CAJ | 학술논문

2006年4月,从流沙港马氏珠母贝养殖群体中挑选11个纯黄壳色个体为繁殖群体建立了马氏珠母贝(Pinctadamartensii)黄壳色选系F_1(SG_1).同时,随机选取养殖群体中50个个体作为繁殖群体建立了对照组(CG).2007年10月,从2个组分别选取相同规格的个体,比较了生长适温(15～30℃)下2个组的消化酶活力.结果表明:(1)马氏珠母贝肝胰脏中具有淀粉酶、纤维素酶和蛋白酶活力,在15～30℃条件下,三者活力由高到低依次为淀粉酶、纤维素酶、蛋白酶.(2)在15～30℃范围内,马氏珠母贝淀粉酶活力随温度升高先上升再下降,在温度25℃时达到最大值;SG_1组和CC组淀粉酶活力变化范同分别为3.51～5.09μg·min~(-1)·mg~(-1)和2.53～4.04μg·min~(-1)·mg~(-1);各反应温度下,SG_1组淀粉酶活力均高于CG组,在15℃和20℃时,二者差异显著(P<0.05).(3)在15～30℃范嗣内,马氏珠母贝纤维素酶活力随温度上升而上升,在温度30℃时达到最大值;SG_1组和CG组纤维素酶活力变化范围为(2.44～3.22)μg·min~(-1)·mg~(-1)和(2.07～3.12)μg·min~(-1)·mg~(-1);各实验温度下,SG_1组纤维素酶活力均高于CG组,但差异均不显著(P>0.05).(4)在15～30℃范围内,马氏珠母贝蛋白酶活力随温度上升而升高,在温度30℃时达到最大值;SG_1组和CG组蛋白酶活力变化范围为(0.075～0.296)μg·min~(-1)·mg~(-1)和(0.067～0.455)μg·min~(-1)·mg~(-1).在15℃时,SG_1组蛋白酶活力大于对照组,差异不显著(P>0.05);在20℃、25℃、30℃时,CG组蛋白酶活力显著大于SG_1组(P<0.05).本研究结果表明,经过一代壳色选育后黄壳色选系与对照组的消化生理指标存在明显差异,为马氏珠母贝的黄壳色系进一步选育提供依据.
2006년4월,종류사항마씨주모패양식군체중도선11개순황각색개체위번식군체건립료마씨주모패(Pinctadamartensii)황각색선계F_1(SG_1).동시,수궤선취양식군체중50개개체작위번식군체건립료대조조(CG).2007년10월,종2개조분별선취상동규격적개체,비교료생장괄온(15～30℃)하2개조적소화매활력.결과표명:(1)마씨주모패간이장중구유정분매、섬유소매화단백매활력,재15～30℃조건하,삼자활력유고도저의차위정분매、섬유소매、단백매.(2)재15～30℃범위내,마씨주모패정분매활력수온도승고선상승재하강,재온도25℃시체도최대치;SG_1조화CC조정분매활력변화범동분별위3.51～5.09μg·min~(-1)·mg~(-1)화2.53～4.04μg·min~(-1)·mg~(-1);각반응온도하,SG_1조정분매활력균고우CG조,재15℃화20℃시,이자차이현저(P<0.05).(3)재15～30℃범사내,마씨주모패섬유소매활력수온도상승이상승,재온도30℃시체도최대치;SG_1조화CG조섬유소매활력변화범위위(2.44～3.22)μg·min~(-1)·mg~(-1)화(2.07～3.12)μg·min~(-1)·mg~(-1);각실험온도하,SG_1조섬유소매활력균고우CG조,단차이균불현저(P>0.05).(4)재15～30℃범위내,마씨주모패단백매활력수온도상승이승고,재온도30℃시체도최대치;SG_1조화CG조단백매활력변화범위위(0.075～0.296)μg·min~(-1)·mg~(-1)화(0.067～0.455)μg·min~(-1)·mg~(-1).재15℃시,SG_1조단백매활력대우대조조,차이불현저(P>0.05);재20℃、25℃、30℃시,CG조단백매활력현저대우SG_1조(P<0.05).본연구결과표명,경과일대각색선육후황각색선계여대조조적소화생리지표존재명현차이,위마씨주모패적황각색계진일보선육제공의거.
The first-generation selected group (SG_1)was established by eleven breeders with yellow shell color selected from Liusha Bay stock of pearl oyster Pinctada martensii in April 2006. A control group (CG) of fifty mature individuals was also obtained by randomly selecting from the same stock as breeders. In October 2007, the individuals with uniform sizes were selected from SG_1 and CG groups, and then amylase, cellulose and protease activities between the two groups were compared at 15 ℃,20 ℃,25 ℃ and 30℃. The results were as follows: (1)The activity of amylase was the highest, followed by cellulose and protease activities at different temperature. (2)At the temperature range of 15 ℃ to 30 ℃, the amylase activity of the two groups increased with the increasing temperature, reaching peak value at 25 ℃, and then decreased beyond 25 ℃. The amylase activities of SG_1 and CG varied from 3.51 to 5.09 μg·min~(-1)·mg~(-1) and from 2.53 to 4.04 μg·min~(-1)·mg~(-1), respectively. At 15 ℃,20 ℃,25 ℃ and 30 ℃, the amylase activity of SG_1 was higher than that of CG, but with significant differences observed only at 15 ℃ and 20 ℃ (P<0.05). (3) In the experiment temperatures range, the cellulose activity were positively correlated to the ambient temperature, with values of 2.44-3.22μg·min~(-1)·mg~(-1) in SG_1 and 2.07-3.12 μg·min~(-1)·mg~(-1) in CG, respectively. No significant difference was observed in the two groups (P>0.05). (4) At 15-30 ℃, the protease activity showed a positive correlation to the ambient temperature. The protease activities of SG_1 were much higher than that of CG at 15 ℃, with insignificant differences observed (P>0.05). On the contrary, the CG exhibited higher protease activities than SG_1 at 20 ℃,25 ℃ and 30 ℃. The results from the present study indicate evident differences in physiological indices between SG_1 and CG, which will provide much more useful information for further breeding.