CAJ | 학술논문

目的探讨高强度超声对环氧化酶-2( COX-2) mRNA的表达及其影响乳腺癌MDA-MB-231细胞凋亡的作用机制.方法用高强度超声(50 w/cm2)干预体外培养的人乳腺癌MDA-MB-231细胞.RT-PCR检测干预前后MDA-MB-231的COX-2 mRNA的相对水平,透射电镜观察细胞超微结构.结果随着高强度超声干预时间的增加,COX-2 mRNA相对水平逐渐下调,电镜下可见明显的凋亡小体.干预时间增加到30 s,部分细胞出现死亡的形态学改变.结论高强度超声干预在一定作用时间内促进乳腺癌MDA-MB-231细胞COX-2依赖性的细胞凋亡.
목적 탐토고강도초성대배양화매-2( COX-2) mRNA적표체급기영향유선암MDA-MB-231세포조망적작용궤제.방법 용고강도초성(50 w/cm2)간예체외배양적인유선암MDA-MB-231세포.RT-PCR검측간예전후MDA-MB-231적COX-2 mRNA적상대수평,투사전경관찰세포초미결구.결과 수착고강도초성간예시간적증가,COX-2 mRNA상대수평축점하조,전경하가견명현적조망소체.간예시간증가도30 s,부분세포출현사망적형태학개변.결론 고강도초성간예재일정작용시간내촉진유선암MDA-MB-231세포COX-2의뢰성적세포조망.
Objective To study the mechanism of high intensity ultrasound (HIU) on COX-2 mRNA expression and apoptosis in breast cancer cell line MDA-MB-231 cells.Methods Human breast cancer cells MDA-MB-231 in vitro were exposed in HIU (50 w/cm2).RT-PCR was performed to detect the level of COX-2 mRNA before and after exposure.The ultrastructural changes in apoptotic cells were examined by transmission electron microscopy.Results The relative level of COX-2 mRNA decreased gradually along with the increase of exposure time and apoptotic bodies in MDA-MB-231 cells considerably increased under electron microscope.When exposure time was increased to 30 seconds,a few cells died.Conclusion HIU promotes apoptosis of breast cancer MDA-MB-231 cells in a COX-2 mRNA depended way.