中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2012年
5期
296-300
,共5页
龋齿%显微镜检查,共焦%显微镜检查,电子,扫描%微隙
齲齒%顯微鏡檢查,共焦%顯微鏡檢查,電子,掃描%微隙
우치%현미경검사,공초%현미경검사,전자,소묘%미극
Dental caries%Microscopy,confocal%Microscopy,electron,transmission%Gap
目的 体外建立经变形链球菌致龋的人工龋模型,评价牙与充填体间微隙大小对微生物致龋的影响.方法 用无龋磨牙制备的牙块和形态大小相同的复合树脂块制作二者间隙分别为0、25、50、100、190及250 μm的6个实验组样本,对照组样本使用粘接剂粘接牙面和复合树脂,6个实验组及对照组每组各8个样本,共56个样本经14 d变形链球菌连续培养后切片,采用激光共聚焦扫描显微镜( confocal laser scanning microscope,GLSM)检测牙体洞外表面龋损及洞壁龋损的脱矿程度,分别测定龋损最大深度、最大长度、荧光面积及平均荧光量等参数并进行统计学分析；扫描电镜观察界面微隙内沉积物与龋损形成情况.结果 CLSM和扫描电镜观察显示,除2个对照组样本未形成洞壁龋损外其余样本均形成了不同程度的洞外表面龋损和洞壁龋损；各组表面龋损脱矿程度差异均无统计学意义(P＞0.05).190 μm组及250 μm组洞壁龋损最大深度[分别为(1145.37±198.98)、(1190.12±290.80)μm]、最大长度、荧光面积及平均荧光度均显著高于0、25、50及100 μm组[龋损最大深度分别为( 205.25±122.61)、(303.87±118.80)、(437.75±154.88)、(602.87±269.13)μm],差异均有统计学意义(P＜0.01),脱矿程度随微隙增加而加重；而0、25、50μn组洞壁龋损最大深度、最大长度及荧光面积差异均无统计学意义.结论 体外牙与充填体间的微隙大于100 μm时提示洞壁龋损有明显进展；微隙的存在是影响牙与充填体间微生物致龋的重要因素.
目的 體外建立經變形鏈毬菌緻齲的人工齲模型,評價牙與充填體間微隙大小對微生物緻齲的影響.方法 用無齲磨牙製備的牙塊和形態大小相同的複閤樹脂塊製作二者間隙分彆為0、25、50、100、190及250 μm的6箇實驗組樣本,對照組樣本使用粘接劑粘接牙麵和複閤樹脂,6箇實驗組及對照組每組各8箇樣本,共56箇樣本經14 d變形鏈毬菌連續培養後切片,採用激光共聚焦掃描顯微鏡( confocal laser scanning microscope,GLSM)檢測牙體洞外錶麵齲損及洞壁齲損的脫礦程度,分彆測定齲損最大深度、最大長度、熒光麵積及平均熒光量等參數併進行統計學分析；掃描電鏡觀察界麵微隙內沉積物與齲損形成情況.結果 CLSM和掃描電鏡觀察顯示,除2箇對照組樣本未形成洞壁齲損外其餘樣本均形成瞭不同程度的洞外錶麵齲損和洞壁齲損；各組錶麵齲損脫礦程度差異均無統計學意義(P＞0.05).190 μm組及250 μm組洞壁齲損最大深度[分彆為(1145.37±198.98)、(1190.12±290.80)μm]、最大長度、熒光麵積及平均熒光度均顯著高于0、25、50及100 μm組[齲損最大深度分彆為( 205.25±122.61)、(303.87±118.80)、(437.75±154.88)、(602.87±269.13)μm],差異均有統計學意義(P＜0.01),脫礦程度隨微隙增加而加重；而0、25、50μn組洞壁齲損最大深度、最大長度及熒光麵積差異均無統計學意義.結論 體外牙與充填體間的微隙大于100 μm時提示洞壁齲損有明顯進展；微隙的存在是影響牙與充填體間微生物緻齲的重要因素.
목적 체외건립경변형련구균치우적인공우모형,평개아여충전체간미극대소대미생물치우적영향.방법 용무우마아제비적아괴화형태대소상동적복합수지괴제작이자간극분별위0、25、50、100、190급250 μm적6개실험조양본,대조조양본사용점접제점접아면화복합수지,6개실험조급대조조매조각8개양본,공56개양본경14 d변형련구균련속배양후절편,채용격광공취초소묘현미경( confocal laser scanning microscope,GLSM)검측아체동외표면우손급동벽우손적탈광정도,분별측정우손최대심도、최대장도、형광면적급평균형광량등삼수병진행통계학분석；소묘전경관찰계면미극내침적물여우손형성정황.결과 CLSM화소묘전경관찰현시,제2개대조조양본미형성동벽우손외기여양본균형성료불동정도적동외표면우손화동벽우손；각조표면우손탈광정도차이균무통계학의의(P＞0.05).190 μm조급250 μm조동벽우손최대심도[분별위(1145.37±198.98)、(1190.12±290.80)μm]、최대장도、형광면적급평균형광도균현저고우0、25、50급100 μm조[우손최대심도분별위( 205.25±122.61)、(303.87±118.80)、(437.75±154.88)、(602.87±269.13)μm],차이균유통계학의의(P＜0.01),탈광정도수미극증가이가중；이0、25、50μn조동벽우손최대심도、최대장도급형광면적차이균무통계학의의.결론 체외아여충전체간적미극대우100 μm시제시동벽우손유명현진전；미극적존재시영향아여충전체간미생물치우적중요인소.
Objective To evaluate the effect of gap size between tooth and restorative materials on microbiolism based caries in vitro.Methods Tooth blocks made of human molars without caries and the same size composite resin blocks were selected and prepared.Tooth-resin matrix was mounted on resin base with a gap size of 0,25,50,100,190,250 μm and a control group was dealed with adhesive system.Six experimental groups and one control group were included,with 8 samples in one group and a total of 56 samples.The samples were cultured by a 14-day sequential batch culture technique.The development of outer surface lesion and wall lesion was assessed with confocal laser scanning microscope (CLSM) by measuring the maximum lesion depth,fluorescence areas and average fluorescence value.The data were collected and statistically analyzed.The deposits of the tooth-restoration interface and the development of the carious lesion were observed by scanning electron microscope( SEM ).Results Most groups showed outer surface lesion and wall surface lesions observed by CLSM and SEM except 2 samples in control group.There was no significant difference on the outer surface lesion (P ＞ 0.05 ).The maximum lesion depth [ ( 1145.37 + 198.98),( 1190.12 ± 290.80) μm respectively],the maximum lesion length,fluorescence areas and average fluorescence value of 190 and 250 μm groups' wall lesions were significantly higher than the0,25,50 and 100 μm groups[ the maximum lesion depth was (205.25 ± 122.61 ),(303.87 ±118.80),(437.75 ± 154.88),(602.87 ± 269.13 ) μm respectively],P ＜0.01.With the increase of the gap size,the demineralization developed more seriously.While the maximum lesion depth,the maximum lesion length and fluorescence areas of 0,25,50 μm groups' wall lesions were of no significant difference.Conclusions There was close relationship between gap size and wall lesion when the gap was above 100 μmat tooth-composite resin interface.The existence of gap was the main influencing factor on the development of microbiolism based caries lesion.
