CAJ | 학술논문

[目的]研究Pb~(2+)、Hg~(2+)对平菇菌丝生长的影响,探索元素Ca~(2+)、Mg~(2+)、Fe~(2+)与Hg~(2+)之间的拮抗效应.[方法]以平菇1012菌株为材料,采用含不同浓度梯度重金属及中微量元素的培养基进行平板培养试验,观察、测定菌丝生长情况.[结果]Pb~(2+)浓度为0.5～8.0 mg/L对平菇菌丝的生长没有不良影响,甚至有不显著的促进作用,Pb~(2+)浓度高于10.0 mg/L,抑制平菇菌丝的生长,Pb~(2+)浓度达到50.0 mg/L时,平菇菌丝的生长受到极显著抑制(P≤0.003),当Pb~(2+)浓度≥100.0 mg/L时,菌丝萌发后立即陷入停顿、死亡.Hg~(2+)对平菇的毒性强于Pb~(2+),从其低浓度开始就对平菇菌丝的生长表现抑制作用,当Hg~(2+)浓度为15 mg/L时,抑制作用显著 (P<0.05).向含浓度15 mg/L Hg~(2+)的平板培养基中分别添加碱土元素Ca~(2+)、Mg~(2+)和微量元素Fe~(2+),对Hg~(2+)抑制平菇菌丝生长的毒害有一定的拮抗解毒效果,其中又以添加浓度1 000 mg/L的Ca~(2+)效果最好.[结论]该研究对于控制食用菌重金属富集有重要的参考价值.
[목적]연구Pb~(2+)、Hg~(2+)대평고균사생장적영향,탐색원소Ca~(2+)、Mg~(2+)、Fe~(2+)여Hg~(2+)지간적길항효응.[방법]이평고1012균주위재료,채용함불동농도제도중금속급중미량원소적배양기진행평판배양시험,관찰、측정균사생장정황.[결과]Pb~(2+)농도위0.5～8.0 mg/L대평고균사적생장몰유불량영향,심지유불현저적촉진작용,Pb~(2+)농도고우10.0 mg/L,억제평고균사적생장,Pb~(2+)농도체도50.0 mg/L시,평고균사적생장수도겁현저억제(P≤0.003),당Pb~(2+)농도≥100.0 mg/L시,균사맹발후립즉함입정돈、사망.Hg~(2+)대평고적독성강우Pb~(2+),종기저농도개시취대평고균사적생장표현억제작용,당Hg~(2+)농도위15 mg/L시,억제작용현저 (P<0.05).향함농도15 mg/L Hg~(2+)적평판배양기중분별첨가감토원소Ca~(2+)、Mg~(2+)화미량원소Fe~(2+),대Hg~(2+)억제평고균사생장적독해유일정적길항해독효과,기중우이첨가농도1 000 mg/L적Ca~(2+)효과최호.[결론]해연구대우공제식용균중금속부집유중요적삼고개치.
[Objective]The research aimed to study the effects of Pb~(2+) and Hg~(2+) on mycelium growth of Pleurotus ostreatus and the antagonistic effects of them with alkaline-earth and trace elements.[Method]Taken P. ostreatus 1012 for materials and cultured with different culture media contained different concentrations heavy metals, alkaline-earth and trace elements.Then the growth situations were observed and determined.[Result]Pb~(2+) showed no inhibition or even insignificant improvement effects on the mycelium growth of P. ostreatus at low concentration (0.5-8.0 mg/L) until the concentration was higher than 10.0 mg/L.The mycelium growth of P. ostreatus was significantly inhibited since Pb~(2+) concentration ≥50.0 mg/L(P≤0.003).The mycelium stopped growing or died when Pb~(2+) concentration ≥100.0 mg/L. The toxicity of Hg~(2+) was higher than that of Pb~(2+), it restrained mycelium growth of P. ostreatus at low concentration, and showed significant inhibition (P<0.05) when Hg~(2+) concentration was 15 mg/L. When series concentrations of alkaline-earth elements Ca~(2+), Mg~(2+) and trace elements Fe~(2+) was added to medium contaminated by 15 mg/L Hg~(2+) respectively, their antagonistic effects on toxic action of Hg~(2+) was observed, and among Ca~(2+), Mg~(2+) and Fe~(2+), Ca~(2+) was the best since P. ostreatus blight induced by Hg~(2+) was totally rescued as 1 000 mg/L of Ca~(2+) added.[Conclusion]This study had important references value on the control of heavy metals enrichment in edible fungi.