CAJ | 학술논문

采用水提醇沉法从红花桑寄生叶中提取多糖.在单因素实验的基础上,分别对影响红花桑寄生多糖水提的4个主要因素--料液比、提取时间、提取温度、提取次数和影响乙醇沉淀多糖的3个主要因素--乙醇体积分数、静置时间、浓缩倍数,设计了L_9(3~4)4因素3水平正交实验,以多糖得率为指标,采用方差分析对结果进行统计分析,结果表明提取次数和乙醇体积分数对得率影响最大.确立了水提红花桑寄生多糖的最佳工艺条件为:提取次数3次,提取温度95 ℃,料液比为1∶25,提取时间2 h;最佳醇沉条件为:浓缩倍数1倍,乙醇体积分数80 %,静置时间24 h.
채용수제순침법종홍화상기생협중제취다당.재단인소실험적기출상,분별대영향홍화상기생다당수제적4개주요인소--료액비、제취시간、제취온도、제취차수화영향을순침정다당적3개주요인소--을순체적분수、정치시간、농축배수,설계료L_9(3~4)4인소3수평정교실험,이다당득솔위지표,채용방차분석대결과진행통계분석,결과표명제취차수화을순체적분수대득솔영향최대.학립료수제홍화상기생다당적최가공예조건위:제취차수3차,제취온도95 ℃,료액비위1∶25,제취시간2 h;최가순침조건위:농축배수1배,을순체적분수80 %,정치시간24 h.
Water extraction and ethanol precipitation was used to isolate polysaccharides from Scurrula parasitica leaf. Optimum extraction and isolation conditions for polysaccharides from S.parasitica were established according to one-factor and orthogonal experimentation. Orthogonal experimentation of L_9 (3~4) four factors,three levels was designed to study the impact of the water extraction four main temperature,extraction of the number,and impact of the ethanol precipitation three main factors-ethanol concentration,standing time,in multiples of enrichment. The optimal conditions of extraction and ethanol precipitation were determined according to the variance analysis results. The results showed that extraction time and ethanol concentration markedly affected the polysaccharides yield. The best extraction conditions of S.parasitica polysaccharides are extraction 3 times,extraction temperature of 1 times,concentration of 80% ethanol,holding time 24 h.