CAJ | 학술논문

目的探讨强脉冲光照射对昆明小鼠皮肤胶原纤维、弹性纤维、透明质酸含量的意义.方法用强脉冲光照射小鼠右侧背部皮肤,左侧为对照,取各时间点照射组和对照组皮肤进行组织学观察,包括HE染色、Ⅰ型和Ⅲ型胶原纤维饱和苦味酸-天狼猩红染色、弹性纤维Gomori醛品红法染色,同时提取皮肤组织,碱水解-分光光度法测定羟脯氨酸含量,放射免疫法测定透明质酸含量.结果照射后2周,真皮厚度较对照组增加(t=4.623,P＜0.05),4周时达最大值,较对照组增加18.71%,8周时仍大于对照组,差异有统计学意义(t=3.904,P＜0.05).照射后1周,Ⅲ型胶原纤维较对照组增加40.54%(t=5.129,P＜0.05),照射后2周和4周,Ⅰ型和Ⅲ型胶原纤维均较对照组增多明显,至8周时,均大于对照组(P＜0.05).照射后2～8周,弹性纤维均较对照组增多(P＜0.05);照射后1～8周,羟脯氨酸含量均较对照组增多(P＜0.05).照射后1 d和3 d,透明质酸含量显著增加,1～8周,渐进性降低,但均高于对照组(P＜0.05).结论强脉冲光照射小鼠皮肤可刺激真皮胶原纤维、弹性纤维、透明质酸含量增加.
목적 탐토강맥충광조사대곤명소서피부효원섬유、탄성섬유、투명질산함량적의의.방법 용강맥충광조사소서우측배부피부,좌측위대조,취각시간점조사조화대조조피부진행조직학관찰,포괄HE염색、Ⅰ형화Ⅲ형효원섬유포화고미산-천랑성홍염색、탄성섬유Gomori철품홍법염색,동시제취피부조직,감수해-분광광도법측정간포안산함량,방사면역법측정투명질산함량.결과 조사후2주,진피후도교대조조증가(t=4.623,P＜0.05),4주시체최대치,교대조조증가18.71%,8주시잉대우대조조,차이유통계학의의(t=3.904,P＜0.05).조사후1주,Ⅲ형효원섬유교대조조증가40.54%(t=5.129,P＜0.05),조사후2주화4주,Ⅰ형화Ⅲ형효원섬유균교대조조증다명현,지8주시,균대우대조조(P＜0.05).조사후2～8주,탄성섬유균교대조조증다(P＜0.05);조사후1～8주,간포안산함량균교대조조증다(P＜0.05).조사후1 d화3 d,투명질산함량현저증가,1～8주,점진성강저,단균고우대조조(P＜0.05).결론 강맥충광조사소서피부가자격진피효원섬유、탄성섬유、투명질산함량증가.
Objective To investigate the effects of intense pulsed light (IPL) irradiation on the content of collagen fibers, elastic fibers and hyaluronic acid in Kunming mouse skin. Methods The dorsal skin of mice was divided into two areas: the right area was irradiated with IPL, and the left remaining unirradiated served as the control. Skin specimens were taken from the back of mice on day 1, 3, week 1, 2, 4 and 8 after the irradiation and subjected to staining with HE, sirius red and Gomori aldehyde-fuchsin for examinations of histological changes, type Ⅰ and Ⅲ collagen fibers and elastic fibers. The hydroxyproline and hyaluronic acid content in skin tissues of mice was determined with ultraviolet spectrophotometry and radioimmunoassay respectively. Results After irradiation, a significant increase was observed in dermal thickness on week 2 (t =4.623, P＜ 0.05), 4, and 8 (t = 3.904, P＜ 0.05), in type Ⅲ collagen fiber (t = 5.129, P＜ 0.05) on week 1,in type Ⅰ and Ⅲ collagen fibers on week 2, 4 and 8 (both P＜ 0.05), in elastic fibers from week 2 to 8 (P ＜0.05), and in hydroxyproline content from week 1 to 8 (all P ＜ 0.05) in the skin of mice compared with unirradiated mice. In detail, dermal thickness increased by 18.71% on week 4, and type Ⅲ collagen fiber by 40.54% in irradiated mice compared with unirradiated mice. Further more, the hyaluronic acid content was elevated from day 1 to 3, but gradually declined from week 1 to 8, and remained statistically higher from day 1 to week 8 (P ＜ 0.05) in irradiated mice compared to unirradiated mice. Conclusion IPL irradiation could induce an increase in the content of collagen fiber, elastic fiber and hyaluronic acid in the dorsal skin of mice.