中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2010年
4期
375-378
,共4页
张瑛琦%郭敏%乔国芬%成志锋
張瑛琦%郭敏%喬國芬%成誌鋒
장영기%곽민%교국분%성지봉
甲状腺功能亢进症%腹泻%阿是止泻胶囊%杯状细胞
甲狀腺功能亢進癥%腹瀉%阿是止瀉膠囊%杯狀細胞
갑상선공능항진증%복사%아시지사효낭%배상세포
Hyperthyroidism%Dysentery%Asi-antidiarrheal capsule%Goblet cells
目的 探讨阿是止泻胶囊对甲状腺功能亢进性腹泻(简称甲亢性腹泻)大鼠胃肠道杯状细胞的影响.方法 8周龄的雄性SD大鼠120只,按体质量随机分为对照组(10只)和甲亢性腹泻组(110只).对照组按每只大鼠1ml/d生理盐水灌胃;其余大鼠给予40g/L的甲状腺片混悬液,每只大鼠1 ml/d.根据大鼠血清FT3、FT4水平、体质量和湿粪的数量筛选出40只甲亢性腹泻大鼠.将甲亢性腹泻大鼠按体质量随机分为腹泻对照组、黄连素组,阿是止泻胶囊低剂量组、中剂量组、高剂量组,每组8只.腹泻对照组:按1 ml/d生理盐水给药;黄连素组:按1.94 g·kg-1·d-1给黄连素片;低、中、高剂量组:分别按0.63、1.26、2.52 g·kg-1·d-1给予阿是止泻胶囊.各组连续灌胃7 d,次日脱颈处死,取十二指肠、空肠、回肠、结肠组织,HE染色,光镜观察组织形态及杯状细胞的计数.细胞计数单位为细胞数/高倍镜下每视野(cells/hpf).结果 在空肠中,除低剂量组[(23.98±2.28)cells/hpf]外,黄连素组、中剂量组、高剂量组杯状细胞数量[(15.32±2.53)、(20.24±1.24)、(14.98±1.10)cells/hpf]均低于腹泻对照组[(25.73±4.55)cells/hpf,P均<0.05];结肠中,黄连素组、低剂量组、中剂量组、高剂量组结肠杯状细胞数量[(18.29 ±1.33)、(20.61±2.12)、(19.38±2.01)、(16.34±1.55)cells/hpf]均低于腹泻对照组[(23.36±3.10)cells/hpf,P均<0.05].空肠和结肠腹泻对照组和高剂量组均低于低剂量组(P均<0.05).十二指肠和回肠中杯状细胞数量组间比较差异无统计学意义(F=2.81、2.67,P均>0.05).形态学观察显示,空场和结肠组织中,腹泻对照组杯状细胞数量明显增多,而随着治疗剂量的增加细胞数量逐渐减少.结论 甲亢性腹泻大鼠空肠和结肠组织中杯状细胞数量明显增多,阿是止泻胶囊能显著降低空肠和结肠组织中杯状细胞数量,减少黏液分泌.
目的 探討阿是止瀉膠囊對甲狀腺功能亢進性腹瀉(簡稱甲亢性腹瀉)大鼠胃腸道杯狀細胞的影響.方法 8週齡的雄性SD大鼠120隻,按體質量隨機分為對照組(10隻)和甲亢性腹瀉組(110隻).對照組按每隻大鼠1ml/d生理鹽水灌胃;其餘大鼠給予40g/L的甲狀腺片混懸液,每隻大鼠1 ml/d.根據大鼠血清FT3、FT4水平、體質量和濕糞的數量篩選齣40隻甲亢性腹瀉大鼠.將甲亢性腹瀉大鼠按體質量隨機分為腹瀉對照組、黃連素組,阿是止瀉膠囊低劑量組、中劑量組、高劑量組,每組8隻.腹瀉對照組:按1 ml/d生理鹽水給藥;黃連素組:按1.94 g·kg-1·d-1給黃連素片;低、中、高劑量組:分彆按0.63、1.26、2.52 g·kg-1·d-1給予阿是止瀉膠囊.各組連續灌胃7 d,次日脫頸處死,取十二指腸、空腸、迴腸、結腸組織,HE染色,光鏡觀察組織形態及杯狀細胞的計數.細胞計數單位為細胞數/高倍鏡下每視野(cells/hpf).結果 在空腸中,除低劑量組[(23.98±2.28)cells/hpf]外,黃連素組、中劑量組、高劑量組杯狀細胞數量[(15.32±2.53)、(20.24±1.24)、(14.98±1.10)cells/hpf]均低于腹瀉對照組[(25.73±4.55)cells/hpf,P均<0.05];結腸中,黃連素組、低劑量組、中劑量組、高劑量組結腸杯狀細胞數量[(18.29 ±1.33)、(20.61±2.12)、(19.38±2.01)、(16.34±1.55)cells/hpf]均低于腹瀉對照組[(23.36±3.10)cells/hpf,P均<0.05].空腸和結腸腹瀉對照組和高劑量組均低于低劑量組(P均<0.05).十二指腸和迴腸中杯狀細胞數量組間比較差異無統計學意義(F=2.81、2.67,P均>0.05).形態學觀察顯示,空場和結腸組織中,腹瀉對照組杯狀細胞數量明顯增多,而隨著治療劑量的增加細胞數量逐漸減少.結論 甲亢性腹瀉大鼠空腸和結腸組織中杯狀細胞數量明顯增多,阿是止瀉膠囊能顯著降低空腸和結腸組織中杯狀細胞數量,減少黏液分泌.
목적 탐토아시지사효낭대갑상선공능항진성복사(간칭갑항성복사)대서위장도배상세포적영향.방법 8주령적웅성SD대서120지,안체질량수궤분위대조조(10지)화갑항성복사조(110지).대조조안매지대서1ml/d생리염수관위;기여대서급여40g/L적갑상선편혼현액,매지대서1 ml/d.근거대서혈청FT3、FT4수평、체질량화습분적수량사선출40지갑항성복사대서.장갑항성복사대서안체질량수궤분위복사대조조、황련소조,아시지사효낭저제량조、중제량조、고제량조,매조8지.복사대조조:안1 ml/d생리염수급약;황련소조:안1.94 g·kg-1·d-1급황련소편;저、중、고제량조:분별안0.63、1.26、2.52 g·kg-1·d-1급여아시지사효낭.각조련속관위7 d,차일탈경처사,취십이지장、공장、회장、결장조직,HE염색,광경관찰조직형태급배상세포적계수.세포계수단위위세포수/고배경하매시야(cells/hpf).결과 재공장중,제저제량조[(23.98±2.28)cells/hpf]외,황련소조、중제량조、고제량조배상세포수량[(15.32±2.53)、(20.24±1.24)、(14.98±1.10)cells/hpf]균저우복사대조조[(25.73±4.55)cells/hpf,P균<0.05];결장중,황련소조、저제량조、중제량조、고제량조결장배상세포수량[(18.29 ±1.33)、(20.61±2.12)、(19.38±2.01)、(16.34±1.55)cells/hpf]균저우복사대조조[(23.36±3.10)cells/hpf,P균<0.05].공장화결장복사대조조화고제량조균저우저제량조(P균<0.05).십이지장화회장중배상세포수량조간비교차이무통계학의의(F=2.81、2.67,P균>0.05).형태학관찰현시,공장화결장조직중,복사대조조배상세포수량명현증다,이수착치료제량적증가세포수량축점감소.결론 갑항성복사대서공장화결장조직중배상세포수량명현증다,아시지사효낭능현저강저공장화결장조직중배상세포수량,감소점액분비.
Objective To study the effect of Asi-antidiarrheal capsule on gastrointestinal goblet cell of thyroid hormone-induced diarrhea.Methods Total of 120 SD male rats aged about 8 weeks were randomly divided into 2 groups:control group(10 rats)and thyroid hormone-induced diarrheic group(110 rats).Rats in control group were lavaged with normal saline 1 ml/d.Thyroid tablets were partly desolved into normal saline forming a 40 mg/ml suspension.Rats in thyroid hormone-induced diarrheic group were given the thyroid suspension 1 ml/d to make thyroid hormone-induced diarrheic model.Serum FT3 and FT4 were tested.Fourty thyroid hormone-induced diarrheic rats were screened out according to serum FT3 and FT4 levels,body weight and wet stool.The fourty rats were randomly divided into 5 groups,8 rats in each group:positive control group,berberine group,low-dose,mediandose and high-dose groups.Normal saline of 1 ml/d was admnistered to diarrhea control group,1.94 g·kg-1·d-1 Berberine capsule was given to positive control group,and 0.63,1.26,2.52 g·kg-1·d-1 Asi-antidiarrheal capsule to low-dose,mediandose and high-dose groups,respectively.After sever days treatment,rats are executed.Duodenum,jejunum,ileum and colon were dissected,respectively.Histology observation and cell counting were carried out under light micmscopo on HE coloration.Cell counting unit was defined as:cell/high power field of vision (cells/hpf).Results In jejunum,the number of goblet cells in berberine group,mediandose group and high-dose group[(15.32±2.53),(20.24±1.24),(14.98±1.10)cells/hpf,respectively],were all lower than that of the diarrhea control group[(25.73±4.55)cells/hpf,all P<0.05]with an exception of low-dose group[(23.98±2.28)cells/hpf].The numbers of goblet cells in berberine control group,low-dose group,mediandose group and highdose group[(18.29±1.33),(20.61±2.12),(19.38±2.01),(16.34±1.55)cells/hpf,respectively]were all less than that of the control group[(23.36±3.10)cells/hpf,all P<0.05].The numbers of goblet cells of diarrhea control group and high-dose group were obviously lower than that of the low-dose group(all P<0.05)in jejunum and colon.The numbers of goblet cells of Duodenum and ileum were not significantly different between groups(F=2.81,2.67,all P>0.05).The numbers of goblet cells in the diarrhea control group increased markedly observed under microscope,but decreased following therapeutic treatment.Conclusions The numbers of goblet cells from jejunum and colon in thyroid hormone-induced diarrheic rats are increased significantly.Asi-antidiarrheal capsule can remarkably decrease the number of goblet cells in jejunum and colon,and reduce mucus secretion.
