中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2011年
7期
633-635
,共3页
癫痫%Orexin-A%学习和记忆
癲癇%Orexin-A%學習和記憶
전간%Orexin-A%학습화기억
Epilepsy%Orexin-A%Learning and memory
目的 探讨Orexin-A对戊四氮(PTZ)慢性点燃癫痫大鼠学习记忆功能的影响.方法 健康成年雄性Wistar大鼠随机分为对照组(生理盐水)和戊四氮组.点燃成功后每组分别脑室内注射10μlorexin-A(1.4 nmol/μl)或等量生理盐水,利用Morris水迷宫实验进行学习记忆能力检测.结果 致痫组戊四氮点燃癫痫的成功率达80%.与非致病组比较,慢性点燃大鼠搜寻平台的潜伏期明显延长(F=200.956,P<0.01);大鼠在平台所在象限的游泳时间和120 s内穿越平台区域的次数显著减少(P<0.01).脑室内注射orexin-A后,航行定位实验中致痫组大鼠逃避潜伏期明显缩短(P<0.01),非致痫组大鼠的逃避潜伏期也明显缩短(P<0.05).在空间搜寻实验中,orexin-A能明显增加穿越平台象限的次数[(10.83±1.84),(4.67±3.34)]和平台所在象限的游泳时间[(39.73±2.03)s,(33.76±2.96)s],尤其是对致痫组大鼠的怍用更明显(P<0.01),搜寻站台的效率明显提高.结论 Orexin-A能改善慢性点燃癫痫大鼠的空间学习记忆能力.
目的 探討Orexin-A對戊四氮(PTZ)慢性點燃癲癇大鼠學習記憶功能的影響.方法 健康成年雄性Wistar大鼠隨機分為對照組(生理鹽水)和戊四氮組.點燃成功後每組分彆腦室內註射10μlorexin-A(1.4 nmol/μl)或等量生理鹽水,利用Morris水迷宮實驗進行學習記憶能力檢測.結果 緻癇組戊四氮點燃癲癇的成功率達80%.與非緻病組比較,慢性點燃大鼠搜尋平檯的潛伏期明顯延長(F=200.956,P<0.01);大鼠在平檯所在象限的遊泳時間和120 s內穿越平檯區域的次數顯著減少(P<0.01).腦室內註射orexin-A後,航行定位實驗中緻癇組大鼠逃避潛伏期明顯縮短(P<0.01),非緻癇組大鼠的逃避潛伏期也明顯縮短(P<0.05).在空間搜尋實驗中,orexin-A能明顯增加穿越平檯象限的次數[(10.83±1.84),(4.67±3.34)]和平檯所在象限的遊泳時間[(39.73±2.03)s,(33.76±2.96)s],尤其是對緻癇組大鼠的怍用更明顯(P<0.01),搜尋站檯的效率明顯提高.結論 Orexin-A能改善慢性點燃癲癇大鼠的空間學習記憶能力.
목적 탐토Orexin-A대무사담(PTZ)만성점연전간대서학습기억공능적영향.방법 건강성년웅성Wistar대서수궤분위대조조(생리염수)화무사담조.점연성공후매조분별뇌실내주사10μlorexin-A(1.4 nmol/μl)혹등량생리염수,이용Morris수미궁실험진행학습기억능력검측.결과 치간조무사담점연전간적성공솔체80%.여비치병조비교,만성점연대서수심평태적잠복기명현연장(F=200.956,P<0.01);대서재평태소재상한적유영시간화120 s내천월평태구역적차수현저감소(P<0.01).뇌실내주사orexin-A후,항행정위실험중치간조대서도피잠복기명현축단(P<0.01),비치간조대서적도피잠복기야명현축단(P<0.05).재공간수심실험중,orexin-A능명현증가천월평태상한적차수[(10.83±1.84),(4.67±3.34)]화평태소재상한적유영시간[(39.73±2.03)s,(33.76±2.96)s],우기시대치간조대서적작용경명현(P<0.01),수심참태적효솔명현제고.결론 Orexin-A능개선만성점연전간대서적공간학습기억능력.
Objective To investigate the effects of orexin-A on learning and memory of pentyleneterazol (PTZ)-kindled epileptic rats.Methods Adult Wistar rats were randomly divided into control group(normal saline,NS)and PTZ group.The PTZ-kindled rats were randomly divided into,orexin-A group and NS group administrated by intracerebroventricular(i.c.v.)injection of 10μl orexin-A(1.4 nmol/μl)or 10μl NS.Using Morris water msze experiment,the ability of learning and memory was measured in all rats.Results Eighty percent of rats in PIZ group were kindled successfully after intraperitoneal injection of 35mg/kg PTZ every day for 30 days.Compared to control group,the escape latency in the PTZ-kindled rats was significantly increased in place navigation test(PNT)(F=200.956,P<0.01),whereas a remarkable reduction of time spent in the target quadrant and number of pool circlings in 120 seconds Was observed during probe trials.Following injection of orexin-A,the latency of escape platform was significantly declined in both PTZ-kindled((39.73±2.03)8,(33.76±2.96)s)and NS rats,increased the number of crossing the platform(10.83±1.80)vs(4.67±3.34).In addition,the treatment with orexin-A markedly increased swim velocity and number of pool circlings in beth groups(P<0.01),particularly to the PTZ-kindled rats.Conclusion Spatial learning and memory in the PIZ-kindled rats can be improved by treatment with orexin-A.
