CAJ | 학술논문

用与天然磷脂结构和相对分子质量类似的磷脂月E{碱聚乳酸(Phosphorylcholine-containing poly(L-lactide),PLLA.pc)$sJ备自纽装胶束,采朋透射电镜和激光共聚焦显微镜进行观察.研究结果表明:胶束为外壳亲水内核疏水的实心球:表面张力法测得临界胶束质量浓度(Critical micelle concentration,CMC)~23 mg/L,显著低于天然磷脂胆碱质鼍浓度;当氯化钠质量分数低于0.9%时,CMC无明显变化,表明胶柬在生理盐水中较稳定:胶束被稀释时,动态光散射测得胶束粒径和粒径分布均逐渐变小,表明体系是动态体系;在37℃恒温条件下放置15 h,胶束数量、粒径和分布均变小,表明胶束可随时间降解;PLLA-PC胶束生物相容性好,可降解,可望成为新型的控释药物载体.
용여천연린지결구화상대분자질량유사적린지월E{감취유산(Phosphorylcholine-containing poly(L-lactide),PLLA.pc)$sJ비자뉴장효속,채붕투사전경화격광공취초현미경진행관찰.연구결과표명:효속위외각친수내핵소수적실심구:표면장역법측득림계효속질량농도(Critical micelle concentration,CMC)~23 mg/L,현저저우천연린지담감질타농도;당록화납질량분수저우0.9%시,CMC무명현변화,표명효간재생리염수중교은정:효속피희석시,동태광산사측득효속립경화립경분포균축점변소,표명체계시동태체계;재37℃항온조건하방치15 h,효속수량、립경화분포균변소,표명효속가수시간강해;PLLA-PC효속생물상용성호,가강해,가망성위신형적공석약물재체.
Self-assembled micelles were prepared from phosphorylcholine-containing poly (L-lactide)(PLLA-PC) with similar molecular structure and molecular mass to natural phospholipids.The global structure with hydrophilic shell and hydrophobic core is confirmed by transmission electron microscope (TEM) and confocal laser scanning microscope (CLSM).The results show that critical micelle concentration (CMC) is detected to be 23 mg/L with surface tension method, which is much lower than that of natural phospholipids.It remains constant while NaCl concentration varies within 0.9% (mass fraction), indicating the stability of micelles in physiological saline.The results of dynamic light scattering (DLS) show that the diameter and distribution of micelles decrease gradually with dilution, suggesting a dynamic system.Moreover, the number, particle size and distribution of micelles diminish after 15 d at 37 'C, indicating that the micelles can degrade over time.With good biocompatibility and biodegradability PLLA-PC micelle might be a novel alternative for controlled drug release.