CAJ | 학술논문

目的探讨IL-6、IL-6膜受体(IL-6R)及其下游信号转导相关分子在颞叶癫痫(TLE)发生中的作用. 方法选择自2010年1月至2010年12月在第三军医大学新桥医院神经外科行手术治疗的TLE患者40例(TLE组),同期行手术治疗的外伤、高血压脑出血等患者40例(对照组)；收集其手术过程中切除的颞叶组织.利用RT-PCR、ELISA等方法分析IL-6、IL-6R、sIL-6R以及sgp130的mRNA或蛋白水平的表达变化情况. 结果 TLE组和对照组中均可检测到IL-6和IL-6R mRNA的表达,但TLE组致病灶中IL-6和IL-6R mRNA水平均显著高于对照组,差异有统计学意义(P＜0.05).ELISA结果提示,sIL-6R在TLE组致痫灶中的表达量与对照组比较明显增高,差异有统计学意义(P＜0.05);sgpl30的含量略高于对照组,差异没有统计学意义(P＞0.05). 结论局部高浓度的IL-6通过经典信号转导或者跨信号转导机制作用于TLE病灶细胞,影响这些细胞的生物学功能,参与癫痫发生过程.
목적 탐토IL-6、IL-6막수체(IL-6R)급기하유신호전도상관분자재섭협전간(TLE)발생중적작용. 방법 선택자2010년1월지2010년12월재제삼군의대학신교의원신경외과행수술치료적TLE환자40례(TLE조),동기행수술치료적외상、고혈압뇌출혈등환자40례(대조조)；수집기수술과정중절제적섭협조직.이용RT-PCR、ELISA등방법분석IL-6、IL-6R、sIL-6R이급sgp130적mRNA혹단백수평적표체변화정황. 결과 TLE조화대조조중균가검측도IL-6화IL-6R mRNA적표체,단TLE조치병조중IL-6화IL-6R mRNA수평균현저고우대조조,차이유통계학의의(P＜0.05).ELISA결과제시,sIL-6R재TLE조치간조중적표체량여대조조비교명현증고,차이유통계학의의(P＜0.05);sgpl30적함량략고우대조조,차이몰유통계학의의(P＞0.05). 결론 국부고농도적IL-6통과경전신호전도혹자과신호전도궤제작용우TLE병조세포,영향저사세포적생물학공능,삼여전간발생과정.
Objective To study the expression levels ofinterlenkin-6 (IL-6), IL-6 receptor (IL-6R),its soluble IL-6 receptor (sIL-6R) and soluble glycoprotein 130 (sgp130) in brain tissues of refractory temporal lobe epilepsy (TLE) and explore their role in TLE. Methods Forty patients with TLE and other 40 patients with trauma/hypertensive cerebral hemorrhage (controls), admitted to and performed surgery in our hospital from January 2010 to December 2010,were chosen in our study.Brain tissues from temporal lobe were collected during the surgery.By using real time-PCR and ELISA,the mRNA or protein expression levels of IL-6,IL-6R,sIL-6R and sgp130 were analyzed. Results The mRNA expressions of IL-6 and IL-6R in the TLE brain tissues were significantly higher as compared with those in controls (P＜0.05).ELISA indicated that sIL-6R level in the TLE brain tissues was significantly higher than those in controls (P＜0.05); however,sgp130 level in the TLE brain tissues was slightly higher than those in controls without significant difference (P＞0.05). Conclusion The high level of IL-6 may influence the function of varies types of cells within the epileptic foci of patients with TLE through classical signaling pathway or trans-signaling pathway, which is potentially involved in the epileptogenesis of TLE.