CAJ | 학술논문

黄伞菌丝体烘干后经热水提取,乙醇沉淀得粗多糖.粗多糖经Sevag法除蛋白后DEAE-纤维素柱层析,分离得到两种多糖组分PAMP Ⅰ和PAMP Ⅱ.红外光谱分析表明PAMP Ⅰ和PAMP Ⅱ均为含有葡萄糖醛酸的β-D-吡喃葡聚糖.采用化学比色法测定黄伞菌丝体多糖在体外的总抗氧化能力、抑制活性超氧阴离子自由基的能力、清除羟自由基的能力及对小鼠肝组织中脂质过氧化产物丙二醛(MDA)含量的的影响.结果表明:一定浓度范围内不同纯度的黄伞菌丝体多糖均有总抗氧化能力,能清除O_2~-·和·OH及抑制MDA的生成,且呈现一定剂量关系.表明黄伞菌丝体多糖在一定浓度范围内具有抗氧化作用.
황산균사체홍간후경열수제취,을순침정득조다당.조다당경Sevag법제단백후DEAE-섬유소주층석,분리득도량충다당조분PAMP Ⅰ화PAMP Ⅱ.홍외광보분석표명PAMP Ⅰ화PAMP Ⅱ균위함유포도당철산적β-D-필남포취당.채용화학비색법측정황산균사체다당재체외적총항양화능력、억제활성초양음리자자유기적능력、청제간자유기적능력급대소서간조직중지질과양화산물병이철(MDA)함량적적영향.결과표명:일정농도범위내불동순도적황산균사체다당균유총항양화능력,능청제O_2~-·화·OH급억제MDA적생성,차정현일정제량관계.표명황산균사체다당재일정농도범위내구유항양화작용.
Crude polysaccharides were obtained from Pholiota adiposa Mycelium through hot-water extraction and ethanol precipitation. Crude polysaccharides after removing protein through Sevage method were purified using DEAE-cellulose column chromatography to obtain two polysaccharide ingredients, designated as PAMP-Ⅰ and PAMP-Ⅱ Glucuronic acid β-D-pyran-glucan was identified in both purified polysacchride components through IR spectrum analysis. Total anti-oxidant capability,scavenging capability to superoxide anion and hydroxyl free radicals, and effect of polysaccharides from Pholiota adiposa Mycelium on MDA content were evaluated using colorimetry. Results indicated that polysaccharides from Pholiota adiposa Mycelium had anti-oxidant capability such as scavenging free radicals and suppressing MDA generation in a dose-dependent mode.