CAJ | 학술논문

为探讨外源性核苷酸对啮齿类大鼠亲代与子代生长发育的影响,选用SPF级断乳SD大鼠(F0),将大鼠随机分为对照组和5个外源核苷酸剂量组(核苷酸含量分别为0.01%、0.04%、0.16%、0.64%和1.28%),每组雌雄各30只.F0喂含受试物饲料90d,之后雌、雄1:1交配,所产仔鼠为F1.F1断乳后再随机分成2个亚组(F1A和F1B),每亚组10窝动物.F1的A亚组给与对照饲料,F1的B亚组继续给与同其亲本(F0)的饲料,喂养至断乳后90d.测定F0母鼠乳汁中核苷酸含量.F0、F1断乳后喂养期间称量体重,计算食物利用率,断乳后90d进行血液指标检测.结果显示,外源核苷酸能够促进亲代与子代大鼠生长发育、提高抗断乳应激的能力.
위탐토외원성핵감산대교치류대서친대여자대생장발육적영향,선용SPF급단유SD대서(F0),장대서수궤분위대조조화5개외원핵감산제량조(핵감산함량분별위0.01%、0.04%、0.16%、0.64%화1.28%),매조자웅각30지.F0위함수시물사료90d,지후자、웅1:1교배,소산자서위F1.F1단유후재수궤분성2개아조(F1A화F1B),매아조10와동물.F1적A아조급여대조사료,F1적B아조계속급여동기친본(F0)적사료,위양지단유후90d.측정F0모서유즙중핵감산함량.F0、F1단유후위양기간칭량체중,계산식물이용솔,단유후90d진행혈액지표검측.결과현시,외원핵감산능구촉진친대여자대대서생장발육、제고항단유응격적능력.
To investigate the effect of exogenous nueleotides on the development of parental and filial rats, weanling SD rats (F0) were randomly divided into the control group and 5 dose groups according to different doses of exogenous nucleotides (0.01%o 0.04%, 0.16%, 0.64% and 1.28%) with 30 rats in each. F0 rats were fed with exogenous nucleotides for 90 days and then male and female rats were mated in 1:1 pattern. The offspring of F0 is called F1. After weaned, F1 rats were randomly divided into two groups (F1A and F1B) with ten broods in each group. F1A rats were fed the same as the control group and F1B rats were fed with exogenous nucleotides as the parental generations for 90 days. The doses of nucleotides in milk of F0 were measured and after F0 and F1 rats were weaned, the body weight of each rat was measured once a week, and then food utilization was calculated. In the end, the blood parameters were measured. The results show that exogenous nucleotides could promote the physique development and improve the anti-weaned stress ability in parental and filial rats.