CAJ | 학술논문

建立由高效液相色谱法检测饼干中三聚氰胺的方法.样品用1%三氯乙酸和2.2%乙酸铅超声提取,色谱柱为Hypersil ODS 100 mm×4.6 mm,5 μm,流动相为乙腈∶辛烷磺酸钠离子对试剂(pH 3)=15∶85(V/V),流速为1.0 mL· min～(-1),检测波长为210 nm,柱温为40 ℃.三聚氰胺在0.2～10 μg·mL～(-1) 范围内,有良好的线性关系.在空白样品中添加1、2和4 μg·mL～(-1) 的标准工作液,平均回收率在80.5%～93.5%之间,相对标准偏差为7.7% (n=9).
건립유고효액상색보법검측병간중삼취청알적방법.양품용1%삼록을산화2.2%을산연초성제취,색보주위Hypersil ODS 100 mm×4.6 mm,5 μm,류동상위을정∶신완광산납리자대시제(pH 3)=15∶85(V/V),류속위1.0 mL· min～(-1),검측파장위210 nm,주온위40 ℃.삼취청알재0.2～10 μg·mL～(-1) 범위내,유량호적선성관계.재공백양품중첨가1、2화4 μg·mL～(-1) 적표준공작액,평균회수솔재80.5%～93.5%지간,상대표준편차위7.7% (n=9).
A high performance liquid chromatography method was developed for the determination of melamine in biscuits. The biscuit samples were extracted by 1% trichloroacetic acid solution and 2.2% lead acetate,and melamine was separated on a Hypersil ODS C_18 column (100 mm×4.6 mm,5 μm ). Acetonitrile and sodium 1-octanesulfonate solution(pH 3)(15∶85,V/V) were used as mobile phase at a flow rate of 1.0 mL·min～(-1). The detective wavelength was set as 210 nm by an ultraviolet detector.The temperature of column was 40 ℃. The linear ranges for melamine were 0.2-10 μg·mL～(-1). The average recovery rate of this method varied from 80.5% to 93.5% with the relative standard deviation of 7.7% (n=9) when blank sample was fortified standard working solution of 1,2 and 4 μg·mL～(-1).