中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2012年
5期
389-391
,共3页
于雪%杨坤%刘皓%凌孝征%李艳芸
于雪%楊坤%劉皓%凌孝徵%李豔蕓
우설%양곤%류호%릉효정%리염예
抗抑郁剂%应激%学习记忆%星形胶质源蛋白
抗抑鬱劑%應激%學習記憶%星形膠質源蛋白
항억욱제%응격%학습기억%성형효질원단백
Antidepressant%Stress%Learning and memory%Astroglia-derived protein S100B
目的 探讨氟西汀对应激抑郁模型大鼠空间学习记忆和血清S100B水平的影响.方法 将成年雄性SD大鼠按照随机数字表分为6组:对照组(A)、抑郁模型组(B)、抑郁模型+给药1天组(C)、抑郁模型+给药1周组(D)、抑郁模型+给药2周组(E)和抑郁模型+给药4周组(F),每组动物10只,抑郁模型为强迫大鼠游泳.氟西汀的剂量为10 mg/kg,给药方式为灌胃.应用Morris水迷宫测试大鼠的空间学习记忆情况.采用ELISA检测各组大鼠血清S100B水平.结果 在Morris水迷宫定位航行实验中,B组大鼠平均逃避潜伏期显著大于A组(P＜0.05)；E组、F组与B组比较,其平均逃避潜伏期缩短,差异有统计学意义(P＜0.05)；各组空间探索中,B组大鼠在第一象限的停留时间显著较A组长(P＜0.05)；D、E、F大鼠在目标象限的时间(第三象限)明显长于在其它3个象限的停留时间(F=5.162,P＜0.01).E组和F组血清S100B水平[(0.91±0.23) ng/ml,(0.85±0.21) ng/ml]显著低于B组[(1.26±0.61) ng/ml,P＜0.05],但与对照组[(0.92 ±0.30ng/ml)]比较,差异无统计学意义(P＞0.05),抑郁模型状态水迷宫行为学指标(平均逃避潜伏期、偏离角、过台次数及平台象限游泳时间)与血清S100B水平无显著性相关关系(相关系数分别为0.107,0.068,-0.174,-0.09).结论 氟西汀长期用药明显改善抑郁模型大鼠空间学习记忆的损害,逆转抑郁模型大鼠升高的血清S100B水平.
目的 探討氟西汀對應激抑鬱模型大鼠空間學習記憶和血清S100B水平的影響.方法 將成年雄性SD大鼠按照隨機數字錶分為6組:對照組(A)、抑鬱模型組(B)、抑鬱模型+給藥1天組(C)、抑鬱模型+給藥1週組(D)、抑鬱模型+給藥2週組(E)和抑鬱模型+給藥4週組(F),每組動物10隻,抑鬱模型為彊迫大鼠遊泳.氟西汀的劑量為10 mg/kg,給藥方式為灌胃.應用Morris水迷宮測試大鼠的空間學習記憶情況.採用ELISA檢測各組大鼠血清S100B水平.結果 在Morris水迷宮定位航行實驗中,B組大鼠平均逃避潛伏期顯著大于A組(P＜0.05)；E組、F組與B組比較,其平均逃避潛伏期縮短,差異有統計學意義(P＜0.05)；各組空間探索中,B組大鼠在第一象限的停留時間顯著較A組長(P＜0.05)；D、E、F大鼠在目標象限的時間(第三象限)明顯長于在其它3箇象限的停留時間(F=5.162,P＜0.01).E組和F組血清S100B水平[(0.91±0.23) ng/ml,(0.85±0.21) ng/ml]顯著低于B組[(1.26±0.61) ng/ml,P＜0.05],但與對照組[(0.92 ±0.30ng/ml)]比較,差異無統計學意義(P＞0.05),抑鬱模型狀態水迷宮行為學指標(平均逃避潛伏期、偏離角、過檯次數及平檯象限遊泳時間)與血清S100B水平無顯著性相關關繫(相關繫數分彆為0.107,0.068,-0.174,-0.09).結論 氟西汀長期用藥明顯改善抑鬱模型大鼠空間學習記憶的損害,逆轉抑鬱模型大鼠升高的血清S100B水平.
목적 탐토불서정대응격억욱모형대서공간학습기억화혈청S100B수평적영향.방법 장성년웅성SD대서안조수궤수자표분위6조:대조조(A)、억욱모형조(B)、억욱모형+급약1천조(C)、억욱모형+급약1주조(D)、억욱모형+급약2주조(E)화억욱모형+급약4주조(F),매조동물10지,억욱모형위강박대서유영.불서정적제량위10 mg/kg,급약방식위관위.응용Morris수미궁측시대서적공간학습기억정황.채용ELISA검측각조대서혈청S100B수평.결과 재Morris수미궁정위항행실험중,B조대서평균도피잠복기현저대우A조(P＜0.05)；E조、F조여B조비교,기평균도피잠복기축단,차이유통계학의의(P＜0.05)；각조공간탐색중,B조대서재제일상한적정류시간현저교A조장(P＜0.05)；D、E、F대서재목표상한적시간(제삼상한)명현장우재기타3개상한적정류시간(F=5.162,P＜0.01).E조화F조혈청S100B수평[(0.91±0.23) ng/ml,(0.85±0.21) ng/ml]현저저우B조[(1.26±0.61) ng/ml,P＜0.05],단여대조조[(0.92 ±0.30ng/ml)]비교,차이무통계학의의(P＞0.05),억욱모형상태수미궁행위학지표(평균도피잠복기、편리각、과태차수급평태상한유영시간)여혈청S100B수평무현저성상관관계(상관계수분별위0.107,0.068,-0.174,-0.09).결론 불서정장기용약명현개선억욱모형대서공간학습기억적손해,역전억욱모형대서승고적혈청S100B수평.
Objective To explore the effects of fluoxetine on special learning and memory and serum S100B level in depressed model rats.Methods Adult male SD rats were divided into six groups randomly according random digits table:control group ( A ),depressed model group ( B ),group of depressed model treated with single dose of fluoxetine for one day ( C ),group of depressed model treated with fluoxetine for one week (D),group of depressed model treated with fluoxetine for two weeks (E) and group of depressed model treated with fluoxetine for four weeks (F),ten rats in each group.Except control group,others were subjected to forced-swimming for four weeks,15 min a day.Fluoxetine (10 mg/kg) was given intragastric administration to group C-F before swimming everyday.Morris water maze ( MWM ) was used to measure the spatial learning and memory of rats.ELISA was used to determine the level of serum S100B.Results In the hiding platform test of MWM,there was significant longer of escape latency (EL) in B group than that in A group(P ＜ 0.05 ).And the EL in all groups treated with fluoxetine became shorter with the prolonging of treatment.In the probe test,there were significant longer time in target quadrant in D,E,F than in other quadrant (F =5.162,P ＜ 0.01 ).The levels of serum S100B were lower in E,F groups ( E group ( 0.91 ± 0.23 ) ng/ml,F group ( 0.85 ± 0.21 ) ng/ml) than that in B group (( 1.26 ±0.61 )ng/ml,P＜0.05).Conclusion Chronic administration of fluoxetine could improve the impairment of spatial learning and memory and reverse the increase of S100B level in serum of depressed model rats.
