CAJ | 학술논문

探讨当归内酯(ASDL)对免疫抑制小鼠免疫功能的重构作用.通过小鼠腹腔注射环磷酰胺建立免疫抑制动物模型.采用免疫器官重量法和小鼠腹腔巨噬细胞吞噬鸡红细胞实验检测了ASDL对非特异性免疫功能的影响;用血清溶血素分光光度法检测了对体液免疫功能的作用;用MTT法进行了致分裂原诱导的小鼠脾淋巴细胞的增值反应实验,再用乳酸脱氢酶法测定了NK和CTL细胞活性,从而确定ASDL对小鼠细胞免疫功能的影响.结果表明:ASDL能够对免疫低下小鼠的非特性和特异性免疫功能起到一定的重构作用.但是这种效果并不是剂量依赖性的,20 mg/kg这个剂量的效果明显好于5和80 mg/kg这两个剂量.上述结果表明ASDL能够显著提高免疫低下小鼠的免疫功能.
탐토당귀내지(ASDL)대면역억제소서면역공능적중구작용.통과소서복강주사배린선알건립면역억제동물모형.채용면역기관중량법화소서복강거서세포탄서계홍세포실험검측료ASDL대비특이성면역공능적영향;용혈청용혈소분광광도법검측료대체액면역공능적작용;용MTT법진행료치분렬원유도적소서비림파세포적증치반응실험,재용유산탈경매법측정료NK화CTL세포활성,종이학정ASDL대소서세포면역공능적영향.결과표명:ASDL능구대면역저하소서적비특성화특이성면역공능기도일정적중구작용.단시저충효과병불시제량의뢰성적,20 mg/kg저개제량적효과명현호우5화80 mg/kg저량개제량.상술결과표명ASDL능구현저제고면역저하소서적면역공능.
This paper studied the effects of lactone extracts from Angelica sinensis (Oliv.) Diels (ASDL) on immunity functions in immunocompromised mice by different methods. The nonspecific immune function was measured by thymus, spleen index and macrophage phagocytosis. Determination of hemolysin concentration in serum by Quantitative hemolysis of sheep red blood cells assay revealed the humoral function. In addition, the proliferative response of lymphocyte to concanavalin A nd lipopolysaccharides,the activity of cytotoxic T lymphocyte and nature killer cells activity were studied to demonstrate the effects of ASDL on cellular immunogical functions. These effects were not dose-dependent in a linear fashion. A total of 20 mg/kg dose is more effective than 5 and 80mg/kg doses. In summary,we conclude that the oral treatment of immunocompromised mice with ASDL led to partial reconstitution of the nonspecific, humoral and cellular immune response.