gene analysis, we evaluated the effects of the Sox2 full length 3'UTR and the conserved fragments on gene expression in Xenopus laevis embryos and cultured cells. The results showed that the conserved fragment 2 from the 3'UTR of Xenoups laevis Sox2 was able to increase the reporter gene expression significantly, indicating the possibility that the expression level of Sox2 might be regulated post-transcriptionally through its 3'UTR.