CAJ | 학술논문

目的:建立当归流浸膏的质量控制标准.方法:对当归流浸膏中阿魏酸和藁本内酯采用薄层色谱法进行定性鉴别,采用HPLC梯度洗脱法进行定量检测,色谱条件为Diamonsil ODS C_(18)柱(250 mm×4.6 mm,5 μm),流动相A-甲醇(含0.4%冰醋酸),B-0.4%冰醋酸水溶液,梯度洗脱(0～15 min,38%A;15～20 min,38%～70%A;20～40 min,70%A;40～45 min,70%～38%A),检测波长为323 nm,流速为1.0 mL/min,柱温为35℃.结果:阿魏酸和藁本内酯分别在1.008～10.08 μg/mL和9.985～99.85 μg/mL范围内线性关系良好,平均回收率分别为98.11%和101.61%,RSD值分别为1.58%和1.32%.结论:该方法简便易行,具有良好的重现性和稳定性,可用于当归流浸膏的质量控制.
목적:건립당귀류침고적질량공제표준.방법:대당귀류침고중아위산화고본내지채용박층색보법진행정성감별,채용HPLC제도세탈법진행정량검측,색보조건위Diamonsil ODS C_(18)주(250 mm×4.6 mm,5 μm),류동상A-갑순(함0.4%빙작산),B-0.4%빙작산수용액,제도세탈(0～15 min,38%A;15～20 min,38%～70%A;20～40 min,70%A;40～45 min,70%～38%A),검측파장위323 nm,류속위1.0 mL/min,주온위35℃.결과:아위산화고본내지분별재1.008～10.08 μg/mL화9.985～99.85 μg/mL범위내선성관계량호,평균회수솔분별위98.11%화101.61%,RSD치분별위1.58%화1.32%.결론:해방법간편역행,구유량호적중현성화은정성,가용우당귀류침고적질량공제.
AIM: To establish the quality standards for Chinese extractum angelicae liquidum. METHODS: TLC was used to identify ferulic acid and ligustilide and HPLC to determine the content of ferulic acid and ligustil-ide. HPLC was performed on a Diamonsil ODS-C_(18) analytical column(250 mm×4.6 mm, 5 μm) with gradient elu-tion(0-15 min, 38%A; 15-20 min, 38%A-70%A; 20-40 min, 70%A; 40-45 min, 70%A-38%A) of methanol (A, containing 0.4% glacial acetic) and 0.4% glacial acetic acid(B) at the flow rate of 1.0 mL/min. The diode array detection wavelength was set at 323 nm and the column temperature was at 35℃. RESULTS: The linear range of ferulic acid and ligustilide were from 1.008 μg/mL to 10.08 μg/mL and 9.985 μg/mL to 99.85 μg/mL,the average recoveries of both were 98.11% and 101.61%, RSD were 1.58% and 1.32%. CONCLUSION: The method is rapid, simple and accurate with high reproducibility and can be used to control the quality of Chinese extractum angelicae liquidum.