中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2012年
6期
750-752,755
,共4页
谢谦%张帆%江智茂%戴亚丽%张宁波
謝謙%張帆%江智茂%戴亞麗%張寧波
사겸%장범%강지무%대아려%장저파
受体,自身分泌活动因子/代谢%甲状腺肿瘤/代谢/病理学%癌,乳头状/代谢/病理学
受體,自身分泌活動因子/代謝%甲狀腺腫瘤/代謝/病理學%癌,乳頭狀/代謝/病理學
수체,자신분비활동인자/대사%갑상선종류/대사/병이학%암,유두상/대사/병이학
Receptors,autocrine motility factor/metabolism%Thyroid neoplasms/metabolism/pathology%Carcinoma,papillary/metabolism/pathology
目的 探讨自分泌运动因子受体(autocrine motility factor receptor,AMFR)在甲状腺乳头状癌中的表达情况,以及其与临床的关系.方法 采用实时荧光定量PCR和免疫组化的方法对甲状腺乳头状癌进行研究.结果 甲状腺乳头状癌和甲状腺正常组织在AMFR的mRNA和蛋白水平差异均有统计学意义(6.296±1.568 vs7.913±2.351,t=3.681,P=0.001;63.1％vs 34.5％,x2 =13.722,P＜0.001).免疫组化分析显示甲状腺乳头状癌中AMFR蛋白的水平与肿瘤大小(x2=5.209,P＜0.05)和淋巴结转移有关(x2=4.32,P＜0.05),而与性别(x2=0.739,P=0.39)、年龄无关(x2 =0.064,P=0.81).结论 AMFR在甲状腺乳头状癌中表达升高,其可能成为甲状腺乳头状癌治疗中的一个新靶点和预后的新指标.
目的 探討自分泌運動因子受體(autocrine motility factor receptor,AMFR)在甲狀腺乳頭狀癌中的錶達情況,以及其與臨床的關繫.方法 採用實時熒光定量PCR和免疫組化的方法對甲狀腺乳頭狀癌進行研究.結果 甲狀腺乳頭狀癌和甲狀腺正常組織在AMFR的mRNA和蛋白水平差異均有統計學意義(6.296±1.568 vs7.913±2.351,t=3.681,P=0.001;63.1％vs 34.5％,x2 =13.722,P＜0.001).免疫組化分析顯示甲狀腺乳頭狀癌中AMFR蛋白的水平與腫瘤大小(x2=5.209,P＜0.05)和淋巴結轉移有關(x2=4.32,P＜0.05),而與性彆(x2=0.739,P=0.39)、年齡無關(x2 =0.064,P=0.81).結論 AMFR在甲狀腺乳頭狀癌中錶達升高,其可能成為甲狀腺乳頭狀癌治療中的一箇新靶點和預後的新指標.
목적 탐토자분비운동인자수체(autocrine motility factor receptor,AMFR)재갑상선유두상암중적표체정황,이급기여림상적관계.방법 채용실시형광정량PCR화면역조화적방법대갑상선유두상암진행연구.결과 갑상선유두상암화갑상선정상조직재AMFR적mRNA화단백수평차이균유통계학의의(6.296±1.568 vs7.913±2.351,t=3.681,P=0.001;63.1％vs 34.5％,x2 =13.722,P＜0.001).면역조화분석현시갑상선유두상암중AMFR단백적수평여종류대소(x2=5.209,P＜0.05)화림파결전이유관(x2=4.32,P＜0.05),이여성별(x2=0.739,P=0.39)、년령무관(x2 =0.064,P=0.81).결론 AMFR재갑상선유두상암중표체승고,기가능성위갑상선유두상암치료중적일개신파점화예후적신지표.
Objective To investigate the expression of autocrine motility factor receptor (AMFR)in papillary thyroid carcinoma and its relationship with clinical characteristics of this disease.Methods Real - time quantitative PCR and immunohistochemical methods were used to analyze the expression of AMFR in papillary thyroid carcinomas.Results The significant differences in AMFR expression between papillary thyroid carcinoma and normal thyroid tissues were found in the levels of mRNA (6.296 ± 1.568 vs 7.913 ± 2.351,t=3.681,P=0.001 ) and protein ( 63.1％ vs 34.5 ％,x2=13.722,P ＜ 0.001 ),respectively.Immunohistochemistry analyses showed that the protein expression of AMFR in papillary thyroid carcinomas were significantly correlated with tumour size ( x2=5.209,P ＜ 0.05 ) and lymph node metastasis ( x2=4.32,P ＜ 0.05 ),and it was affected by the factors age ( x2=0.739,P=0.39 ) and gender ( x2=0.064,P=0.81 ).Conclusions The increased AMFR in papillary thyroid carcinoma would be a new target for cancer therapy and a new marker for prognosis.
