CAJ | 학술논문

目的:研究莲子壳中的黄酮类化学成分及抗氧化活性.方法:莲子壳的60%乙醇提取物经过大孔树脂初步分离之后,采用Toyopearl HW-40S凝胶柱进一步分离得到了6个组分(LF-F_1～LF-F_6),再采用高效液相以及液质联用系统对其中的组分2(LF-F_2)组成及结构进行分析,并采用化学发光法等方法研究了LF-F_2的抗氧化活性.结果:莲子壳黄酮粗提物中总黄酮为94.1%;LF-F_2中黄酮甙元是由槲皮素、杨梅黄酮、山奈酚、异鼠李素等组成的.LF-F_2对超氧阴离子自由基、羟自由基以及对双氧水的清除作用的IC_(50)分别为420、40、0.6μg/ml.结论:从莲子壳中鉴定出LF-F_2中有芦丁存在.LF-F_2具有很好的抗氧化效果,并存在剂量效应关系.
목적:연구련자각중적황동류화학성분급항양화활성.방법:련자각적60%을순제취물경과대공수지초보분리지후,채용Toyopearl HW-40S응효주진일보분리득도료6개조분(LF-F_1～LF-F_6),재채용고효액상이급액질련용계통대기중적조분2(LF-F_2)조성급결구진행분석,병채용화학발광법등방법연구료LF-F_2적항양화활성.결과:련자각황동조제물중총황동위94.1%;LF-F_2중황동대원시유곡피소、양매황동、산내분、이서리소등조성적.LF-F_2대초양음리자자유기、간자유기이급대쌍양수적청제작용적IC_(50)분별위420、40、0.6μg/ml.결론:종련자각중감정출LF-F_2중유호정존재.LF-F_2구유흔호적항양화효과,병존재제량효응관계.
This study was aiming to study the chemical composition of flavonoids of ethanolic extract of Epicarp of Nelumbo nucifera Gaertn as well as its antioxidant activity. The ethaonolic extract of lotus Epicarp was isolated using macroporous resin and Toyopearl HW-40S gel column to acquired 6 compounds (LF-F_1～LF-F6). The composition and structure of LF-F2 was analyzed by HPLC and LC/MS and the antioxidant activity was measured by chemiluminescence. Results showed that total flavonoids accounts for 94.1% of the extracts (60% ethanol) of lotus Epicarp. Flavonols of LF-F2 comprised quercetin, myricetin,kaempferol, isorhamnetin and so oa IC_(50) for LF-F_2 to superoxide anion radical, hydroxyl radical and hydrogen peroxide were 420,40, 0.6μg/ml, respectively. The existence of rutin in LF-F_2 was identified for the first time. Fraction LF-F_2 possessed good antioxidant activity in a dose dependent manner.