CAJ | 학술논문

为研究棉花GA20-氧化酶同源基因GhGA20ox1的功能,将该基因转入本明烟(N.benthamiana)中进行超量表达.RT-PCR分析表明GhGA20ox1基因在转基因植株中得到了不同水平的表达.GhGA20ox1基因的超量表达促进了本明烟中的GA4+7合成,并导致赤霉素过量的表型出现.转基因本明烟的表型变化程度与GhGA20ox1基因的表达水平和GA4+7的含量一致.这些结果表明,GhGA20ox1基因编码一个有功能的GA 20-氧化酶,能够在转基因烟草中促进活性GA(GA4+7)的合成,可以用作目的基因来提高棉花纤维和其他植物的内源GA水平.
위연구면화GA20-양화매동원기인GhGA20ox1적공능,장해기인전입본명연(N.benthamiana)중진행초량표체.RT-PCR분석표명GhGA20ox1기인재전기인식주중득도료불동수평적표체.GhGA20ox1기인적초량표체촉진료본명연중적GA4+7합성,병도치적매소과량적표형출현.전기인본명연적표형변화정도여GhGA20ox1기인적표체수평화GA4+7적함량일치.저사결과표명,GhGA20ox1기인편마일개유공능적GA 20-양화매,능구재전기인연초중촉진활성GA(GA4+7)적합성,가이용작목적기인래제고면화섬유화기타식물적내원GA수평.
To determine the physiological function of GhGA20ox1, a homologous gene of GA 20-oxidase from elongating cotton fibers, we expressed this gene ectopically in Nicotiana benthamiana. Reverse transcription-PCR analysis showed that the GhGA20ox1 gene was expressed in the transgenic plants at various levels. It was demonstrated that overexpression of GhGA20ox1 enhanced preferentially the GA4+7 biosynthesis in N. benthamiana and conferred GA-overproduction characters to transformants. The extent of phenotypic alteration in the transgenic plants was found to correlate with the transcriptional levels of GhGA20ox1 and GA contents. Results indicated that the GhGA20ox1 gene promoted the biosynthesis of the active GAs (GA4+7) in transgenic tobacco plants therefore represents a useful gene for manipulating GA levels.