背景:随着机体衰老性腺功能逐渐降低,中国传统医学认为精气虚弱是导致机体衰老的主要原因.
目的:观察中药天年饮对亚急性衰老大鼠血清睾酮含量、睾丸组织超氧化物歧化酶的活性及睾丸生精细胞凋亡的影响.
设计:随机对照实验.
单位:承德医学院人体解剖学教研室.
材料:实验于2003-04/07在承德医学院基础医学研究所进行.选择成年雄性SD大鼠40只,随机分为4组,每组10只.模型组、用药组、对照组采用D-半乳糖连续腹腔注射200mg/(kg·d)制备亚急性衰老动物模型,1次/d,共40 d,模型组大鼠于造模成功后不再作任何处理.用药组大鼠于造模成功后每天用天年饮灌胃(天年饮由灸何首乌、怀牛膝、肉从蓉、茯苓、丹参、淫羊藿6味中药组成,经熬制后含生药1.5 g/mL),1次/d,共30 d;对照组大鼠在造模成功后每天灌胃同等剂量的生理盐水,时间同用药组大鼠.方法:各组大鼠血清中睾酮的含量采用酶联免疫吸附方法检测,睾丸组织中超氧化物歧化酶的活性采用黄嘌呤氧化酶法检测,睾丸生精细胞的凋亡情况采用免疫组化法观察.
主要观察指标:①各组大鼠血清中睾酮的含量.②睾丸组织中超氧化物歧化酶的活性.③睾丸生精细胞的凋亡情况.
结果:40只大鼠均进入结果分析.①血清中睾酮的含量:模型组大鼠血清睾酮含量低于正常组[(0.52±0.15)μg/L,(1.26±0.32)μg/L,(t=3.004,P＜0.05)],用药组高于模型组[(1.16±0.32)μg/L,(0.52±0.15)μg/L,(t=2.321,P＜0.05)].②睾丸组织超氧化物歧化酶的活性:模型组明显低于正常组[(107.22±9.33)kNU/g,(147.73±11.9)kNU/g,(t=13.339,P＜0.01)],用药组明显高于模型组[(141.05±14.57)kNU/g,(107.22±9.33)kNU/g,(t=9.970,P＜0.01)].③生精细胞的凋亡情况:模型组凋亡生精小管百分率、凋亡阳性细胞率均明显高于正常组[(53.95±2.02)%比(34.21±2.10)%,(8.67±0.80)%比(3.86±0.52)%,(x2=7.9,＜0.01,x25.01.P＜0.05)];用药组明显低于模型组[(35.33±2.18)%比(53.95±2.02)%,(4.68±0.74)%比(8.67±0.80)%,(x2=7.02,P＜0.01,x2=3.96,P＜0.05)].
结论:衰老模型睾丸生精细胞凋亡增多,经天年饮灌胃后可以使凋亡细胞阳性率明显下降,说明天年饮可以清除体内过多的氧自由基,减少凋亡发生,改善动物模型的生精功能.
揹景:隨著機體衰老性腺功能逐漸降低,中國傳統醫學認為精氣虛弱是導緻機體衰老的主要原因.
目的:觀察中藥天年飲對亞急性衰老大鼠血清睪酮含量、睪汍組織超氧化物歧化酶的活性及睪汍生精細胞凋亡的影響.
設計:隨機對照實驗.
單位:承德醫學院人體解剖學教研室.
材料:實驗于2003-04/07在承德醫學院基礎醫學研究所進行.選擇成年雄性SD大鼠40隻,隨機分為4組,每組10隻.模型組、用藥組、對照組採用D-半乳糖連續腹腔註射200mg/(kg·d)製備亞急性衰老動物模型,1次/d,共40 d,模型組大鼠于造模成功後不再作任何處理.用藥組大鼠于造模成功後每天用天年飲灌胃(天年飲由灸何首烏、懷牛膝、肉從蓉、茯苓、丹參、淫羊藿6味中藥組成,經熬製後含生藥1.5 g/mL),1次/d,共30 d;對照組大鼠在造模成功後每天灌胃同等劑量的生理鹽水,時間同用藥組大鼠.方法:各組大鼠血清中睪酮的含量採用酶聯免疫吸附方法檢測,睪汍組織中超氧化物歧化酶的活性採用黃嘌呤氧化酶法檢測,睪汍生精細胞的凋亡情況採用免疫組化法觀察.
主要觀察指標:①各組大鼠血清中睪酮的含量.②睪汍組織中超氧化物歧化酶的活性.③睪汍生精細胞的凋亡情況.
結果:40隻大鼠均進入結果分析.①血清中睪酮的含量:模型組大鼠血清睪酮含量低于正常組[(0.52±0.15)μg/L,(1.26±0.32)μg/L,(t=3.004,P＜0.05)],用藥組高于模型組[(1.16±0.32)μg/L,(0.52±0.15)μg/L,(t=2.321,P＜0.05)].②睪汍組織超氧化物歧化酶的活性:模型組明顯低于正常組[(107.22±9.33)kNU/g,(147.73±11.9)kNU/g,(t=13.339,P＜0.01)],用藥組明顯高于模型組[(141.05±14.57)kNU/g,(107.22±9.33)kNU/g,(t=9.970,P＜0.01)].③生精細胞的凋亡情況:模型組凋亡生精小管百分率、凋亡暘性細胞率均明顯高于正常組[(53.95±2.02)%比(34.21±2.10)%,(8.67±0.80)%比(3.86±0.52)%,(x2=7.9,＜0.01,x25.01.P＜0.05)];用藥組明顯低于模型組[(35.33±2.18)%比(53.95±2.02)%,(4.68±0.74)%比(8.67±0.80)%,(x2=7.02,P＜0.01,x2=3.96,P＜0.05)].
結論:衰老模型睪汍生精細胞凋亡增多,經天年飲灌胃後可以使凋亡細胞暘性率明顯下降,說明天年飲可以清除體內過多的氧自由基,減少凋亡髮生,改善動物模型的生精功能.
배경:수착궤체쇠로성선공능축점강저,중국전통의학인위정기허약시도치궤체쇠로적주요원인.
목적:관찰중약천년음대아급성쇠로대서혈청고동함량、고환조직초양화물기화매적활성급고환생정세포조망적영향.
설계:수궤대조실험.
단위:승덕의학원인체해부학교연실.
재료:실험우2003-04/07재승덕의학원기출의학연구소진행.선택성년웅성SD대서40지,수궤분위4조,매조10지.모형조、용약조、대조조채용D-반유당련속복강주사200mg/(kg·d)제비아급성쇠로동물모형,1차/d,공40 d,모형조대서우조모성공후불재작임하처리.용약조대서우조모성공후매천용천년음관위(천년음유구하수오、부우슬、육종용、복령、단삼、음양곽6미중약조성,경오제후함생약1.5 g/mL),1차/d,공30 d;대조조대서재조모성공후매천관위동등제량적생리염수,시간동용약조대서.방법:각조대서혈청중고동적함량채용매련면역흡부방법검측,고환조직중초양화물기화매적활성채용황표령양화매법검측,고환생정세포적조망정황채용면역조화법관찰.
주요관찰지표:①각조대서혈청중고동적함량.②고환조직중초양화물기화매적활성.③고환생정세포적조망정황.
결과:40지대서균진입결과분석.①혈청중고동적함량:모형조대서혈청고동함량저우정상조[(0.52±0.15)μg/L,(1.26±0.32)μg/L,(t=3.004,P＜0.05)],용약조고우모형조[(1.16±0.32)μg/L,(0.52±0.15)μg/L,(t=2.321,P＜0.05)].②고환조직초양화물기화매적활성:모형조명현저우정상조[(107.22±9.33)kNU/g,(147.73±11.9)kNU/g,(t=13.339,P＜0.01)],용약조명현고우모형조[(141.05±14.57)kNU/g,(107.22±9.33)kNU/g,(t=9.970,P＜0.01)].③생정세포적조망정황:모형조조망생정소관백분솔、조망양성세포솔균명현고우정상조[(53.95±2.02)%비(34.21±2.10)%,(8.67±0.80)%비(3.86±0.52)%,(x2=7.9,＜0.01,x25.01.P＜0.05)];용약조명현저우모형조[(35.33±2.18)%비(53.95±2.02)%,(4.68±0.74)%비(8.67±0.80)%,(x2=7.02,P＜0.01,x2=3.96,P＜0.05)].
결론:쇠로모형고환생정세포조망증다,경천년음관위후가이사조망세포양성솔명현하강,설명천년음가이청제체내과다적양자유기,감소조망발생,개선동물모형적생정공능.
BACKGROUND: In follow of the gradual decrease of senile gonad function in the body, traditional Chinese medicine views that deficiency of essential qi is the chief reason of body senility.
OBJECTIVE: To observe the effects of tian nian yin on serum testosterone content, superoxide dismutase activity in testicular tissue and apoptosis of testicular seminiferous cell in subacute senile rats.
DESIGN: Randomized controlled experiment.
SETTING: Human Anatomy Department of Chengde Medical College.
MATERIALS: The experiment was performed in Institute of Basic Medicine of Chengde Medical College from April to July 2003, in which,40 SD male rats were employed and randomized into 4 groups, 10 rats in each group. In model group, medicinal group and the control, D-galactose was injected 200 mg/(kg·d) to prepare subacute senile animal model, once a day, continuously for 40 days. No any management was given in model
group after modeling success. In medicinal group, gastric infusion with tian nian yin [composed of zhi heshouwu (Radix Polygoni Mutiflori Preparatea),huainiuxi (Radix Achyranthis Bidentatae), roucongrong (Herba Cistanchis),fuling (Poria), danshen (Radix Salviae Miltiorrhizae) and yinyanghuo (Herba Epimedii), it was decocted till 1.5 g/mL with raw herbs contained] was administrated once a day, totally for 30 days after modeling success. In the control, gastric infusion was administrated with physiological saline of same usage as the medicinal group after modeling success.
INTERVENTIONS: In every group, enzyme linked immunosorbent assay (ELISA) was used to determine testosterone content in serum. Xanthine oxidase assay (XOD) was used to determine superoxide dismutase activity in testicular tissue and immunohistochemical method (IHC) was used to observe apoptosis of testicular seminiferous cell.
MAIN OUTCOME MEASURES: ① Testosterone content in serum. ②Superoxide dismutase activity in testicular tissue. ③ Apoptosis of testicular seminiferous cell
RESULTS: Forty rats all entered result analysis. ① Testosterone content in serum: Testosterone content in model group was lower than normal group [(0.52±0.15) μg/L, (1.26 0.32) μg/L, (t=3.004, P ＜ 0.05)], and that in medicinal group was higher than model group [(1.16±0.32) μg/L,(0.52 0.15) μg/L, (t=2.321, P ＜ 0.05)]. ② Superoxide dismutase activity in testicular tissue: Superoxide dismutase activity in model group was lower remarkably than the normal group [(107.22±9.33) kNU/g, (147.73±11.90) kNU/g,(t=13.339, P ＜ 0.01)], and that in medicinal group was higher remarkably
than model group [(141.05±14.57) kNU/g, (107.22±9.33) kNU/g, (t=9.970,P ＜ 0.01)]. ③ Apoptosis of testicular seminiferous cell: Percentage of apoptotic seniniferous tubule and apoptotic rate of positive cell in model group were higher remarkably thannormal group respectively [(53.95 ±2.02)％compared with (34.21±2.10)％, (8.67±0.80)％ compared with (3.86±0.52)％,(x2=7.9, P ＜ 0.01, x2=5.01, P ＜ 0.05)], and those in medicinal group were lower remarkably than model group[(35.33±2.18)％ compared with (53.95±2.02)％, (4.68±0.74)％ compared with (8.67±0.80)％, (x2=7.02, P ＜ 0.01, x2=3.96, P ＜ 0.05)].
CONCLUSION: The apoptosis of testicular seminiferous cell was increased in senile modelgroup. Tian nian yin in gastric infusion decreased apoptotic rate of positive cell. It is explained that tian nian yin eliminates surplus oxygenic free radical, decreases apoptosis and improves seminiferous function in animal model.
