CAJ | 학술논문

目的分析实验用西藏小型猪线粒体DNA控制区碱基序列变异分化类型,比较血液生理生化指标的差异,为实验动物新品种的培育奠定分子遗传学基础.方法采集59头西藏小型猪血液,分别进行全基因组DNA提取、线粒体DNA控制区的扩增、测序并分类,同时进行血液生理生化指标的测定和比较研究.结果根据串联重复区重复片段的变化,西藏小型猪线粒体DNA控制区分为A、B两种类型,分别占57.6%和42.4%,几乎与5'端序列在305、500和691的三个主要转换位点的变化相对应.A、B两类西藏小型猪18项血液生理生化指标统计结果显示,红细胞数(RBC)差异(P<0.01)有统计学意义,其他指标差异均无统计学意义.结论根据线粒体DNA控制区序列碱基变异,西藏小型猪分为两个类群,两个群体之间红细胞数存在明显差异.
목적 분석실험용서장소형저선립체DNA공제구감기서렬변이분화류형,비교혈액생리생화지표적차이,위실험동물신품충적배육전정분자유전학기출.방법 채집59두서장소형저혈액,분별진행전기인조DNA제취、선립체DNA공제구적확증、측서병분류,동시진행혈액생리생화지표적측정화비교연구.결과 근거천련중복구중복편단적변화,서장소형저선립체DNA공제구분위A、B량충류형,분별점57.6%화42.4%,궤호여5'단서렬재305、500화691적삼개주요전환위점적변화상대응.A、B량류서장소형저18항혈액생리생화지표통계결과현시,홍세포수(RBC)차이(P<0.01)유통계학의의,기타지표차이균무통계학의의.결론 근거선립체DNA공제구서렬감기변이,서장소형저분위량개류군,량개군체지간홍세포수존재명현차이.
Objective To analyze the mitochondrial DNA (mtDNA) D-loop region sequence variation in Tibet Mini-Pigs in relation to the blood parameters and provide the molecular genetic basis for developing new species of laboratory animals.Methods The genomic DNA was extracted from the whole blood samples of 59 Tibet mini-pigs to amplifying the mtDNA D-loop for sequence analysis. Nine physiological and nine biochemical blood parameters of Tibet mini-pigs were measured .Results Based on the variation of the tandem repeat motif, the mtDNA D-loop region of Tibet mini-pigs was classified into two types, namely type A and B with the percentage of 57.6% and 42.4%, respectively, roughly matching the 3 transform sites (305, 500, 691) at the 5' end. In the 18 blood parameters, only red blood cell count showed significant differences between types A and (P<0.01). Conclusion Based on the sequence variation of the mtDNA D-loop region, Tibet mini-pigs can be divided into two types that show a significant difference in red blood cell count.