中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2012年
10期
816-820
,共5页
王祖森%吴力群%李玉军%姚如勇%伊鑫%耿超%胡维昱%韩冰
王祖森%吳力群%李玉軍%姚如勇%伊鑫%耿超%鬍維昱%韓冰
왕조삼%오력군%리옥군%요여용%이흠%경초%호유욱%한빙
癌,肝细胞%甲胎蛋白类%连接蛋白43%肿瘤复发,局部
癌,肝細胞%甲胎蛋白類%連接蛋白43%腫瘤複髮,跼部
암,간세포%갑태단백류%련접단백43%종류복발,국부
Carcinoma,hepatocellular%Alpha-fetoprotein%Connexin 43%Neoplasm recurrence,local
目的 研究Cx43、CD105、VEGF在不同血清AFP(α-fetoprotein)浓度的乙型肝炎病毒性肝细胞癌(hepatocellular carcinoma,HCC)组织中的表达的关系以及Cx43的表达和RO切除术后复发和预后的关系.方法 应用组织芯片技术和免疫组织化学PV-6000两步法检测234例HBV相关HCC组织标本Cx43和CD105、VEGF的表达,应用RT-PCR检测20例HBV相关HCC标本Cx43mRNA的表达,并分析在不同血清AFP浓度下与Cx43的表达与CD105、VEGF的表达的关联以及与HCC患者根治切除术后的复发和预后的关系. 结果 免疫组织化学法和RT-PCR均检测到Cx43在HCC组织中表达,AFP＜ 400 μg/L的HBV相关HCC组织中Kaplan-Meier分析显示Cx43阳性表达者其1年复发、无瘤生存和总体生存均显著低于阴性表达组(Log Rank P =0.001,0.026,0.000),Cox回归分析表明Cx43阳性表达是HCC早期复发和预后(Log Rank P=0.049、0.035)的独立影响因子,Cx43阳性表达者CD105、VEGF低表达(P=0.018、0.023),并与组织分化程度(P =0.002),病灶数(P =0.033)血管癌栓(P =0.029)有关.结论 Cx43的表达与CD105、VEGF的表达相关,在AFP＜400 μg/L的HBV相关HCC的患者中Cx43阴性表达预示早期复发和不良预后.
目的 研究Cx43、CD105、VEGF在不同血清AFP(α-fetoprotein)濃度的乙型肝炎病毒性肝細胞癌(hepatocellular carcinoma,HCC)組織中的錶達的關繫以及Cx43的錶達和RO切除術後複髮和預後的關繫.方法 應用組織芯片技術和免疫組織化學PV-6000兩步法檢測234例HBV相關HCC組織標本Cx43和CD105、VEGF的錶達,應用RT-PCR檢測20例HBV相關HCC標本Cx43mRNA的錶達,併分析在不同血清AFP濃度下與Cx43的錶達與CD105、VEGF的錶達的關聯以及與HCC患者根治切除術後的複髮和預後的關繫. 結果 免疫組織化學法和RT-PCR均檢測到Cx43在HCC組織中錶達,AFP＜ 400 μg/L的HBV相關HCC組織中Kaplan-Meier分析顯示Cx43暘性錶達者其1年複髮、無瘤生存和總體生存均顯著低于陰性錶達組(Log Rank P =0.001,0.026,0.000),Cox迴歸分析錶明Cx43暘性錶達是HCC早期複髮和預後(Log Rank P=0.049、0.035)的獨立影響因子,Cx43暘性錶達者CD105、VEGF低錶達(P=0.018、0.023),併與組織分化程度(P =0.002),病竈數(P =0.033)血管癌栓(P =0.029)有關.結論 Cx43的錶達與CD105、VEGF的錶達相關,在AFP＜400 μg/L的HBV相關HCC的患者中Cx43陰性錶達預示早期複髮和不良預後.
목적 연구Cx43、CD105、VEGF재불동혈청AFP(α-fetoprotein)농도적을형간염병독성간세포암(hepatocellular carcinoma,HCC)조직중적표체적관계이급Cx43적표체화RO절제술후복발화예후적관계.방법 응용조직심편기술화면역조직화학PV-6000량보법검측234례HBV상관HCC조직표본Cx43화CD105、VEGF적표체,응용RT-PCR검측20례HBV상관HCC표본Cx43mRNA적표체,병분석재불동혈청AFP농도하여Cx43적표체여CD105、VEGF적표체적관련이급여HCC환자근치절제술후적복발화예후적관계. 결과 면역조직화학법화RT-PCR균검측도Cx43재HCC조직중표체,AFP＜ 400 μg/L적HBV상관HCC조직중Kaplan-Meier분석현시Cx43양성표체자기1년복발、무류생존화총체생존균현저저우음성표체조(Log Rank P =0.001,0.026,0.000),Cox회귀분석표명Cx43양성표체시HCC조기복발화예후(Log Rank P=0.049、0.035)적독립영향인자,Cx43양성표체자CD105、VEGF저표체(P=0.018、0.023),병여조직분화정도(P =0.002),병조수(P =0.033)혈관암전(P =0.029)유관.결론 Cx43적표체여CD105、VEGF적표체상관,재AFP＜400 μg/L적HBV상관HCC적환자중Cx43음성표체예시조기복발화불량예후.
Objective To study the expressions of Cx43,CD105 and VEGF in HBV related HCC tissues and the relationships between Cx43 expression and recurrence and prognosis after cancer radical resection in HCC patients stratified by serum AFP levels. Methods The expressions of Cx43,CD105,VEGF in 234 HBV related HCC tissues were examined by tissue microarray and two-step methods of PV-6000 of immunohistochemistry and the expressions of Cx43 in 20 frozen HCC specimens were examined by RT-PCR. Results Cx43 in HCC tissues was positive as examined by both immunohistochemistry and RTPCR methods.Positive Cx43 expression is correlated with lower early recurrence ( Log Rank P =0.001 ),longer disease free survival (Log Rank P =0.026 ) and overall survival( Log Rank P =0.000 ) as showed by the Kaplan-Meier analysis in patients with AFP ＜ 400 μg/L. The expression of Cx43 is an independent prognostic factor.The positive expression of Cx43 related with lower positive expression of CD105 and VEGF (P =0.018,0.023 ),and correlated with histological differentiation (P =0.002),the number of focus (P =0.033 ),blood vessel tumor embolism ( P =0.029 ). Conclusions The expression of Cx43 is correlative with the expression of CD105 and VEGF,and is predictive of HCC early recurrence and poor prognosis after radical hepatectomy in HBV related HCC patients with serum AFP ＜ 400 μg/L.
