光谱学与光谱分析
光譜學與光譜分析
광보학여광보분석
SPECTROSCOPY AND SPECTRAL ANALYSIS
2010年
3期
820-824
,共5页
郑春霞%陈波浪%贾宏涛%盛建东%潘新燕
鄭春霞%陳波浪%賈宏濤%盛建東%潘新燕
정춘하%진파랑%가굉도%성건동%반신연
沙冬青%Pb~(2+)%内源激素
沙鼕青%Pb~(2+)%內源激素
사동청%Pb~(2+)%내원격소
Ammopiptanthus%Pb~(2+)%Hormone
利用原子发射光谱和高效液相色谱系统地分析研究了内蒙沙冬青(Ammopiptanthus mongolicus (Maxim.)Chengf.)和新疆沙冬青(A.nanus(M Pop.)Chengf.)(韦思奇,1998)发芽前和发芽后赤霉素(GA_3)、生长素(IAA)、脱落酸(ABA)3种内源激素的变化和不同浓度Pb~(2+)胁迫对沙冬青内源激素的影响.研究发现:(1)发芽后比发芽前新疆沙冬青IAA含量降低率为77.80%,ABA含量降低率为98.90%;蒙古沙冬青IAA含量降低率为75.80%,ABA含量降低率为66.20%;2种沙冬青发芽前后GA_3含量变化不大.(2)随Pb~(2+)浓度的提高(20~1 500 mg·L~(-1)),IAA含量降低十分显著,高浓度(大于1 000 mg·L~(-1))Pb~(2+)胁迫对GA_3含量才有影响,ABA含量没有规律性的变化;在Pb~(2+)胁迫下,蒙占沙冬青中3种内源激素含量均高于新疆沙冬青.(3)沙冬青幼苗中Pb~(2+)分布特征均为根>茎>叶.(4)高浓度Pb~(2+)胁迫对沙冬青叶片细胞产生了不可逆的伤害.研究结果为进一步研究荒漠地区特有的常绿阔叶灌木沙冬青的生长特征和抗逆机制提供了可参考的依据.
利用原子髮射光譜和高效液相色譜繫統地分析研究瞭內矇沙鼕青(Ammopiptanthus mongolicus (Maxim.)Chengf.)和新疆沙鼕青(A.nanus(M Pop.)Chengf.)(韋思奇,1998)髮芽前和髮芽後赤黴素(GA_3)、生長素(IAA)、脫落痠(ABA)3種內源激素的變化和不同濃度Pb~(2+)脅迫對沙鼕青內源激素的影響.研究髮現:(1)髮芽後比髮芽前新疆沙鼕青IAA含量降低率為77.80%,ABA含量降低率為98.90%;矇古沙鼕青IAA含量降低率為75.80%,ABA含量降低率為66.20%;2種沙鼕青髮芽前後GA_3含量變化不大.(2)隨Pb~(2+)濃度的提高(20~1 500 mg·L~(-1)),IAA含量降低十分顯著,高濃度(大于1 000 mg·L~(-1))Pb~(2+)脅迫對GA_3含量纔有影響,ABA含量沒有規律性的變化;在Pb~(2+)脅迫下,矇佔沙鼕青中3種內源激素含量均高于新疆沙鼕青.(3)沙鼕青幼苗中Pb~(2+)分佈特徵均為根>莖>葉.(4)高濃度Pb~(2+)脅迫對沙鼕青葉片細胞產生瞭不可逆的傷害.研究結果為進一步研究荒漠地區特有的常綠闊葉灌木沙鼕青的生長特徵和抗逆機製提供瞭可參攷的依據.
이용원자발사광보화고효액상색보계통지분석연구료내몽사동청(Ammopiptanthus mongolicus (Maxim.)Chengf.)화신강사동청(A.nanus(M Pop.)Chengf.)(위사기,1998)발아전화발아후적매소(GA_3)、생장소(IAA)、탈락산(ABA)3충내원격소적변화화불동농도Pb~(2+)협박대사동청내원격소적영향.연구발현:(1)발아후비발아전신강사동청IAA함량강저솔위77.80%,ABA함량강저솔위98.90%;몽고사동청IAA함량강저솔위75.80%,ABA함량강저솔위66.20%;2충사동청발아전후GA_3함량변화불대.(2)수Pb~(2+)농도적제고(20~1 500 mg·L~(-1)),IAA함량강저십분현저,고농도(대우1 000 mg·L~(-1))Pb~(2+)협박대GA_3함량재유영향,ABA함량몰유규률성적변화;재Pb~(2+)협박하,몽점사동청중3충내원격소함량균고우신강사동청.(3)사동청유묘중Pb~(2+)분포특정균위근>경>협.(4)고농도Pb~(2+)협박대사동청협편세포산생료불가역적상해.연구결과위진일보연구황막지구특유적상록활협관목사동청적생장특정화항역궤제제공료가삼고적의거.
The changes in three endogenous hormones,phytohormones gibberrelic acid (GA_3),indoles-3-acid (IAA) and abscisic acid (ABA),were studied around germination and under tress of different density of Pb~(2+) between two species of Ammopiptanathus.It was found that (1) around germination,in Xinjiang Ammopiptanthus the rate IAA decreased 77.80%,and the rate of ABA decreased 98.90%;and in Mongolia Ammopiptanthus the rate of IAA decreased 75.80%,the rate of ABA decreased 66.20%,and the GA_3 contents in both had no big change.(2) With the increase in Pb~(2+) concentration (20-1 500 mg·L~(-1)),the IAA decreased significantly;and only under the high density (more than 1 000 mg·L~(-1)) of Pb~(2+),the GA_3 was affected by it;the ABA did not change regularly.So the three endogenous hormone levels of Mongolia Ammopiptanthus were higher than those of Xinjiang Ammopiptanthus.(3)the distributions of Pb~(2+) in Ammopiptanthus seedlings are the root>stems>leaves.(4) the stress of high concentration of Pb~(2+) damages irreversibly the leaf cells of Ammopiptanthus.This study can provide the basis of reference data for further research on the growth characteristics,resilience and the mechanism of specific broadleaved evergreen shrubs-Amrnopiptanthus in desertification area.