CAJ | 학술논문

采用聚丙烯酰胺垂直电泳技术(PAGE)对来自山东野生、江苏野生、浙江养殖、广西野生白色壳4个文蛤Meretrix meretrix群体两种组织(消化腺、闭壳肌)的EST、MDH、ME、ADH、SOD、CAT等6种同工酶进行了分析.结果显示:文蛤不同组织之间、不同群体之间、养殖群体和野生群体之间的同工酶酶谱均有较明显的差别;广西白壳群体的多种同工酶酶谱与其它几个群体相比有较大差异,说明该种群与另外3个群体种质差异较大,亲缘关系较远.对山东野生、广西普通壳色和广西白色壳色3个群体的4种同工酶(EST、AMY、MDH、SOD)进行了研究,结果表明:广西普通壳色群体和山东群体的酶谱相似,而与广西白色壳群体差异明显,初步推测广西白色壳群体为文蛤属的其他种类;在文蛤各群体中均找到了特征性酶带,可以作为区别于文蛤不同群体的蛋白标记,用于文蛤种质资源的分析鉴定,并可为选育优良品种文蛤提供遗传依据.
채용취병희선알수직전영기술(PAGE)대래자산동야생、강소야생、절강양식、엄서야생백색각4개문합Meretrix meretrix군체량충조직(소화선、폐각기)적EST、MDH、ME、ADH、SOD、CAT등6충동공매진행료분석.결과현시:문합불동조직지간、불동군체지간、양식군체화야생군체지간적동공매매보균유교명현적차별;엄서백각군체적다충동공매매보여기타궤개군체상비유교대차이,설명해충군여령외3개군체충질차이교대,친연관계교원.대산동야생、엄서보통각색화엄서백색각색3개군체적4충동공매(EST、AMY、MDH、SOD)진행료연구,결과표명:엄서보통각색군체화산동군체적매보상사,이여엄서백색각군체차이명현,초보추측엄서백색각군체위문합속적기타충류;재문합각군체중균조도료특정성매대,가이작위구별우문합불동군체적단백표기,용우문합충질자원적분석감정,병가위선육우량품충문합제공유전의거.
Vertical polyacrylamide gel electrophoresis(PAGE)technique was used to analyze six isozymes(esterase EST,malate dehydrogenase MDH,malic enzyme ME,alchol dehydrogenase ADH,superoxide dismutase SOD,catalase CAT)in adductor muscles and digestive glands of clam Meretrix meretrix in four populations(three natural populations from Shandongm(S),Jiangshu(J) and Guangxi(G,Gb), and hatched population from Wenzhou(Z)).The basic electrophoretic patterns of the 6 isozymes revealed that Gb population showed a significantly different isozymes phenotypes at EST, SOD, ME and MDH from the other population. The EST and AMY isozymes bands of Z was found to decrease compared with S, indicating that human activities may affect the genetic variation within and between populations. No significant differences in the isozymes phenotypes in SOD, AMY,CAT, ME, and MDH was observed between populations of S and G, but EST demonstrated a significantly different at isozyme phenotypes between S and G, showing distinct specific of geographical population.